PCNA1_PLAFK
ID PCNA1_PLAFK Reviewed; 274 AA.
AC P31008;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Proliferating cell nuclear antigen 1 {ECO:0000303|PubMed:26911594};
DE Short=PfPCNA1 {ECO:0000303|PubMed:26911594};
GN Name=PCNA1 {ECO:0000303|PubMed:26911594};
GN Synonyms=PCNA {ECO:0000303|PubMed:8095096};
OS Plasmodium falciparum (isolate K1 / Thailand).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=5839;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8095096; DOI=10.1093/nar/21.2.239;
RA Kilbey B.J., Fraser I., McAleese S., Goman M., Ridley R.G.;
RT "Molecular characterisation and stage-specific expression of proliferating
RT cell nuclear antigen (PCNA) from the malarial parasite, Plasmodium
RT falciparum.";
RL Nucleic Acids Res. 21:239-243(1993).
RN [2]
RP DEVELOPMENTAL STAGE.
RX PubMed=8855554; DOI=10.1016/0166-6851(96)02657-6;
RA Horrocks P., Jackson M., Cheesman S., White J.H., Kilbey B.J.;
RT "Stage specific expression of proliferating cell nuclear antigen and DNA
RT polymerase delta from Plasmodium falciparum.";
RL Mol. Biochem. Parasitol. 79:177-182(1996).
RN [3]
RP FUNCTION.
RX PubMed=26911594; DOI=10.1186/s12936-016-1166-0;
RA Vasuvat J., Montree A., Moonsom S., Leartsakulpanich U., Petmitr S.,
RA Focher F., Wright G.E., Chavalitshewinkoon-Petmitr P.;
RT "Biochemical and functional characterization of Plasmodium falciparum DNA
RT polymerase delta.";
RL Malar. J. 15:116-116(2016).
CC -!- FUNCTION: Auxiliary protein of DNA polymerase delta and is involved in
CC the control of DNA replication by increasing the polymerase
CC processibility during elongation of the leading strand
CC (PubMed:26911594). Involved in DNA damage response (By similarity).
CC {ECO:0000250|UniProtKB:P61074, ECO:0000269|PubMed:26911594}.
CC -!- SUBUNIT: Homotrimer. Interacts with ORC1 (via PIP-box motif); the
CC interaction occurs during DNA replication in trophozoites. Interacts
CC with ORC5; the interaction occurs during the trophozoite stage but not
CC at the late schizont stage. Interacts with FEN1.
CC {ECO:0000250|UniProtKB:P61074}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P61074}.
CC Chromosome {ECO:0000250|UniProtKB:P61074}. Cytoplasm
CC {ECO:0000250|UniProtKB:P61074}. Note=During early-to mid replicating
CC trophozoite stages, colocalizes with ORC1 and ORC5 to distinct nuclear
CC foci which probably are DNA replication origin sites. Dissociates from
CC ORC1 and ORC5 during the late schizont stage.
CC {ECO:0000250|UniProtKB:P61074}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the asexual blood stage
CC specifically in trophozoites and schizonts (at protein level).
CC {ECO:0000269|PubMed:8855554}.
CC -!- SIMILARITY: Belongs to the PCNA family. {ECO:0000305}.
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DR EMBL; X68739; CAA48673.1; -; Genomic_DNA.
DR PIR; S30224; S30224.
DR AlphaFoldDB; P31008; -.
DR SMR; P31008; -.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030337; F:DNA polymerase processivity factor activity; IDA:UniProtKB.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:1900264; P:positive regulation of DNA-directed DNA polymerase activity; IDA:UniProtKB.
DR HAMAP; MF_00317; DNApol_clamp_arch; 1.
DR InterPro; IPR000730; Pr_cel_nuc_antig.
DR InterPro; IPR022649; Pr_cel_nuc_antig_C.
DR InterPro; IPR022659; Pr_cel_nuc_antig_CS.
DR InterPro; IPR022648; Pr_cel_nuc_antig_N.
DR PANTHER; PTHR11352:SF0; PTHR11352:SF0; 1.
DR Pfam; PF02747; PCNA_C; 1.
DR Pfam; PF00705; PCNA_N; 1.
DR PRINTS; PR00339; PCNACYCLIN.
DR TIGRFAMs; TIGR00590; pcna; 1.
DR PROSITE; PS01251; PCNA_1; 1.
DR PROSITE; PS00293; PCNA_2; 1.
PE 1: Evidence at protein level;
KW Chromosome; Cytoplasm; DNA replication; DNA-binding; Nucleus.
FT CHAIN 1..274
FT /note="Proliferating cell nuclear antigen 1"
FT /id="PRO_0000149174"
FT DNA_BIND 61..80
FT /evidence="ECO:0000255"
FT SITE 213
FT /note="May be important for nuclear localization"
FT /evidence="ECO:0000250|UniProtKB:P61074"
SQ SEQUENCE 274 AA; 30587 MW; E3128F7397DD1687 CRC64;
MLEAKLNNAS ILKKLFECIK DLVNDANVDA DESGLKLQAL DGNHVSLVSL HLLDSGFSHY
RCDRERVLGV NIASLNKVFK LCGANESVVI SSKDDEDNLN FVFENNKEDK VTNFSLKLMS
IELDSLNIPD CEEGFDAEVE LSSKELTNIF RNLSEFSDTV FIEIDSNCIK FTTKGIVGDA
EVALKPRDST SEDDIGVTIK SKKKIKQSFA IKYLNLFSKS NILADVVVLG LSDSRPIEFK
YEIKDTSPDS DTLKIGFVKF FLAPKMDDDM DNKD
