PCNA_ORYSJ
ID PCNA_ORYSJ Reviewed; 263 AA.
AC P17070; Q0DWN6; Q6K845; Q7G870;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Proliferating cell nuclear antigen;
DE Short=OsPCNA {ECO:0000303|PubMed:11851910};
DE Short=PCNA;
DE AltName: Full=Cyclin;
GN OrderedLocusNames=Os02g0805200, LOC_Os02g56130;
GN ORFNames=OJ1548_F12.18, OsJ_08777 {ECO:0000312|EMBL:EAZ24997.1},
GN OSJNBa0049O12.19;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1671766; DOI=10.1111/j.1432-1033.1991.tb15739.x;
RA Suzuka I., Hata S., Matsuoka M., Kosugi S., Hashimoto J.;
RT "Highly conserved structure of proliferating cell nuclear antigen (DNA
RT polymerase delta auxiliary protein) gene in plants.";
RL Eur. J. Biochem. 195:571-575(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RG The rice full-length cDNA consortium;
RT "Oryza sativa full length cDNA.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 108-157.
RX PubMed=2566167; DOI=10.1073/pnas.86.9.3189;
RA Suzuka I., Daidoji H., Matsuoka M., Kadowaki K., Takasaki Y., Nakane P.K.,
RA Moriuchi T.;
RT "Gene for proliferating-cell nuclear antigen (DNA polymerase delta
RT auxiliary protein) is present in both mammalian and higher plant genomes.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:3189-3193(1989).
RN [9]
RP SUBUNIT, INTERACTION WITH FEN1A, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND INDUCTION.
RX PubMed=11851910; DOI=10.1046/j.1365-313x.2001.01184.x;
RA Kimura S., Suzuki T., Yanagawa Y., Yamamoto T., Nakagawa H., Tanaka I.,
RA Hashimoto J., Sakaguchi K.;
RT "Characterization of plant proliferating cell nuclear antigen (PCNA) and
RT flap endonuclease-1 (FEN-1), and their distribution in mitotic and meiotic
RT cell cycles.";
RL Plant J. 28:643-653(2001).
RN [10]
RP INTERACTION WITH POLL.
RC STRAIN=cv. Nipponbare;
RX PubMed=15206945; DOI=10.1111/j.1432-1033.2004.04214.x;
RA Uchiyama Y., Kimura S., Yamamoto T., Ishibashi T., Sakaguchi K.;
RT "Plant DNA polymerase lambda, a DNA repair enzyme that functions in plant
RT meristematic and meiotic tissues.";
RL Eur. J. Biochem. 271:2799-2807(2004).
RN [11]
RP TISSUE SPECIFICITY.
RX PubMed=15150342; DOI=10.1093/nar/gkh591;
RA Kimura S., Tahira Y., Ishibashi T., Mori Y., Mori T., Hashimoto J.,
RA Sakaguchi K.;
RT "DNA repair in higher plants; photoreactivation is the major DNA repair
RT pathway in non-proliferating cells while excision repair (nucleotide
RT excision repair and base excision repair) is active in proliferating
RT cells.";
RL Nucleic Acids Res. 32:2760-2767(2004).
RN [12]
RP INTERACTION WITH DJA7 AND DJA8, AND INDUCTION.
RX PubMed=15806324; DOI=10.1007/s10265-005-0197-3;
RA Yamamoto T., Mori Y., Ishibashi T., Uchiyama Y., Ueda T., Ando T.,
RA Hashimoto J., Kimura S., Sakaguchi K.;
RT "Interaction between proliferating cell nuclear antigen (PCNA) and a DnaJ
RT induced by DNA damage.";
RL J. Plant Res. 118:91-97(2005).
RN [13]
RP INTERACTION WITH RAD/GEN1.
RX PubMed=22247560; DOI=10.1093/jb/mvr145;
RA Yang Y., Ishino S., Yamagami T., Kumamaru T., Satoh H., Ishino Y.;
RT "The OsGEN-L protein from Oryza sativa possesses Holliday junction
RT resolvase activity as well as 5'-flap endonuclease activity.";
RL J. Biochem. 151:317-327(2012).
RN [14]
RP INDUCTION BY GAMMA IRRADIATION.
RX PubMed=25124817; DOI=10.1093/jhered/esu025;
RA Hayashi G., Shibato J., Imanaka T., Cho K., Kubo A., Kikuchi S., Satoh K.,
RA Kimura S., Ozawa S., Fukutani S., Endo S., Ichikawa K., Agrawal G.K.,
RA Shioda S., Fukumoto M., Rakwal R.;
RT "Unraveling low-level gamma radiation--responsive changes in expression of
RT early and late genes in leaves of rice seedlings at Iitate Village,
RT Fukushima.";
RL J. Hered. 105:723-738(2014).
RN [15]
RP INDUCTION BY HEAVY ION IRRADIATION.
RX PubMed=27462908; DOI=10.1371/journal.pone.0160061;
RA Ishii K., Kazama Y., Morita R., Hirano T., Ikeda T., Usuda S., Hayashi Y.,
RA Ohbu S., Motoyama R., Nagamura Y., Abe T.;
RT "Linear energy transfer-dependent change in rice gene expression profile
RT after heavy-ion beam irradiation.";
RL PLoS ONE 11:E0160061-E0160061(2016).
CC -!- FUNCTION: This protein is an auxiliary protein of DNA polymerase delta
CC and is involved in the control of eukaryotic DNA replication by
CC increasing the polymerase's processibility during elongation of the
CC leading strand.
CC -!- SUBUNIT: Homotrimer. Interacts with FEN1A (PubMed:11851910). Interacts
CC with POLL (PubMed:15206945). Interacts with RAD/GEN1 (PubMed:22247560).
