ID   PCRA_BACSU              Reviewed;         739 AA.
AC   O34580;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 141.
DE   RecName: Full=ATP-dependent DNA helicase PcrA;
DE            EC=3.6.4.12;
GN   Name=pcrA; Synonyms=yerF; OrderedLocusNames=BSU06610;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9701819; DOI=10.1046/j.1365-2958.1998.00927.x;
RA   Petit M.-A., Dervyn E., Rose M., Entian K.-D., McGovern S., Ehrlich S.D.,
RA   Bruand C.;
RT   "PcrA is an essential DNA helicase of Bacillus subtilis fulfilling
RT   functions both in repair and rolling-circle replication.";
RL   Mol. Microbiol. 29:261-273(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   INTERACTION WITH YXAL; YWHK AND YERB.
RX   PubMed=12073041; DOI=10.1007/s00438-002-0670-9;
RA   Noirot-Gros M.-F., Soultanas P., Wigley D.B., Ehrlich S.D., Noirot P.,
RA   Petit M.-A.;
RT   "The beta-propeller protein YxaL increases the processivity of the PcrA
RT   helicase.";
RL   Mol. Genet. Genomics 267:391-400(2002).
RN   [4]
RP   SUBUNIT.
RC   STRAIN=168;
RX   PubMed=21710567; DOI=10.1002/pmic.201000790;
RA   Delumeau O., Lecointe F., Muntel J., Guillot A., Guedon E., Monnet V.,
RA   Hecker M., Becher D., Polard P., Noirot P.;
RT   "The dynamic protein partnership of RNA polymerase in Bacillus subtilis.";
RL   Proteomics 11:2992-3001(2011).
CC   -!- FUNCTION: DNA helicase used for plasmid rolling-circle replication and
CC       also involved in UV repair.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Interacts with YxaL; YwhK and YerB. Interacts with the RNA
CC       polymerase core (PubMed:21710567). {ECO:0000269|PubMed:12073041,
CC       ECO:0000269|PubMed:21710567}.
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
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DR   EMBL; Y15254; CAA75552.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12481.1; -; Genomic_DNA.
DR   PIR; E69794; E69794.
DR   RefSeq; NP_388543.1; NC_000964.3.
DR   RefSeq; WP_003233919.1; NZ_JNCM01000032.1.
DR   AlphaFoldDB; O34580; -.
DR   SMR; O34580; -.
DR   IntAct; O34580; 5.
DR   STRING; 224308.BSU06610; -.
DR   PaxDb; O34580; -.
DR   EnsemblBacteria; CAB12481; CAB12481; BSU_06610.
DR   GeneID; 938747; -.
DR   KEGG; bsu:BSU06610; -.
DR   PATRIC; fig|224308.179.peg.719; -.
DR   eggNOG; COG0210; Bacteria.
DR   InParanoid; O34580; -.
DR   OMA; YQDTNRT; -.
DR   PhylomeDB; O34580; -.
DR   BioCyc; BSUB:BSU06610-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0033202; C:DNA helicase complex; IBA:GO_Central.
DR   GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR   GO; GO:0000725; P:recombinational repair; IBA:GO_Central.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01073; pcrA; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..739
FT                   /note="ATP-dependent DNA helicase PcrA"
FT                   /id="PRO_0000102051"
FT   DOMAIN          10..289
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT   DOMAIN          290..570
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00617"
FT   REGION          655..678
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         34..39
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT   BINDING         287
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   739 AA;  83555 MW;  70C553EFC88E6D6D CRC64;
     MNYISNQLLS GLNPVQQEAV KTTDGPLLLM AGAGSGKTRV LTHRIAYLMA EKHVAPWNIL
     AITFTNKAAR EMKERVESIL GPGADDIWIS TFHSMCVRIL RRDIDRIGIN RNFSILDTAD
     QLSVIKGILK ERNLDPKKFD PRSILGTISS AKNELTEPEE FSKVAGGYYD QVVSDVYADY
     QKKLLKNQSL DFDDLIMTTI KLFDRVPEVL EFYQRKFQYI HVDEYQDTNR AQYMLVKQLA
     ERFQNLCVVG DSDQSIYRWR GADITNILSF EKDYPNASVI LLEQNYRSTK RILRAANEVI
     KNNSNRKPKN LWTENDEGIK ISYYRGDNEF GEGQFVAGKI HQLHSTGKRK LSDIAILYRT
     NAQSRVIEET LLKAGLNYNI VGGTKFYDRK EIKDILAYLR LVSNPDDDIS FTRIVNVPKR
     GVGATSLEKI ASYAAINGLS FFQAIQQVDF IGVSAKAANA LDSFRQMIEN LTNMQDYLSI
     TELTEEILDK TEYREMLKAE KSIEAQSRLE NIDEFLSVTK NFEQKSEDKT LVAFLTDLAL
     IADIDQLDQK EEESGGKDAI TLMTLHAAKG LEFPVVFLMG LEEGVFPHSR SLMEEAEMEE
     ERRLAYVGIT RAEQELYLTN AKMRTLFGRT NMNPESRFIA EIPDDLLENL NEKKETRATS
     ARKMQPRRGP VSRPVSYASK TGGDTLNWAV GDKAGHKKWG TGTVVSVKGE GEGTELDIAF
     PSPVGVKRLL AAFAPIEKQ