ID   PCNB_SALTY              Reviewed;         465 AA.
AC   Q8ZRQ8;
DT   27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 2.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Poly(A) polymerase I {ECO:0000255|HAMAP-Rule:MF_00957};
DE            Short=PAP I {ECO:0000255|HAMAP-Rule:MF_00957};
DE            EC=2.7.7.19 {ECO:0000255|HAMAP-Rule:MF_00957};
GN   Name=pcnB {ECO:0000255|HAMAP-Rule:MF_00957}; OrderedLocusNames=STM0184;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Adds poly(A) tail to the 3' end of many RNAs, which usually
CC       targets these RNAs for decay. Plays a significant role in the global
CC       control of gene expression, through influencing the rate of transcript
CC       degradation, and in the general RNA quality control.
CC       {ECO:0000255|HAMAP-Rule:MF_00957}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide;
CC         Xref=Rhea:RHEA:11332, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17347,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:140395,
CC         ChEBI:CHEBI:173115; EC=2.7.7.19; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00957};
CC   -!- SIMILARITY: Belongs to the tRNA nucleotidyltransferase/poly(A)
CC       polymerase family. {ECO:0000255|HAMAP-Rule:MF_00957}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL19148.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE006468; AAL19148.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_459189.1; NC_003197.2.
DR   AlphaFoldDB; Q8ZRQ8; -.
DR   SMR; Q8ZRQ8; -.
DR   STRING; 99287.STM0184; -.
DR   PaxDb; Q8ZRQ8; -.
DR   EnsemblBacteria; AAL19148; AAL19148; STM0184.
DR   GeneID; 1251702; -.
DR   KEGG; stm:STM0184; -.
DR   PATRIC; fig|99287.12.peg.194; -.
DR   HOGENOM; CLU_015961_0_0_6; -.
DR   PhylomeDB; Q8ZRQ8; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004652; F:polynucleotide adenylyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006378; P:mRNA polyadenylation; IEA:InterPro.
DR   CDD; cd05398; NT_ClassII-CCAase; 1.
DR   Gene3D; 3.30.460.10; -; 1.
DR   HAMAP; MF_00957; PolyA_pol; 1.
DR   InterPro; IPR043519; NT_sf.
DR   InterPro; IPR002646; PolA_pol_head_dom.
DR   InterPro; IPR010206; PolA_pol_I.
DR   InterPro; IPR025866; PolyA_pol_arg_C_dom.
DR   InterPro; IPR032828; PolyA_RNA-bd.
DR   Pfam; PF01743; PolyA_pol; 1.
DR   Pfam; PF12626; PolyA_pol_arg_C; 1.
DR   Pfam; PF12627; PolyA_pol_RNAbd; 1.
DR   SUPFAM; SSF81301; SSF81301; 1.
DR   TIGRFAMs; TIGR01942; pcnB; 1.
PE   3: Inferred from homology;
KW   ATP-binding; mRNA processing; Nucleotide-binding; Reference proteome;
KW   RNA-binding; Transcription; Transferase.
FT   CHAIN           1..465
FT                   /note="Poly(A) polymerase I"
FT                   /id="PRO_0000139095"
FT   REGION          430..465
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        80
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00957"
FT   ACT_SITE        82
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00957"
FT   ACT_SITE        162
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00957"
SQ   SEQUENCE   465 AA;  53922 MW;  9F6C312EDDC8C376 CRC64;
     MFTRVANFCR KVLSREESEA EQAVARPHMT IIPREQHAIS RKDISENALK VLYRLNKAGY
     EAYLVGGGVR DLLLGKKPKD FDVTTNATPD QVRKLFRNCR LVGRRFRLAH VMFGPEIIEV
     ATFRGHHEGS ESDRTTSQRG QNGMLLRDNI FGSIEEDAQR RDFTINSLYY SVADFTVRDY
     VGGMQDLQEG VIRLIGNPET RYREDPVRML RAVRFAAKLN MHISPETAEP IPRLATLLND
     IPPARLFEES LKLLQAGNGY ETYQQLREYH LFQPLFPTIT RYFTENGDSA MERIIAQVLK
     NTDNRIRNEM RVNPAFLFAA MFWYPLLEMA QKIAQESGLA YYDAFALAMN DVLDEACRSL
     AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LMEHPKFRAA FDLLELRAQV ENNTELQRLA
     QWWAEFQASA PPEQKGMLNE LDDDPAPRRR RSRPRKRAPR REGTV