PCRA_LEUCI
ID PCRA_LEUCI Reviewed; 749 AA.
AC Q9S3Q0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=ATP-dependent DNA helicase PcrA;
DE EC=3.6.4.12;
GN Name=pcrA;
OS Leuconostoc citreum.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Leuconostoc.
OX NCBI_TaxID=33964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CNRZ 1478 / 22R;
RA Cibik R., Quiberoni A., Tailliez P.;
RT "Cloning and sequencing of the DNA helicase gene of Leuconostoc citreum.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Essential helicase.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF176554; AAD51119.1; -; Genomic_DNA.
DR PIR; T44796; T44796.
DR AlphaFoldDB; Q9S3Q0; -.
DR SMR; Q9S3Q0; -.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01073; pcrA; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW Nucleotide-binding.
FT CHAIN 1..749
FT /note="ATP-dependent DNA helicase PcrA"
FT /id="PRO_0000102052"
FT DOMAIN 8..288
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT DOMAIN 289..570
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00617"
FT BINDING 32..37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT BINDING 286
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 749 AA; 84442 MW; 8A753487DE7B73A3 CRC64;
MSVETLTNGM NNKQAEAVQT TEGPLLIMAG AGSGKTRVLT HRIAHLVQDL NVFPWRILAI
TFTNKAAREM RERIAALLSE DVARDIWVST FHALAVRILR RDGEAIGLAK NFTIIDTSAQ
RTLMKRVIND LNLDTNQYDP RTILGMISNA KNDMLQPRDY AKAADNAFQE TVAEVYTAYQ
AELKRSQSVD FDDLIMLTID LFQSAPDVLA RYQQQFEYLH VDEYQDTNDA QYTIVNLLAQ
RSKNLAVVGD ADQSIYGWRG ANMNNILNFE KDYPNAHTVM LEQNYRSTQN ILDAANAVIN
HNNERVPKKL WTENGKGDQI TYYRAQTEHD EANFILSNIQ QLRETKHMAY SDFAVLYRTN
AQSRNIEESL VKANMPYSMV GGHKFYERKE ILDIMAYMSL ITNPDDNAAF ERVVNEPKRG
LGATSLTRLR ELANRLNVSY MKAIGSIELA PSITTKAASK FLTFAEMMHN LRQQSEFLNV
TELTELVMTQ SGYRQMLAEK NDPDSQARLE NLEEFLSVTK EFDDKYQPED PESIDPVTDF
LGTTALMSDL DDFEEGDGAV TLMTLHAAKG LEFPVVFLIG LKEGIFPLSR AMMDEDLLEE
ERRLAYVGIT RAMKKLFLTN AFSRLLYGRT QANEPSRFIA EISPELLETA YSGLSRDKTQ
KKTLPFDRKM QRATATTYQA TPVTKITNGV TGGDQTSWST GDKVSHKKWG VGTVISVSGR
ADDQELKVAF PSEGVKQLLA AFAPIQKVD
