PCNA2_DROME
ID PCNA2_DROME Reviewed; 255 AA.
AC Q9VIT0;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Proliferating cell nuclear antigen 2 {ECO:0000303|PubMed:17087725};
GN Name=PCNA2 {ECO:0000303|PubMed:17087725, ECO:0000312|FlyBase:FBgn0032813};
GN ORFNames=CG10262 {ECO:0000312|FlyBase:FBgn0032813};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:BAE94851.1}
RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, INTERACTION WITH POLD1 AND POLE1,
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=17087725; DOI=10.1111/j.1742-4658.2006.05504.x;
RA Ruike T., Takeuchi R., Takata K., Oshige M., Kasai N., Shimanouchi K.,
RA Kanai Y., Nakamura R., Sugawara F., Sakaguchi K.;
RT "Characterization of a second proliferating cell nuclear antigen (PCNA2)
RT from Drosophila melanogaster.";
RL FEBS J. 273:5062-5073(2006).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Likely to be an auxiliary protein of DNA polymerase delta
CC complex and is probably involved in the control of DNA replication and
CC repair by increasing the polymerase's processibility (PubMed:17087725).
CC May function independently of PCNA during DNA repair (PubMed:17087725).
CC {ECO:0000269|PubMed:17087725}.
CC -!- SUBUNIT: Homotrimer (PubMed:17087725). Interacts with the catalytic
CC subunits of two DNA polymerase complexes: PolD1 in the delta complex
CC and PolE1/DNApol-epsilon255 in the epsilon complex (PubMed:17087725).
CC {ECO:0000269|PubMed:17087725}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17087725}. Chromosome
CC {ECO:0000269|PubMed:17087725}. Cytoplasm {ECO:0000269|PubMed:17087725}.
CC Note=Increased association with chromatin in response to DNA damage
CC caused by methyl methanesulfonate (MMS) and hydrogen peroxide (H2O2),
CC but not in response to DNA damage by UV. {ECO:0000269|PubMed:17087725}.
CC -!- DEVELOPMENTAL STAGE: Ubiquitously expressed throughout development and
CC in adults. {ECO:0000269|PubMed:17087725}.
CC -!- SIMILARITY: Belongs to the PCNA family. {ECO:0000305}.
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DR EMBL; AB195794; BAE94851.1; -; mRNA.
DR EMBL; AE014134; AAF53835.1; -; Genomic_DNA.
DR RefSeq; NP_609994.1; NM_136150.3.
DR AlphaFoldDB; Q9VIT0; -.
DR SMR; Q9VIT0; -.
DR DIP; DIP-17763N; -.
DR IntAct; Q9VIT0; 12.
DR STRING; 7227.FBpp0080803; -.
DR PaxDb; Q9VIT0; -.
DR EnsemblMetazoa; FBtr0081262; FBpp0080803; FBgn0032813.
DR GeneID; 35257; -.
DR KEGG; dme:Dmel_CG10262; -.
DR UCSC; CG10262-RA; d. melanogaster.
DR CTD; 35257; -.
DR FlyBase; FBgn0032813; PCNA2.
DR VEuPathDB; VectorBase:FBgn0032813; -.
DR eggNOG; KOG1636; Eukaryota.
DR GeneTree; ENSGT00390000004965; -.
DR HOGENOM; CLU_043978_3_0_1; -.
DR InParanoid; Q9VIT0; -.
DR OMA; KERTADY; -.
DR OrthoDB; 1012066at2759; -.
DR PhylomeDB; Q9VIT0; -.
DR SignaLink; Q9VIT0; -.
DR BioGRID-ORCS; 35257; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35257; -.
DR PRO; PR:Q9VIT0; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032813; Expressed in saliva-secreting gland and 13 other tissues.
DR ExpressionAtlas; Q9VIT0; baseline and differential.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0043626; C:PCNA complex; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0070182; F:DNA polymerase binding; IDA:FlyBase.
DR GO; GO:0030337; F:DNA polymerase processivity factor activity; IBA:GO_Central.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IDA:UniProtKB.
DR GO; GO:0006272; P:leading strand elongation; IBA:GO_Central.
DR GO; GO:0006298; P:mismatch repair; IBA:GO_Central.
DR GO; GO:0006275; P:regulation of DNA replication; IEA:InterPro.
DR GO; GO:0042542; P:response to hydrogen peroxide; IDA:UniProtKB.
DR GO; GO:0072702; P:response to methyl methanesulfonate; IDA:UniProtKB.
DR GO; GO:0019985; P:translesion synthesis; IBA:GO_Central.
DR HAMAP; MF_00317; DNApol_clamp_arch; 1.
DR InterPro; IPR000730; Pr_cel_nuc_antig.
DR InterPro; IPR022649; Pr_cel_nuc_antig_C.
DR InterPro; IPR022659; Pr_cel_nuc_antig_CS.
DR InterPro; IPR022648; Pr_cel_nuc_antig_N.
DR PANTHER; PTHR11352:SF0; PTHR11352:SF0; 1.
DR Pfam; PF02747; PCNA_C; 1.
DR Pfam; PF00705; PCNA_N; 1.
DR PRINTS; PR00339; PCNACYCLIN.
DR TIGRFAMs; TIGR00590; pcna; 1.
DR PROSITE; PS01251; PCNA_1; 1.
PE 1: Evidence at protein level;
KW Chromosome; Cytoplasm; DNA replication; DNA-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..255
FT /note="Proliferating cell nuclear antigen 2"
FT /id="PRO_0000448932"
FT DNA_BIND 61..80
FT /evidence="ECO:0000255"
SQ SEQUENCE 255 AA; 28461 MW; BE7DCA785E28C882 CRC64;
MLEARLSQTL LLKKIVDALK EIIAQGTLDC SENGLELQSM DNSHVSLVAL SLASDCFEKF
HCDRNVSLGL DLKSLGKVLK CANSDDAVTI KAVDRPEKIT LSFESDGKER TADYELKLLN
LDQDHMEIPK KDYTCFIQLP SSEFARICRD MSMFDESLTI ACSSKGIRFL AKGDLGTANI
QLSAGTAMDV SIEVQEPVTQ SFAGRYLNTF TKATPLADRV KLYLSDERPL LVEYPIEDYG
HIRYYLAPKV NDPDF
