ID   PCRA_STAA8              Reviewed;         730 AA.
AC   Q53727; Q2G1X9;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 3.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=ATP-dependent DNA helicase PcrA;
DE            EC=3.6.4.12;
GN   Name=pcrA; OrderedLocusNames=SAOUHSC_02123;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTANT PCRA3.
RX   PubMed=8232203; DOI=10.1007/bf00280216;
RA   Iordanescu S.;
RT   "Characterization of the Staphylococcus aureus chromosomal gene pcrA,
RT   identified by mutations affecting plasmid pT181 replication.";
RL   Mol. Gen. Genet. 241:185-192(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
CC   -!- FUNCTION: Essential helicase. May act as a helicase in plasmid pT181
CC       replication.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA72091.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M63176; AAA72091.1; ALT_FRAME; Unassigned_DNA.
DR   EMBL; CP000253; ABD31172.1; -; Genomic_DNA.
DR   PIR; S39923; S39923.
DR   RefSeq; WP_000992921.1; NZ_LS483365.1.
DR   RefSeq; YP_500614.1; NC_007795.1.
DR   AlphaFoldDB; Q53727; -.
DR   SMR; Q53727; -.
DR   STRING; 1280.SAXN108_2005; -.
DR   EnsemblBacteria; ABD31172; ABD31172; SAOUHSC_02123.
DR   GeneID; 3921194; -.
DR   KEGG; sao:SAOUHSC_02123; -.
DR   PATRIC; fig|93061.5.peg.1926; -.
DR   eggNOG; COG0210; Bacteria.
DR   HOGENOM; CLU_004585_5_2_9; -.
DR   OMA; YQDTNRT; -.
DR   PRO; PR:Q53727; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0033202; C:DNA helicase complex; IBA:GO_Central.
DR   GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR   GO; GO:0000725; P:recombinational repair; IBA:GO_Central.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01073; pcrA; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA-binding; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..730
FT                   /note="ATP-dependent DNA helicase PcrA"
FT                   /id="PRO_0000102061"
FT   DOMAIN          6..285
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT   DOMAIN          286..560
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00617"
FT   REGION          641..668
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         30..35
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT   BINDING         283
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         61
FT                   /note="T->I: In pcrA3; affects plasmid pT181 replication."
FT   CONFLICT        666..667
FT                   /note="Missing (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   730 AA;  84074 MW;  3C7D6A9501BC3251 CRC64;
     MNALLNHMNT EQSEAVKTTE GPLLIMAGAG SGKTRVLTHR IAYLLDEKDV SPYNVLAITF
     TNKAAREMKE RVQKLVGDQA EVIWMSTFHS MCVRILRRDA DRIGIERNFT IIDPTDQKSV
     IKDVLKNENI DSKKFEPRMF IGAISNLKNE LKTPADAQKE ATDYHSQMVA TVYSGYQRQL
     SRNEALDFDD LIMTTINLFE RVPEVLEYYQ NKFQYIHVDE YQDTNKAQYT LVKLLASKFK
     NLCVVGDSDQ SIYGWRGADI QNILSFEKDY PEANTIFLEQ NYRSTKTILN AANEVIKNNS
     ERKPKGLWTA NTNGEKIHYY EAMTERDEAE FVIREIMKHQ RNGKKYQDMA ILYRTNAQSR
     VLEETFMKSN MPYTMVGGQK FYDRKEIKDL LSYLRIIANS NDDISLQRII NVPKRGVGPS
     SVEKVQNYAL QNNISMFDAL GEADFIGLSK KVTQECLNFY ELIQSLIKEQ EFLEIHEIVD
     EVLQKSGYRE MLERENTLES RSRLENIDEF MSVPKDYEEN TPLEEQSLIN FLTDLSLVAD
     IDEADTENGV TLMTMHSAKG LEFPIVFIMG MEESLFPHIR AIKSEDDHEM QEERRICYVA
     ITRAEEVLYI THATSRMLFG RPQSNMPSRF LKEIPESLLE NHSSGKRQTI QPKAKPFAKR
     GFSQRTTSTK KQVLSSDWNV GDKVMHKAWG EGMVSNVNEK NGSIELDIIF KSQGPKRLLA
     QFAPIEKKED