PCR8_ARATH
ID PCR8_ARATH Reviewed; 190 AA.
AC Q9M815; Q8LE32;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Protein PLANT CADMIUM RESISTANCE 8;
DE Short=AtPCR8;
GN Name=PCR8; OrderedLocusNames=At1g52200; ORFNames=F9I5.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15181212; DOI=10.1104/pp.103.037739;
RA Song W.Y., Martinoia E., Lee J., Kim D., Kim D.Y., Vogt E., Shim D.,
RA Choi K.S., Hwang I., Lee Y.;
RT "A novel family of cys-rich membrane proteins mediates cadmium resistance
RT in Arabidopsis.";
RL Plant Physiol. 135:1027-1039(2004).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=19334764; DOI=10.1021/pr801044y;
RA Mitra S.K., Walters B.T., Clouse S.D., Goshe M.B.;
RT "An efficient organic solvent based extraction method for the proteomic
RT analysis of Arabidopsis plasma membranes.";
RL J. Proteome Res. 8:2752-2767(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-23, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: May be involved in heavy metals transport. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19334764};
CC Single-pass membrane protein {ECO:0000269|PubMed:19334764}.
CC -!- SIMILARITY: Belongs to the cornifelin family. {ECO:0000305}.
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DR EMBL; AC022354; AAF29406.2; -; Genomic_DNA.
DR EMBL; CP002684; AEE32768.1; -; Genomic_DNA.
DR EMBL; BT004773; AAO44039.1; -; mRNA.
DR EMBL; AY085651; AAM62872.1; -; mRNA.
DR EMBL; AK227386; BAE99392.1; -; mRNA.
DR PIR; A96562; A96562.
DR RefSeq; NP_564602.1; NM_104100.5.
DR AlphaFoldDB; Q9M815; -.
DR BioGRID; 26875; 2.
DR STRING; 3702.AT1G52200.1; -.
DR iPTMnet; Q9M815; -.
DR PaxDb; Q9M815; -.
DR PRIDE; Q9M815; -.
DR ProteomicsDB; 236666; -.
DR EnsemblPlants; AT1G52200.1; AT1G52200.1; AT1G52200.
DR GeneID; 841650; -.
DR Gramene; AT1G52200.1; AT1G52200.1; AT1G52200.
DR KEGG; ath:AT1G52200; -.
DR Araport; AT1G52200; -.
DR TAIR; locus:2037458; AT1G52200.
DR eggNOG; ENOG502RZFT; Eukaryota.
DR HOGENOM; CLU_083147_1_1_1; -.
DR InParanoid; Q9M815; -.
DR OMA; HENQTNA; -.
DR OrthoDB; 1354725at2759; -.
DR PhylomeDB; Q9M815; -.
DR PRO; PR:Q9M815; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9M815; baseline and differential.
DR Genevisible; Q9M815; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006979; P:response to oxidative stress; IMP:TAIR.
DR InterPro; IPR006461; PLAC_motif_containing.
DR PANTHER; PTHR15907; PTHR15907; 1.
DR Pfam; PF04749; PLAC8; 1.
DR TIGRFAMs; TIGR01571; A_thal_Cys_rich; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..190
FT /note="Protein PLANT CADMIUM RESISTANCE 8"
FT /id="PRO_0000407724"
FT TRANSMEM 94..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 23
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT CONFLICT 176
FT /note="R -> S (in Ref. 4; AAM62872)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 190 AA; 21179 MW; 9708EF167B0031C0 CRC64;
MGRVTTPSEE DSNNGLPVQQ PGTPNQRTRV PVSQFAPPNY QQANVNLSVG RPWSTGLFDC
QADQANAVLT TIVPCVTFGQ IAEVMDEGEM TCPLGTFMYL LMMPALCSHW VMGSKYREKM
RRKFNLVEAP YSDCASHVLC PCCSLCQEYR ELKIRNLDPS LGWNGILAQG QGQYEREAPS
FAPTNQYMSK
