PCR1_ARATH
ID PCR1_ARATH Reviewed; 151 AA.
AC Q9LQU2;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Protein PLANT CADMIUM RESISTANCE 1;
DE Short=AtPCR1;
GN Name=PCR1; OrderedLocusNames=At1g14880; ORFNames=F10B6.29;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, MUTAGENESIS OF CYS-29; CYS-30; CYS-35; PRO-36 AND CYS-37, TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15181212; DOI=10.1104/pp.103.037739;
RA Song W.Y., Martinoia E., Lee J., Kim D., Kim D.Y., Vogt E., Shim D.,
RA Choi K.S., Hwang I., Lee Y.;
RT "A novel family of cys-rich membrane proteins mediates cadmium resistance
RT in Arabidopsis.";
RL Plant Physiol. 135:1027-1039(2004).
RN [6]
RP 3D-STRUCTURE MODELING.
RX PubMed=20400678; DOI=10.1105/tpc.109.073676;
RA Guo M., Rupe M.A., Dieter J.A., Zou J., Spielbauer D., Duncan K.E.,
RA Howard R.J., Hou Z., Simmons C.R.;
RT "Cell Number Regulator1 affects plant and organ size in maize: implications
RT for crop yield enhancement and heterosis.";
RL Plant Cell 22:1057-1073(2010).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=20647347; DOI=10.1105/tpc.109.070185;
RA Song W.Y., Choi K.S., Kim do Y., Geisler M., Park J., Vincenzetti V.,
RA Schellenberg M., Kim S.H., Lim Y.P., Noh E.W., Lee Y., Martinoia E.;
RT "Arabidopsis PCR2 is a zinc exporter involved in both zinc extrusion and
RT long-distance zinc transport.";
RL Plant Cell 22:2237-2252(2010).
CC -!- FUNCTION: Involved in glutathione-independent cadmium resistance.
CC Reduces cadmium uptake rather than activating efflux, but is not
CC closely coupled to calcium transporter. {ECO:0000269|PubMed:15181212,
CC ECO:0000269|PubMed:20647347}.
CC -!- SUBUNIT: Homopentamer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in aerial part, but not in roots.
CC Detected in the guard and mesophyll cells.
CC {ECO:0000269|PubMed:15181212, ECO:0000269|PubMed:20647347}.
CC -!- INDUCTION: Up-regulated in shoots by cadmium.
CC -!- DOMAIN: the N-terminal domain (1-84) is required for cadmium
CC resistance.
CC -!- DISRUPTION PHENOTYPE: No heavy metal-related phenotype.
CC {ECO:0000269|PubMed:20647347}.
CC -!- SIMILARITY: Belongs to the cornifelin family. {ECO:0000305}.
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DR EMBL; AC006917; AAF79233.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29238.1; -; Genomic_DNA.
DR EMBL; AK118861; BAC43448.1; -; mRNA.
DR EMBL; BT005663; AAO64083.1; -; mRNA.
DR RefSeq; NP_172941.1; NM_101357.4.
DR AlphaFoldDB; Q9LQU2; -.
DR STRING; 3702.AT1G14880.1; -.
DR TCDB; 1.A.87.3.7; the mechanosensitive calcium channel (mca) family.
DR PaxDb; Q9LQU2; -.
DR PRIDE; Q9LQU2; -.
DR ProteomicsDB; 236443; -.
DR EnsemblPlants; AT1G14880.1; AT1G14880.1; AT1G14880.
DR GeneID; 838053; -.
DR Gramene; AT1G14880.1; AT1G14880.1; AT1G14880.
DR KEGG; ath:AT1G14880; -.
DR Araport; AT1G14880; -.
DR TAIR; locus:2006767; AT1G14880.
DR eggNOG; ENOG502S7UD; Eukaryota.
DR HOGENOM; CLU_083147_1_0_1; -.
DR OMA; EMGTCCC; -.
DR OrthoDB; 1354725at2759; -.
DR PhylomeDB; Q9LQU2; -.
DR PRO; PR:Q9LQU2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LQU2; baseline and differential.
DR Genevisible; Q9LQU2; AT.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR InterPro; IPR006461; PLAC_motif_containing.
DR PANTHER; PTHR15907; PTHR15907; 1.
DR Pfam; PF04749; PLAC8; 1.
DR TIGRFAMs; TIGR01571; A_thal_Cys_rich; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..151
FT /note="Protein PLANT CADMIUM RESISTANCE 1"
FT /id="PRO_0000407718"
FT TRANSMEM 31..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 29
FT /note="C->A: No effect on cadmium resistance. Total loss of
FT cadmium resistance in a heterologous system; when
FT associated with A-30; A-35; A-36 and A-37."
FT /evidence="ECO:0000269|PubMed:15181212"
FT MUTAGEN 30
FT /note="C->A: No effect on cadmium resistance. Total loss of
FT cadmium resistance in a heterologous system; when
FT associated with A-29; A-35; A-36 and A-37."
FT /evidence="ECO:0000269|PubMed:15181212"
FT MUTAGEN 35
FT /note="C->A: No effect on cadmium resistance. Decreased
FT cadmium resistance in a heterologous system; when
FT associated with A-36 and A-37. Total loss of cadmium
FT resistance in a heterologous system; when associated with
FT A-29; A-30; A-36 and A-37."
FT /evidence="ECO:0000269|PubMed:15181212"
FT MUTAGEN 36
FT /note="P->A: No effect on cadmium resistance. Decreased
FT cadmium resistance in a heterologous system; when
FT associated with A-35 and A-37. Total loss of cadmium
FT resistance in a heterologous system; when associated with
FT A-29; A-30; A-35 and A-37."
FT /evidence="ECO:0000269|PubMed:15181212"
FT MUTAGEN 37
FT /note="C->A: No effect on cadmium resistance. Decreased
FT cadmium resistance in a heterologous system; when
FT associated with A-35 and A-36. Total loss of cadmium
FT resistance in a heterologous system; when associated with
FT A-29; A-30; A-35 and A-36."
FT /evidence="ECO:0000269|PubMed:15181212"
SQ SEQUENCE 151 AA; 16503 MW; 7D17F5B8CA5E9368 CRC64;
MEAQLHAKPH AQGEWSTGFC DCFSDCRNCC ITLCCPCITF GQVAEIVDRG SKSCCAAGAL
YMLIDLITSC GRMYACFYSG KMRAQYNIKG DGCTDCLKHF CCNLCALTQQ YRELKHRGFD
MSLGWAGNAE KQQNQGGVAM GAPAFQGGMT R
