PCR2_ARATH
ID PCR2_ARATH Reviewed; 152 AA.
AC Q9LQU4;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Protein PLANT CADMIUM RESISTANCE 2;
DE Short=AtPCR2;
GN Name=PCR2; OrderedLocusNames=At1g14870; ORFNames=F10B6.27;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION BY CADMIUM, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=15181212; DOI=10.1104/pp.103.037739;
RA Song W.Y., Martinoia E., Lee J., Kim D., Kim D.Y., Vogt E., Shim D.,
RA Choi K.S., Hwang I., Lee Y.;
RT "A novel family of cys-rich membrane proteins mediates cadmium resistance
RT in Arabidopsis.";
RL Plant Physiol. 135:1027-1039(2004).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY ZINC,
RP SUBUNIT, AND DISRUPTION PHENOTYPE.
RX PubMed=20647347; DOI=10.1105/tpc.109.070185;
RA Song W.Y., Choi K.S., Kim do Y., Geisler M., Park J., Vincenzetti V.,
RA Schellenberg M., Kim S.H., Lim Y.P., Noh E.W., Lee Y., Martinoia E.;
RT "Arabidopsis PCR2 is a zinc exporter involved in both zinc extrusion and
RT long-distance zinc transport.";
RL Plant Cell 22:2237-2252(2010).
CC -!- FUNCTION: Zinc transporter acting in both zinc extrusion and long-
CC distance zinc transport. Involved in the loading of zinc into the
CC xyleme and in the detoxification of excess zinc at the epidermal cells.
CC Acts independently from the zinc transporters HMA2 and HMA4. May be
CC also involved in cadmium resistance. {ECO:0000269|PubMed:15181212,
CC ECO:0000269|PubMed:20647347}.
CC -!- SUBUNIT: Homooligomer. {ECO:0000269|PubMed:20647347}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20647347};
CC Single-pass membrane protein {ECO:0000269|PubMed:20647347}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, shoots, stems, flowers
CC and siliques. In leaves, restricted mainly to the vascular tissue.
CC Expressed in all cells in the root tip, in the vascular tissue and the
CC epidermis in the elongation zone, and only in the epidermal cells in
CC the root hair zone. {ECO:0000269|PubMed:15181212,
CC ECO:0000269|PubMed:20647347}.
CC -!- INDUCTION: Constitutively expressed. Up-regulated in the shoots by
CC cadmium. Down-regulated under zinc deficiency.
CC {ECO:0000269|PubMed:15181212, ECO:0000269|PubMed:20647347}.
CC -!- DISRUPTION PHENOTYPE: Strongly impaired growth in the presence of heavy
CC metals such as zinc, cadmium, copper or iron.
CC {ECO:0000269|PubMed:20647347}.
CC -!- SIMILARITY: Belongs to the cornifelin family. {ECO:0000305}.
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DR EMBL; AC006917; AAF79235.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29237.1; -; Genomic_DNA.
DR EMBL; AY072397; AAL62389.1; -; mRNA.
DR EMBL; AY114701; AAM48020.1; -; mRNA.
DR RefSeq; NP_172940.1; NM_101356.5.
DR AlphaFoldDB; Q9LQU4; -.
DR BioGRID; 23292; 4.
DR IntAct; Q9LQU4; 1.
DR STRING; 3702.AT1G14870.1; -.
DR TCDB; 1.A.87.3.8; the mechanosensitive calcium channel (mca) family.
DR SwissPalm; Q9LQU4; -.
DR PaxDb; Q9LQU4; -.
DR PRIDE; Q9LQU4; -.
DR ProteomicsDB; 234949; -.
DR EnsemblPlants; AT1G14870.1; AT1G14870.1; AT1G14870.
DR GeneID; 838052; -.
DR Gramene; AT1G14870.1; AT1G14870.1; AT1G14870.
DR KEGG; ath:AT1G14870; -.
DR Araport; AT1G14870; -.
DR TAIR; locus:2006772; AT1G14870.
DR eggNOG; ENOG502S7UD; Eukaryota.
DR HOGENOM; CLU_083147_1_0_1; -.
DR InParanoid; Q9LQU4; -.
DR OMA; NCCITLW; -.
DR OrthoDB; 1354725at2759; -.
DR PhylomeDB; Q9LQU4; -.
DR PRO; PR:Q9LQU4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LQU4; baseline and differential.
DR Genevisible; Q9LQU4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0006979; P:response to oxidative stress; IMP:TAIR.
DR InterPro; IPR006461; PLAC_motif_containing.
DR PANTHER; PTHR15907; PTHR15907; 1.
DR Pfam; PF04749; PLAC8; 1.
DR TIGRFAMs; TIGR01571; A_thal_Cys_rich; 1.
DR PROSITE; PS00010; ASX_HYDROXYL; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..152
FT /note="Protein PLANT CADMIUM RESISTANCE 2"
FT /id="PRO_0000300105"
FT TRANSMEM 57..79
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 152 AA; 16742 MW; BC0655E747D315D1 CRC64;
MEAQHLHAKP HAEGEWSTGF CDCFSDCKNC CITFWCPCIT FGQVAEIVDR GSTSCGTAGA
LYALIAVVTG CACIYSCFYR GKMRAQYNIK GDDCTDCLKH FCCELCSLTQ QYRELKHRGY
DMSLGWAGNV ERQQNQGGVA MGAPVFQGGM TR
