ID   PCP_DICDI               Reviewed;         406 AA.
AC   Q9GPM2; Q54C17;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Paracaspase;
DE            EC=3.4.22.-;
GN   Name=pcp; ORFNames=DDB_G0293196;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11090634; DOI=10.1016/s1097-2765(00)00094-0;
RA   Uren A.G., O'Rourke K., Aravind L., Pisabarro M.T., Seshagiri S.,
RA   Koonin E.V., Dixit V.M.;
RT   "Identification of paracaspases and metacaspases. Two ancient families of
RT   caspase-like proteins, one of which plays a key role in MALT lymphoma.";
RL   Mol. Cell 6:961-967(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=14681218; DOI=10.1074/jbc.m312741200;
RA   Roisin-Bouffay C., Luciani M.-F., Klein G., Levraud J.-P., Adam M.,
RA   Golstein P.;
RT   "Developmental cell death in dictyostelium does not require paracaspase.";
RL   J. Biol. Chem. 279:11489-11494(2004).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=17889831; DOI=10.1016/j.bbrc.2007.09.011;
RA   Lam D., Levraud J.-P., Luciani M.-F., Golstein P.;
RT   "Autophagic or necrotic cell death in the absence of caspase and bcl-2
RT   family members.";
RL   Biochem. Biophys. Res. Commun. 363:536-541(2007).
CC   -!- FUNCTION: Not required for DIF-induced autophagic cell death and
CC       necrotic cell death. {ECO:0000269|PubMed:14681218,
CC       ECO:0000269|PubMed:17889831}.
CC   -!- DISRUPTION PHENOTYPE: No change in morphology and persistence of
CC       induced-cell death. {ECO:0000269|PubMed:14681218,
CC       ECO:0000269|PubMed:17889831}.
CC   -!- SIMILARITY: Belongs to the peptidase C14B family. {ECO:0000305}.
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DR   EMBL; AF316600; AAG38592.1; -; mRNA.
DR   EMBL; AAFI02000200; EAL60797.1; -; Genomic_DNA.
DR   RefSeq; XP_629249.1; XM_629247.1.
DR   AlphaFoldDB; Q9GPM2; -.
DR   SMR; Q9GPM2; -.
DR   STRING; 44689.DDB0215375; -.
DR   MEROPS; C14.A07; -.
DR   PaxDb; Q9GPM2; -.
DR   EnsemblProtists; EAL60797; EAL60797; DDB_G0293196.
DR   GeneID; 8629131; -.
DR   KEGG; ddi:DDB_G0293196; -.
DR   dictyBase; DDB_G0293196; pcp.
DR   HOGENOM; CLU_678684_0_0_1; -.
DR   InParanoid; Q9GPM2; -.
DR   PRO; PR:Q9GPM2; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0031164; C:contractile vacuolar membrane; IDA:dictyBase.
DR   GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0033298; P:contractile vacuole organization; IMP:dictyBase.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0006970; P:response to osmotic stress; IMP:dictyBase.
DR   InterPro; IPR029030; Caspase-like_dom_sf.
DR   SUPFAM; SSF52129; SSF52129; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Protease; Reference proteome.
FT   CHAIN           1..406
FT                   /note="Paracaspase"
FT                   /id="PRO_0000384450"
FT   REGION          193..374
FT                   /note="Caspase-like"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        266
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        311
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   406 AA;  46537 MW;  E2AE4A25C5D86AD7 CRC64;
     MSETFIHSVS ENQIIVNDLE NNNDYDNEDN VFIDSSVYNQ ECFMEILKKL EDGKPIHEVV
     RFTSHSSYTH TIVKTIQGLF LYVVLKSVNI YGSYYDFQRY IGPSMMIGTY ACPLYRVQYR
     INKNDSTGAI IKYSNNLDEC ETYFNHLCKV FNVLTKRLSL NSICCGVGLS DSFYLMNTKT
     IKKSSRTRIS FIIGNSKYSQ HRKLDGVIND VNSFYCALLG CSFHSDNIVW LIDSDLRTFY
     DKWYTFLQLV QSFQSYIEVV VYYAGHGRSD NGNLKLIMTD GNPVQLSIIA STLTESIKNS
     DSLCLFIVDC CRDGENVLPF HYPIESFDDK KNIAVLFACK YGELSYESLE LNAGIFTRSF
     VKTITERKNN NISTICNLTD IAIKRIYKNY SGCSLIGNFD CSLLEF