PCN_ARATH
ID PCN_ARATH Reviewed; 815 AA.
AC Q8RXU6; Q9S9X3;
DT 28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=WD repeat-containing protein PCN {ECO:0000305};
DE AltName: Full=Protein POPCORN {ECO:0000303|PubMed:22158464};
GN Name=PCN {ECO:0000303|PubMed:22158464};
GN OrderedLocusNames=At4g07410 {ECO:0000312|Araport:AT4G07410};
GN ORFNames=F28D6.14 {ECO:0000312|EMBL:AAD48948.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=22158464; DOI=10.1105/tpc.111.091777;
RA Xiang D., Yang H., Venglat P., Cao Y., Wen R., Ren M., Stone S., Wang E.,
RA Wang H., Xiao W., Weijers D., Berleth T., Laux T., Selvaraj G., Datla R.;
RT "POPCORN functions in the auxin pathway to regulate embryonic body plan and
RT meristem organization in Arabidopsis.";
RL Plant Cell 23:4348-4367(2011).
CC -!- FUNCTION: Involved in auxin signaling pathway. Required for embryo
CC development and meristem organization. Functions in the auxin pathway,
CC integrating auxin signaling in the organization and maintenance of the
CC shoot apical meristem (SAM) and root apical meristem (RAM).
CC {ECO:0000269|PubMed:22158464}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22158464}.
CC -!- DEVELOPMENTAL STAGE: During early phases of embryogenesis, expressed in
CC all the cells of the embryo proper and the suspensor until the 32-cell
CC embryo stage. At the heart stage, expression completely disappears in
CC the suspensor, but remains in the embryo proper. After germination,
CC expressed in shoot apical meristem (SAM), leaf primordia, root apical
CC meristem (RAM), and lateral root primordia.
CC {ECO:0000269|PubMed:22158464}.
CC -!- DISRUPTION PHENOTYPE: Temperature-sensitive phenotype. Defective in
CC embryo development and embryo lethality when grown at 22 degrees
CC Celsius. Embryo lethality phenotype rescued when grown at 29 degrees
CC Celsius. Germination of temperature-rescued pcn mutant seeds give
CC plants with small and narrow-shaped leaves, abnormal leaf vein
CC patterns, altered flowering organs and defects in the root apical
CC meristem (RAM). {ECO:0000269|PubMed:22158464}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD48948.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB81111.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF147262; AAD48948.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161505; CAB81111.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE82567.1; -; Genomic_DNA.
DR EMBL; AY080667; AAL86343.1; -; mRNA.
DR EMBL; AY133764; AAM91698.1; -; mRNA.
DR PIR; C85072; C85072.
DR RefSeq; NP_567317.2; NM_116813.4.
DR AlphaFoldDB; Q8RXU6; -.
DR STRING; 3702.AT4G07410.1; -.
DR iPTMnet; Q8RXU6; -.
DR PaxDb; Q8RXU6; -.
DR PRIDE; Q8RXU6; -.
DR ProteomicsDB; 236713; -.
DR EnsemblPlants; AT4G07410.1; AT4G07410.1; AT4G07410.
DR GeneID; 826172; -.
DR Gramene; AT4G07410.1; AT4G07410.1; AT4G07410.
DR KEGG; ath:AT4G07410; -.
DR Araport; AT4G07410; -.
DR TAIR; locus:2124377; AT4G07410.
DR eggNOG; KOG2048; Eukaryota.
DR HOGENOM; CLU_002392_0_0_1; -.
DR InParanoid; Q8RXU6; -.
DR OMA; PDANWIC; -.
DR OrthoDB; 376282at2759; -.
DR PhylomeDB; Q8RXU6; -.
DR PRO; PR:Q8RXU6; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8RXU6; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0010311; P:lateral root formation; IMP:TAIR.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0010305; P:leaf vascular tissue pattern formation; IMP:TAIR.
DR GO; GO:0035266; P:meristem growth; IMP:TAIR.
DR GO; GO:0010073; P:meristem maintenance; IMP:TAIR.
DR GO; GO:0090696; P:post-embryonic plant organ development; IGI:TAIR.
DR GO; GO:2000012; P:regulation of auxin polar transport; IMP:TAIR.
DR GO; GO:0010449; P:root meristem growth; IMP:TAIR.
DR Gene3D; 2.130.10.10; -; 4.
DR InterPro; IPR044622; PCN.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR45086; PTHR45086; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 8.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..815
FT /note="WD repeat-containing protein PCN"
FT /id="PRO_0000443253"
FT REPEAT 11..50
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 58..103
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 146..190
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 196..235
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 244..284
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 287..326
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 381..421
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 529..568
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT REGION 137..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 141..156
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 815 AA; 90270 MW; C5A1ABCA0CA1AD85 CRC64;
MLEYRCSSVD WKPSPVVALA NSSDDSQVAA AREDGSLEIW LVSPGAVGWH CQLTIHGDPN
SRISSLAWCC SPSIGLPSGR LFSSSIDGSI SEWDLFDLKQ KIVLESIGIS IWQMALAPIS
GFSSDVEGIK NGYLSEKSND EEEIGSEEDG SDSDEFHEKS EEEIDRILAA ACDDGCVRLY
RISNLEKLTY YRSLPRVSGR ALSVTWSPDA KRIFSGSSDG LIRCWDATSC HEVYRITAGL
GGLGSSSEIC VWSLLSLRCS VLVSGDSTGT VQFWDSEHGT LLEAHSNHKG DVNTLAAAPS
HNRVFSAGAD GQVILYKLSG STNGSQDLKP SSSQKWDYIG YVKAHTHDIR ALTVAVPISR
EDPFPDDILP DKASRKHRKK GKPVDFTYHK WAHLGVPMLI SAGDDAKLFA YSIQEFTKFS
PHDICPAPQR IPMQMVHNSM FNKTSLLLVQ GISTLDILRL NISSDSSGRA STKSLVRVKS
RDARKIICSA ISNTGSHFAY SDQIGPSLFE LKKNEFTKCP WSVSRRRLPE LPFAHSMIFS
SDCSRLIIAG HDRRIYTIDI SSLELVYAFT PSREEHEGEA PTPKEPPITK LFTSSDGQWL
AAINCFGDIY VFNLETQRQH WFISRLDGAS VTAAGFHPWN NNALVISTSS NQVFAFDVEA
RQLGKWSLLN TYVLPKRYQE FPGEVLGLSF SPSPNSSSVI VYSSRAKCLI DFGKPVEEDE
EYDLPNGNLS KTLEGKLVNL GLKKGKGTNR KRRLDEYQLE GKSNERKNFE ILPSNHPVLF
VGHLSKNSIL VIEKPWMDVV KSLDNQPVDR HIFGT
