ID   PCOS_ECOLX              Reviewed;         466 AA.
AC   Q47457;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Probable sensor protein PcoS;
DE            EC=2.7.13.3;
GN   Name=pcoS;
OS   Escherichia coli.
OG   Plasmid pRJ1004.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RJ97;
RX   PubMed=8594334; DOI=10.1111/j.1365-2958.1995.mmi_17061153.x;
RA   Brown N.L., Barrett S.R., Camakaris J., Lee B.T.O., Rouch D.A.;
RT   "Molecular genetics and transport analysis of the copper-resistance
RT   determinant (pco) from Escherichia coli plasmid pRJ1004.";
RL   Mol. Microbiol. 17:1153-1166(1995).
CC   -!- FUNCTION: Probable member of a two-component regulatory system
CC       PcoS/PcoR. May activate PcoR by phosphorylation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
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DR   EMBL; X83541; CAA58530.1; -; Genomic_DNA.
DR   PIR; S70165; S52258.
DR   AlphaFoldDB; Q47457; -.
DR   SMR; Q47457; -.
DR   STRING; 481805.EcolC_3411; -.
DR   eggNOG; COG2205; Bacteria.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR006290; CztS_silS_copS.
DR   InterPro; IPR003660; HAMP_dom.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF00672; HAMP; 1.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00304; HAMP; 1.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   TIGRFAMs; TIGR01386; cztS_silS_copS; 1.
DR   PROSITE; PS50885; HAMP; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell inner membrane; Cell membrane; Copper; Kinase; Membrane;
KW   Nucleotide-binding; Phosphoprotein; Plasmid; Transferase; Transmembrane;
KW   Transmembrane helix; Two-component regulatory system.
FT   CHAIN           1..466
FT                   /note="Probable sensor protein PcoS"
FT                   /id="PRO_0000074738"
FT   TOPO_DOM        1..10
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        32..171
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        193..466
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          193..246
FT                   /note="HAMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT   DOMAIN          254..466
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         257
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   466 AA;  53071 MW;  E352DA64FADA5A8E CRC64;
     MRFKISLTTR LSLIFSAVML TVWWLSSFIL ISTLNDYFDN QDRDFLTGKL QLTEEFLKTE
     TFRNKTDIKS LSEKINDAMV GHNGLFISIK NMENEKIVEL YAKNSVVPAV LLNKSGDILD
     YMIQTEENNT VYRSISRRVA VTPEQGKSKH VIITVATDTG YHTLLMDKLS TWLFWFNIGL
     VFISVFLGWL TTRIGLKPLR EMTSLASSMT VHSLDQRLNP DLAPPEISET MQEFNNMFDR
     LEGAFRKLSD FSSDIAHELR TPVSNLMMQT QFALAKERDV SHYREILFAN LEELKRLSRM
     TSDMLFLARS EHGLLRLDKH DVDLAAELNE LRELFEPLAD ETGKTITVEG EGVVAGDSDM
     LRRAFSNLLS NAIKYSPDNT CTAIHLERDS DCVNVMITNT MSGQVPANLE RLFDRFYRAD
     SSRFYNTEGA GLGLSITRSI IHAHGGELSA EQQGREIVFK VRLLMD