ID   PCRA_STAEQ              Reviewed;         729 AA.
AC   Q5HN29;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=ATP-dependent DNA helicase PcrA;
DE            EC=3.6.4.12;
GN   Name=pcrA; OrderedLocusNames=SERP1443;
OS   Staphylococcus epidermidis (strain ATCC 35984 / RP62A).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176279;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35984 / RP62A;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- FUNCTION: Essential helicase. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP000029; AAW54831.1; -; Genomic_DNA.
DR   RefSeq; WP_001830418.1; NC_002976.3.
DR   AlphaFoldDB; Q5HN29; -.
DR   SMR; Q5HN29; -.
DR   STRING; 176279.SERP1443; -.
DR   EnsemblBacteria; AAW54831; AAW54831; SERP1443.
DR   GeneID; 50018310; -.
DR   KEGG; ser:SERP1443; -.
DR   eggNOG; COG0210; Bacteria.
DR   HOGENOM; CLU_004585_5_2_9; -.
DR   OMA; YQDTNRT; -.
DR   OrthoDB; 137860at2; -.
DR   Proteomes; UP000000531; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01073; pcrA; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Helicase; Hydrolase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..729
FT                   /note="ATP-dependent DNA helicase PcrA"
FT                   /id="PRO_0000102064"
FT   DOMAIN          6..285
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT   DOMAIN          286..560
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00617"
FT   REGION          642..674
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        644..673
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         30..35
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT   BINDING         283
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   729 AA;  83888 MW;  F91AE893246E1538 CRC64;
     MNALVKNMNS EQSEAVRTTE GPLLIMAGAG SGKTRVLTHR IAYLLDEKDV SPYNILAITF
     TNKAAKEMKA RVEHLVGEEA QVIWMSTFHS MCVRILRRDA DRIGIERNFT IIDPTDQKSV
     IKDVLKSENI DSKRFEPRMF IGAISNLKNE LKTPEDAQKE ANDFHSQMVA TVYKGYQRQL
     SRNEALDFDD LIMTTINLFE RVPETLEYYQ NKFQYIHVDE YQDTNKAQYT LVKLLANKFK
     NLCVVGDSDQ SIYGWRGADI QNILSFEEDY PEAKTIFLEQ NYRSTKNILN AANEVIKHNS
     ERKPKGLWTA NSGGDKIQYY EAMTERDEAE YVVKEIMKHQ RSGKKYSEMA ILYRTNAQSR
     VLEETFMKSN IPYTMVGGQK FYDRKEIKDL LSYLRVIANS NDDISLQRII NVPKRGIGPS
     SVEKIQTYAL QNNISMFDAL AEVDFIGLSK KVTQECISFY EMIQNLIKEQ EFLEISEIVD
     EVLQKSGYRD MLDREQSIES RSRLENLDEF MSVPKDYEEN TPLEEQSLIN FLTDLSLVAD
     IDEADTQNGV TLMTMHSAKG LEFPIVFIMG MEESLFPHIR AIKSEDDHEM EEERRICYVA
     ITRAEELLYI TNATTRMLFG RSQSNMPSRF LKEIPEDLLD SHTGQKRQTI YPKSQPKRGF
     SKRTTSTKKQ VSSSDWKVGD KVMHKAWGEG MVSNVNEKNG SVELDIIFKS EGPKRLLAQF
     APITKKEDS