PCNA1_ARATH
ID PCNA1_ARATH Reviewed; 263 AA.
AC Q9M7Q7; A7UIK5; Q9LNV6;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2001, sequence version 2.
DT 25-MAY-2022, entry version 139.
DE RecName: Full=Proliferating cellular nuclear antigen 1;
DE Short=PCNA 1;
GN Name=PCNA; Synonyms=PCNA1; OrderedLocusNames=At1g07370; ORFNames=F22G5.29;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Park S.C., Park E.H., Cho J.W.;
RT "Cloning and characterization of Arabidopsis proliferating cellular nuclear
RT antigen (PCNA).";
RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Di Rubbo A., Vonarx E.J., Kunz B.A.;
RL Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INTERACTION WITH ATXR5 AND ATXR6.
RX PubMed=16771839; DOI=10.1111/j.1365-313x.2006.02799.x;
RA Raynaud C., Sozzani R., Glab N., Domenichini S., Perennes C., Cella R.,
RA Kondorosi E., Bergounioux C.;
RT "Two cell-cycle regulated SET-domain proteins interact with proliferating
RT cell nuclear antigen (PCNA) in Arabidopsis.";
RL Plant J. 47:395-407(2006).
RN [8]
RP INTERACTION WITH POLH.
RX PubMed=18494853; DOI=10.1111/j.1365-313x.2008.03562.x;
RA Anderson H.J., Vonarx E.J., Pastushok L., Nakagawa M., Katafuchi A.,
RA Gruz P., Di Rubbo A., Grice D.M., Osmond M.J., Sakamoto A.N., Nohmi T.,
RA Xiao W., Kunz B.A.;
RT "Arabidopsis thaliana Y-family DNA polymerase eta catalyses translesion
RT synthesis and interacts functionally with PCNA2.";
RL Plant J. 55:895-908(2008).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1-256 IN COMPLEX WITH HUMAN P21
RP PEPTIDE, AND SUBUNIT.
RX PubMed=19388052; DOI=10.1002/pro.117;
RA Strzalka W., Oyama T., Tori K., Morikawa K.;
RT "Crystal structures of the Arabidopsis thaliana proliferating cell nuclear
RT antigen 1 and 2 proteins complexed with the human p21 C-terminal segment.";
RL Protein Sci. 18:1072-1080(2009).
CC -!- FUNCTION: This protein is an auxiliary protein of DNA polymerase delta
CC and is involved in the control of eukaryotic DNA replication by
CC increasing the polymerase's processibility during elongation of the
CC leading strand. {ECO:0000250}.
CC -!- SUBUNIT: Homo- and heterotrimer. Interacts with POLH, ATXR5 and ATXR6.
CC {ECO:0000269|PubMed:16771839, ECO:0000269|PubMed:18494853,
CC ECO:0000269|PubMed:19388052}.
CC -!- INTERACTION:
CC Q9M7Q7; Q8H2D5: POLH; NbExp=2; IntAct=EBI-1810458, EBI-1810451;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the PCNA family. {ECO:0000305}.
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DR EMBL; AF083220; AAF40018.1; -; mRNA.
DR EMBL; EU072917; ABU25233.1; -; mRNA.
DR EMBL; AC022464; AAF79566.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28115.1; -; Genomic_DNA.
DR EMBL; AF462821; AAL58911.1; -; mRNA.
DR EMBL; AY098969; AAM19979.1; -; mRNA.
DR EMBL; AY086852; AAM63900.1; -; mRNA.
DR RefSeq; NP_172217.1; NM_100611.4.
DR PDB; 2ZVV; X-ray; 2.00 A; A/B=1-256.
DR PDB; 6O09; X-ray; 2.06 A; A/C/D/F/H/K=1-263.
DR PDBsum; 2ZVV; -.
DR PDBsum; 6O09; -.
DR AlphaFoldDB; Q9M7Q7; -.
DR SMR; Q9M7Q7; -.
DR BioGRID; 22490; 46.
DR IntAct; Q9M7Q7; 7.
DR STRING; 3702.AT1G07370.1; -.
DR iPTMnet; Q9M7Q7; -.
DR PaxDb; Q9M7Q7; -.
DR PRIDE; Q9M7Q7; -.
DR ProteomicsDB; 236846; -.
DR EnsemblPlants; AT1G07370.1; AT1G07370.1; AT1G07370.
DR GeneID; 837249; -.
