PCNP_MOUSE
ID PCNP_MOUSE Reviewed; 178 AA.
AC Q6P8I4;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=PEST proteolytic signal-containing nuclear protein;
DE Short=PCNP;
DE Short=PEST-containing nuclear protein;
GN Name=Pcnp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=19131326; DOI=10.1074/mcp.m800451-mcp200;
RA Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
RT "Large scale localization of protein phosphorylation by use of electron
RT capture dissociation mass spectrometry.";
RL Mol. Cell. Proteomics 8:904-912(2009).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119 AND THR-139, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-64, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: May be involved in cell cycle regulation. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with UHRF2/NIRF. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- PTM: Ubiquitinated; mediated by UHRF2 and leading to its subsequent
CC proteasomal degradation. {ECO:0000250}.
CC -!- PTM: N-terminally acetylated in a HYPK-dependent manner by the NatA
CC acetyltransferase complex which is composed of NAA10 and NAA15.
CC {ECO:0000250}.
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DR EMBL; BC061235; AAH61235.1; -; mRNA.
DR CCDS; CCDS37357.1; -.
DR RefSeq; NP_001019793.1; NM_001024622.2.
DR AlphaFoldDB; Q6P8I4; -.
DR BioGRID; 218068; 3.
DR STRING; 10090.ENSMUSP00000110087; -.
DR iPTMnet; Q6P8I4; -.
DR PhosphoSitePlus; Q6P8I4; -.
DR EPD; Q6P8I4; -.
DR jPOST; Q6P8I4; -.
DR MaxQB; Q6P8I4; -.
DR PaxDb; Q6P8I4; -.
DR PRIDE; Q6P8I4; -.
DR ProteomicsDB; 288076; -.
DR Antibodypedia; 32287; 176 antibodies from 27 providers.
DR Ensembl; ENSMUST00000114444; ENSMUSP00000110087; ENSMUSG00000071533.
DR GeneID; 76302; -.
DR KEGG; mmu:76302; -.
DR UCSC; uc007zma.1; mouse.
DR CTD; 57092; -.
DR MGI; MGI:1923552; Pcnp.
DR VEuPathDB; HostDB:ENSMUSG00000071533; -.
DR eggNOG; ENOG502QWEZ; Eukaryota.
DR GeneTree; ENSGT00390000010218; -.
DR HOGENOM; CLU_118645_1_0_1; -.
DR InParanoid; Q6P8I4; -.
DR OMA; NVHDQEN; -.
DR OrthoDB; 1616182at2759; -.
DR PhylomeDB; Q6P8I4; -.
DR TreeFam; TF333058; -.
DR BioGRID-ORCS; 76302; 9 hits in 68 CRISPR screens.
DR ChiTaRS; Pcnp; mouse.
DR PRO; PR:Q6P8I4; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q6P8I4; protein.
DR Bgee; ENSMUSG00000071533; Expressed in ureter smooth muscle and 259 other tissues.
DR ExpressionAtlas; Q6P8I4; baseline and differential.
DR Genevisible; Q6P8I4; MM.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:HGNC-UCL.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; ISS:HGNC-UCL.
DR GO; GO:0016567; P:protein ubiquitination; ISS:HGNC-UCL.
DR InterPro; IPR029169; PCNP.
DR PANTHER; PTHR16523; PTHR16523; 1.
DR Pfam; PF15473; PCNP; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell cycle; Nucleus; Phosphoprotein; Reference proteome;
KW Ubl conjugation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q8WW12"
FT CHAIN 2..178
FT /note="PEST proteolytic signal-containing nuclear protein"
FT /id="PRO_0000058254"
FT REGION 1..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 134..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 33..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..63
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 139..154
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q8WW12"
FT MOD_RES 53
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WW12"
FT MOD_RES 64
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 77
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WW12"
FT MOD_RES 87
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WW12"
FT MOD_RES 119
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19131326,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 139
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 147
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8WW12"
FT MOD_RES 150
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8WW12"
FT MOD_RES 152
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8WW12"
SQ SEQUENCE 178 AA; 18963 MW; B1310E3B12BAC309 CRC64;
MADGKAGEEK PEKPQRAGAA GGPEEEAEKP VKTKTVSSSN GGESSSRSAE KRSAEDEAAD
LPTKPTKMSK FGFAIGSQTA RKASAISIRL GASKPKETVP TLAPKTLSVA AAFNEDEDSE
PEEMPPEAKM RMKNIGRDTP TSAGPNSFNK GKHGFSDNQK LWERNIKSHL GNVHDQDN