CC Interacts with DJA7 and DJA8 (PubMed:15806324).
CC {ECO:0000269|PubMed:11851910, ECO:0000269|PubMed:15206945,
CC ECO:0000269|PubMed:15806324, ECO:0000269|PubMed:22247560}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11851910}.
CC -!- TISSUE SPECIFICITY: Expressed in proliferating tissues. Expressed in
CC roots and root apex. Expressed at low levels in young leaves. Not
CC detected in mature leaves (PubMed:11851910, PubMed:15150342). Highly
CC expressed in shoot apical meristem (SAM). Expressed in flag leaves and
CC panicles (PubMed:15150342). {ECO:0000269|PubMed:11851910,
CC ECO:0000269|PubMed:15150342}.
CC -!- INDUCTION: Induced by the cell cycle inhibitors aphidicolin and
CC hydroxyurea (PubMed:11851910). Induced by gamma irradiation
CC (PubMed:25124817). Induced by heavy ion irradiation (PubMed:27462908).
CC Induced by UV and hydrogen peroxide. Down-regulated during sucrose
CC starvation (PubMed:15806324). {ECO:0000269|PubMed:11851910,
CC ECO:0000269|PubMed:15806324, ECO:0000269|PubMed:25124817,
CC ECO:0000269|PubMed:27462908}.
CC -!- SIMILARITY: Belongs to the PCNA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA33913.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X54046; CAA37979.1; -; Genomic_DNA.
DR EMBL; AC069158; AAK98707.1; -; Genomic_DNA.
DR EMBL; AP004240; BAD19422.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF10352.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS81457.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ24997.1; -; Genomic_DNA.
DR EMBL; AK071591; BAG92571.1; -; mRNA.
DR EMBL; AK243048; BAH01423.1; -; mRNA.
DR EMBL; J04538; AAA33913.1; ALT_SEQ; Genomic_DNA.
DR PIR; S14415; S14415.
DR RefSeq; XP_015627245.1; XM_015771759.1.
DR AlphaFoldDB; P17070; -.
DR SMR; P17070; -.
DR IntAct; P17070; 2.
DR STRING; 4530.OS02T0805200-01; -.
DR PaxDb; P17070; -.
DR PRIDE; P17070; -.
DR EnsemblPlants; Os02t0805200-01; Os02t0805200-01; Os02g0805200.
DR GeneID; 4331062; -.
DR Gramene; Os02t0805200-01; Os02t0805200-01; Os02g0805200.
DR KEGG; osa:4331062; -.
DR eggNOG; KOG1636; Eukaryota.
DR HOGENOM; CLU_043978_3_0_1; -.
DR InParanoid; P17070; -.
DR OMA; EMKLINM; -.
DR OrthoDB; 1012066at2759; -.
DR PlantReactome; R-OSA-9675782; Maturation.
DR PlantReactome; R-OSA-9675815; Leading strand synthesis.
DR PlantReactome; R-OSA-9675885; Lagging strand synthesis.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; P17070; OS.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0043626; C:PCNA complex; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0003684; F:damaged DNA binding; ISS:UniProtKB.
DR GO; GO:0030337; F:DNA polymerase processivity factor activity; IBA:GO_Central.
DR GO; GO:0034644; P:cellular response to UV; ISS:UniProtKB.
DR GO; GO:0006272; P:leading strand elongation; IBA:GO_Central.
DR GO; GO:0006298; P:mismatch repair; IBA:GO_Central.
DR GO; GO:0045739; P:positive regulation of DNA repair; ISS:UniProtKB.
DR GO; GO:0045740; P:positive regulation of DNA replication; ISS:UniProtKB.
DR GO; GO:0070207; P:protein homotrimerization; IPI:UniProtKB.
DR GO; GO:0019985; P:translesion synthesis; IBA:GO_Central.
DR HAMAP; MF_00317; DNApol_clamp_arch; 1.
DR InterPro; IPR000730; Pr_cel_nuc_antig.
DR InterPro; IPR022649; Pr_cel_nuc_antig_C.
DR InterPro; IPR022659; Pr_cel_nuc_antig_CS.
DR InterPro; IPR022648; Pr_cel_nuc_antig_N.
DR Pfam; PF02747; PCNA_C; 1.
DR Pfam; PF00705; PCNA_N; 1.
DR PRINTS; PR00339; PCNACYCLIN.
DR TIGRFAMs; TIGR00590; pcna; 1.
DR PROSITE; PS01251; PCNA_1; 1.
DR PROSITE; PS00293; PCNA_2; 1.
PE 1: Evidence at protein level;
KW DNA replication; DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..263
FT /note="Proliferating cell nuclear antigen"
FT /id="PRO_0000149184"
FT DNA_BIND 61..80
FT /evidence="ECO:0000255"
FT CONFLICT 144..145
FT /note="FS -> KT (in Ref. 8; AAA33913)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 263 AA; 29271 MW; F13B5DFA93079AAF CRC64;
MLELRLVQGS LLKKVLEAIR ELVTDANFDC SGTGFSLQAM DSSHVALVAL LLRSEGFEHY
RCDRNLSMGM NLNNMAKMLR CAGNDDIITI KADDGSDTVT FMFESPNQDK IADFEMKLMD
IDSEHLGIPD SEYQAIVRMP SSEFSRICKD LSSIGDTVII SVTKEGVKFS TAGDIGTANI
VCRQNKTVDK PEDATIIEMQ EPVSLTFALR YMNSFTKASP LSEQVTISLS SELPVVVEYK
IAEMGYIRFY LAPKIEEDEE MKS