DR Gramene; AT1G07370.1; AT1G07370.1; AT1G07370.
DR KEGG; ath:AT1G07370; -.
DR Araport; AT1G07370; -.
DR TAIR; locus:2025062; AT1G07370.
DR eggNOG; KOG1636; Eukaryota.
DR HOGENOM; CLU_043978_3_0_1; -.
DR InParanoid; Q9M7Q7; -.
DR OMA; EMKLINM; -.
DR OrthoDB; 1012066at2759; -.
DR PhylomeDB; Q9M7Q7; -.
DR EvolutionaryTrace; Q9M7Q7; -.
DR PRO; PR:Q9M7Q7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9M7Q7; baseline and differential.
DR Genevisible; Q9M7Q7; AT.
DR GO; GO:0005737; C:cytoplasm; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0043626; C:PCNA complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030337; F:DNA polymerase processivity factor activity; IBA:GO_Central.
DR GO; GO:0006272; P:leading strand elongation; IBA:GO_Central.
DR GO; GO:0006298; P:mismatch repair; IBA:GO_Central.
DR GO; GO:0051726; P:regulation of cell cycle; ISS:TAIR.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:InterPro.
DR GO; GO:0019985; P:translesion synthesis; IBA:GO_Central.
DR HAMAP; MF_00317; DNApol_clamp_arch; 1.
DR IDEAL; IID50102; -.
DR InterPro; IPR000730; Pr_cel_nuc_antig.
DR InterPro; IPR022649; Pr_cel_nuc_antig_C.
DR InterPro; IPR022659; Pr_cel_nuc_antig_CS.
DR InterPro; IPR022648; Pr_cel_nuc_antig_N.
DR Pfam; PF02747; PCNA_C; 1.
DR Pfam; PF00705; PCNA_N; 1.
DR PRINTS; PR00339; PCNACYCLIN.
DR TIGRFAMs; TIGR00590; pcna; 1.
DR PROSITE; PS01251; PCNA_1; 1.
DR PROSITE; PS00293; PCNA_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA replication; DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..263
FT /note="Proliferating cellular nuclear antigen 1"
FT /id="PRO_0000149177"
FT DNA_BIND 61..80
FT /evidence="ECO:0000255"
FT CONFLICT 48
FT /note="V -> A (in Ref. 1; AAF40018 and 2; ABU25233)"
FT /evidence="ECO:0000305"
FT STRAND 2..7
FT /evidence="ECO:0007829|PDB:2ZVV"
FT HELIX 10..19
FT /evidence="ECO:0007829|PDB:2ZVV"
FT TURN 20..22
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 24..31
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 34..40
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 44..53
FT /evidence="ECO:0007829|PDB:2ZVV"
FT HELIX 54..56
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 57..64
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 66..71
FT /evidence="ECO:0007829|PDB:2ZVV"
FT HELIX 72..80
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 87..92
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 97..104
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 111..117
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 134..140
FT /evidence="ECO:0007829|PDB:2ZVV"
FT HELIX 141..152
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 156..163
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 166..173
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 176..182
FT /evidence="ECO:0007829|PDB:2ZVV"
FT HELIX 191..193
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 196..201
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 203..208
FT /evidence="ECO:0007829|PDB:2ZVV"
FT HELIX 209..215
FT /evidence="ECO:0007829|PDB:2ZVV"
FT HELIX 216..221
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 223..229
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 235..241
FT /evidence="ECO:0007829|PDB:2ZVV"
FT TURN 242..244
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 245..251
FT /evidence="ECO:0007829|PDB:2ZVV"
FT STRAND 254..256
FT /evidence="ECO:0007829|PDB:6O09"
SQ SEQUENCE 263 AA; 29122 MW; DA63C0F637CDB5D7 CRC64;
MLELRLVQGS LLKKVLESIK DLVNDANFDC SSTGFSLQAM DSSHVALVSL LLRSEGFEHY
RCDRNLSMGM NLGNMSKMLK CAGNDDIITI KADDGGDTVT FMFESPTQDK IADFEMKLMD
IDSEHLGIPD AEYHSIVRMP SNEFSRICKD LSSIGDTVVI SVTKEGVKFS TAGDIGTANI
VLRQNTTVDK PEDAIVIEMK EPVSLSFALR YMNSFTKATP LSDTVTISLS SELPVVVEYK
VAEMGYIRYY LAPKIEEEED TNP
