ID   PCNB_HAEIN              Reviewed;         452 AA.
AC   P44439;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Poly(A) polymerase I {ECO:0000255|HAMAP-Rule:MF_00957};
DE            Short=PAP I {ECO:0000255|HAMAP-Rule:MF_00957};
DE            EC=2.7.7.19 {ECO:0000255|HAMAP-Rule:MF_00957};
GN   Name=pcnB {ECO:0000255|HAMAP-Rule:MF_00957}; OrderedLocusNames=HI_0063;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- FUNCTION: Adds poly(A) tail to the 3' end of many RNAs, which usually
CC       targets these RNAs for decay. Plays a significant role in the global
CC       control of gene expression, through influencing the rate of transcript
CC       degradation, and in the general RNA quality control.
CC       {ECO:0000255|HAMAP-Rule:MF_00957}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide;
CC         Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17347,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:140395,
CC         ChEBI:CHEBI:173115; EC=2.7.7.19; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00957};
CC   -!- SIMILARITY: Belongs to the tRNA nucleotidyltransferase/poly(A)
CC       polymerase family. {ECO:0000255|HAMAP-Rule:MF_00957}.
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DR   EMBL; L42023; AAC21741.1; -; Genomic_DNA.
DR   PIR; B64046; B64046.
DR   RefSeq; NP_438236.1; NC_000907.1.
DR   RefSeq; WP_010868929.1; NC_000907.1.
DR   AlphaFoldDB; P44439; -.
DR   SMR; P44439; -.
DR   STRING; 71421.HI_0063; -.
DR   EnsemblBacteria; AAC21741; AAC21741; HI_0063.
DR   KEGG; hin:HI_0063; -.
DR   PATRIC; fig|71421.8.peg.64; -.
DR   eggNOG; COG0617; Bacteria.
DR   HOGENOM; CLU_015961_0_0_6; -.
DR   OMA; FMAKLDM; -.
DR   PhylomeDB; P44439; -.
DR   BioCyc; HINF71421:G1GJ1-64-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004652; F:polynucleotide adenylyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006378; P:mRNA polyadenylation; IEA:InterPro.
DR   CDD; cd05398; NT_ClassII-CCAase; 1.
DR   Gene3D; 3.30.460.10; -; 1.
DR   HAMAP; MF_00957; PolyA_pol; 1.
DR   InterPro; IPR043519; NT_sf.
DR   InterPro; IPR002646; PolA_pol_head_dom.
DR   InterPro; IPR010206; PolA_pol_I.
DR   InterPro; IPR025866; PolyA_pol_arg_C_dom.
DR   InterPro; IPR032828; PolyA_RNA-bd.
DR   Pfam; PF01743; PolyA_pol; 1.
DR   Pfam; PF12626; PolyA_pol_arg_C; 1.
DR   Pfam; PF12627; PolyA_pol_RNAbd; 1.
DR   SUPFAM; SSF81301; SSF81301; 1.
DR   TIGRFAMs; TIGR01942; pcnB; 1.
PE   3: Inferred from homology;
KW   ATP-binding; mRNA processing; Nucleotide-binding; Reference proteome;
KW   RNA-binding; Transcription; Transferase.
FT   CHAIN           1..452
FT                   /note="Poly(A) polymerase I"
FT                   /id="PRO_0000139093"
FT   REGION          427..452
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        68
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00957"
FT   ACT_SITE        70
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00957"
FT   ACT_SITE        150
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00957"
SQ   SEQUENCE   452 AA;  52698 MW;  1B3A9F9E2286F349 CRC64;
     MPKARAKKSE QTRRYDKNVI KAAQFDISPR DFSRNALNVV EKLQRQGFEA YIVGGCIRDL
     LLGKKPKDFD VATNARPEQI QNIFQRQCRL VGRRFRLAHI MFGRDIIEVA TFRANHSDAR
     NENQAKQSNE GMLLRDNVYG TIEQDAARRD FTVNALYYNP QDNTLRDYFE GIKDLKAGKL
     RLIGDPVTRY QEDPVRMLRS IRFMAKLDMF LEKPSEQPIR ELAPLLKNIP PARLFDESLK
     LLQAGQGVKT YRLLRQYGLF EQLFPALSAY FTEKEDSFAE RMIVTALTST DERVADKLRI
     NPAFLFAAFF WYPLREKVEI LKNEGGLNNH DAYALAGNEV LDLFCRALAA PRRHTAVIRD
     IWFLQLQLLK RTGSAPMRTM EHPKFRAGFD LLAMRAEIEG GETIELAKWW HEYQFSNGEQ
     REQLIQEQQR LHPKPKKKYY RPRRRKTTCS AE