PCP_BACAN
ID PCP_BACAN Reviewed; 215 AA.
AC Q81NT5; Q6HX05;
DT 13-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Pyrrolidone-carboxylate peptidase {ECO:0000255|HAMAP-Rule:MF_00417};
DE EC=3.4.19.3 {ECO:0000255|HAMAP-Rule:MF_00417};
DE AltName: Full=5-oxoprolyl-peptidase {ECO:0000255|HAMAP-Rule:MF_00417};
DE AltName: Full=Pyroglutamyl-peptidase I {ECO:0000255|HAMAP-Rule:MF_00417};
DE Short=PGP-I {ECO:0000255|HAMAP-Rule:MF_00417};
DE Short=Pyrase {ECO:0000255|HAMAP-Rule:MF_00417};
GN Name=pcp {ECO:0000255|HAMAP-Rule:MF_00417};
GN OrderedLocusNames=BA_3090, GBAA_3090, BAS2875;
OS Bacillus anthracis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1392;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames / isolate Porton;
RX PubMed=12721629; DOI=10.1038/nature01586;
RA Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT related bacteria.";
RL Nature 423:81-86(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sterne;
RA Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA Richardson P., Rubin E., Tice H.;
RT "Complete genome sequence of Bacillus anthracis Sterne.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RX PubMed=18952800; DOI=10.1128/jb.01347-08;
RA Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL J. Bacteriol. 191:445-446(2009).
CC -!- FUNCTION: Removes 5-oxoproline from various penultimate amino acid
CC residues except L-proline. {ECO:0000255|HAMAP-Rule:MF_00417}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of an N-terminal pyroglutamyl group from a
CC polypeptide, the second amino acid generally not being Pro.;
CC EC=3.4.19.3; Evidence={ECO:0000255|HAMAP-Rule:MF_00417};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00417}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00417}.
CC -!- SIMILARITY: Belongs to the peptidase C15 family. {ECO:0000255|HAMAP-
CC Rule:MF_00417}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE016879; AAP26903.1; -; Genomic_DNA.
DR EMBL; AE017225; AAT55184.1; -; Genomic_DNA.
DR EMBL; AE017334; AAT70140.1; -; Genomic_DNA.
DR RefSeq; NP_845417.1; NC_003997.3.
DR RefSeq; WP_000859733.1; NZ_WXXJ01000001.1.
DR RefSeq; YP_029133.1; NC_005945.1.
DR PDB; 3LAC; X-ray; 2.00 A; A/B=1-215.
DR PDBsum; 3LAC; -.
DR AlphaFoldDB; Q81NT5; -.
DR SMR; Q81NT5; -.
DR STRING; 260799.BAS2875; -.
DR MEROPS; C15.001; -.
DR DNASU; 1086349; -.
DR EnsemblBacteria; AAP26903; AAP26903; BA_3090.
DR EnsemblBacteria; AAT70140; AAT70140; GBAA_3090.
DR GeneID; 45022894; -.
DR KEGG; ban:BA_3090; -.
DR KEGG; bar:GBAA_3090; -.
DR KEGG; bat:BAS2875; -.
DR PATRIC; fig|198094.11.peg.3072; -.
DR eggNOG; COG2039; Bacteria.
DR HOGENOM; CLU_043960_4_0_9; -.
DR OMA; KLAYNHK; -.
DR EvolutionaryTrace; Q81NT5; -.
DR Proteomes; UP000000427; Chromosome.
DR Proteomes; UP000000594; Chromosome.
DR GO; GO:0005829; C:cytosol; IEA:InterPro.
DR GO; GO:0016920; F:pyroglutamyl-peptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00501; Peptidase_C15; 1.
DR Gene3D; 3.40.630.20; -; 1.
DR HAMAP; MF_00417; Pyrrolid_peptidase; 1.
DR InterPro; IPR000816; Peptidase_C15.
DR InterPro; IPR016125; Peptidase_C15-like.
DR InterPro; IPR036440; Peptidase_C15-like_sf.
DR InterPro; IPR029762; PGP-I_bact-type.
DR InterPro; IPR033694; PGPEP1_Cys_AS.
DR InterPro; IPR033693; PGPEP1_Glu_AS.
DR PANTHER; PTHR23402; PTHR23402; 1.
DR Pfam; PF01470; Peptidase_C15; 1.
DR PIRSF; PIRSF015592; Prld-crbxl_pptds; 1.
DR PRINTS; PR00706; PYROGLUPTASE.
DR SUPFAM; SSF53182; SSF53182; 1.
DR TIGRFAMs; TIGR00504; pyro_pdase; 1.
DR PROSITE; PS01334; PYRASE_CYS; 1.
DR PROSITE; PS01333; PYRASE_GLU; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Protease; Reference proteome;
KW Thiol protease.
FT CHAIN 1..215
FT /note="Pyrrolidone-carboxylate peptidase"
FT /id="PRO_0000184707"
FT ACT_SITE 80
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00417"
FT ACT_SITE 143
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00417"
FT ACT_SITE 167
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00417"
FT STRAND 2..9
FT /evidence="ECO:0007829|PDB:3LAC"
FT HELIX 19..25
FT /evidence="ECO:0007829|PDB:3LAC"
FT TURN 26..29
FT /evidence="ECO:0007829|PDB:3LAC"
FT STRAND 35..42
FT /evidence="ECO:0007829|PDB:3LAC"
FT HELIX 48..60
FT /evidence="ECO:0007829|PDB:3LAC"
FT STRAND 63..70
FT /evidence="ECO:0007829|PDB:3LAC"
FT STRAND 75..80
FT /evidence="ECO:0007829|PDB:3LAC"
FT STRAND 82..85
FT /evidence="ECO:0007829|PDB:3LAC"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:3LAC"
FT STRAND 110..113
FT /evidence="ECO:0007829|PDB:3LAC"
FT HELIX 118..127
FT /evidence="ECO:0007829|PDB:3LAC"
FT STRAND 132..136
FT /evidence="ECO:0007829|PDB:3LAC"
FT HELIX 142..154
FT /evidence="ECO:0007829|PDB:3LAC"
FT HELIX 155..157
FT /evidence="ECO:0007829|PDB:3LAC"
FT STRAND 160..168
FT /evidence="ECO:0007829|PDB:3LAC"
FT HELIX 172..175
FT /evidence="ECO:0007829|PDB:3LAC"
FT HELIX 186..200
FT /evidence="ECO:0007829|PDB:3LAC"
SQ SEQUENCE 215 AA; 23513 MW; 352437CBE86717A6 CRC64;
MKTVLLTGFD PFGGESINPA WEVAKSLHEK TIGEYKIISK QVPTVFHKSI SVLKEYIEEL
APEFIICIGQ AGGRPDITIE RVAINIDDAR IADNEGNQPV DVPVVEEGPA AYWSTLPMKA
IVKKLQEEGI PASVSQTAGT FVCNHLFYGL MHELEKHDTK MKGGFIHIPF LPEQASNYPG
QPSMSLSTIR KGIELAVEVT TTVEVDIVEV GGTTH
