PCRB_DECAR
ID PCRB_DECAR Reviewed; 333 AA.
AC Q47CW7;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Perchlorate reductase subunit beta;
DE AltName: Full=Perchlorate reductase iron-sulfur subunit;
GN Name=pcrB; OrderedLocusNames=Daro_2583;
OS Dechloromonas aromatica (strain RCB).
OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Azonexaceae;
OC Dechloromonas.
OX NCBI_TaxID=159087;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RCB;
RX PubMed=19650930; DOI=10.1186/1471-2164-10-351;
RA Salinero K.K., Keller K., Feil W.S., Feil H., Trong S., Di Bartolo G.,
RA Lapidus A.;
RT "Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB:
RT indications of a surprisingly complex life-style and cryptic anaerobic
RT pathways for aromatic degradation.";
RL BMC Genomics 10:351-351(2009).
RN [2]
RP IDENTIFICATION, GENE NAME, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, AND
RP BIOTECHNOLOGY.
RX PubMed=16030201; DOI=10.1128/jb.187.15.5090-5096.2005;
RA Bender K.S., Shang C., Chakraborty R., Belchik S.M., Coates J.D.,
RA Achenbach L.A.;
RT "Identification, characterization, and classification of genes encoding
RT perchlorate reductase.";
RL J. Bacteriol. 187:5090-5096(2005).
CC -!- FUNCTION: Component of the perchlorate reductase that catalyzes the
CC reduction of perchlorate to chlorite and allows anaerobic growth on
CC perchlorate as the sole electron acceptor. The beta subunit may be
CC responsible for electron transfer to the catalytic alpha subunit PcrA
CC (Probable). {ECO:0000305|PubMed:16030201}.
CC -!- COFACTOR:
CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137; Evidence={ECO:0000250};
CC Note=Binds 1 [3Fe-4S] cluster. {ECO:0000250};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 3 [4Fe-4S] clusters. {ECO:0000250};
CC -!- SUBUNIT: Heterotrimer of alpha, beta and gamma subunits.
CC {ECO:0000305|PubMed:16030201}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305|PubMed:16030201}.
CC Note=Probably translocated together with PcrA, which possesses a Tat-
CC type signal.
CC -!- BIOTECHNOLOGY: Has potential use in bioremediation of waste sites
CC contaminated with perchlorate, a common component of solid rocket fuel
CC which is a widespread environmental contaminant in water systems in the
CC United States. {ECO:0000269|PubMed:16030201}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000089; AAZ47314.1; -; Genomic_DNA.
DR RefSeq; WP_011288313.1; NC_007298.1.
DR AlphaFoldDB; Q47CW7; -.
DR SMR; Q47CW7; -.
DR STRING; 159087.Daro_2583; -.
DR EnsemblBacteria; AAZ47314; AAZ47314; Daro_2583.
DR KEGG; dar:Daro_2583; -.
DR eggNOG; COG1140; Bacteria.
DR HOGENOM; CLU_043374_5_2_4; -.
DR OMA; NHCTHPS; -.
DR OrthoDB; 1762646at2; -.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009061; P:anaerobic respiration; IEA:InterPro.
DR CDD; cd10555; EBDH_beta; 1.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017839; DMSO_Rdtase_II_Fe-S_su.
DR Pfam; PF13247; Fer4_11; 1.
DR TIGRFAMs; TIGR03478; DMSO_red_II_bet; 1.
DR PROSITE; PS51379; 4FE4S_FER_2; 3.
PE 1: Evidence at protein level;
KW 3Fe-4S; 4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding;
KW Periplasm; Repeat; Transport.
FT CHAIN 1..333
FT /note="Perchlorate reductase subunit beta"
FT /id="PRO_0000422921"
FT DOMAIN 12..40
FT /note="4Fe-4S ferredoxin-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT DOMAIN 128..159
FT /note="4Fe-4S ferredoxin-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT DOMAIN 161..190
FT /note="4Fe-4S ferredoxin-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT REGION 101..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 21
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 24
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 27
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 31
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 137
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 140
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 145
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 149
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000250"
FT BINDING 170
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000250"
FT BINDING 176
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000250"
FT BINDING 180
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 197
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 200
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 212
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 216
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
SQ SEQUENCE 333 AA; 37099 MW; 8BEA9A7ACC8219B9 CRC64;
MANVMKAPKR QLTYVTDLNK CIGCQTCTVA CKKLWTTGPG QDFMYWRNVE TTPGLGYPRN
WQTKGGGYKN GELQKGKIPP MIDYGIPFEF DYAGRLFEGK KERVRPSPTP RSAPNWDEDQ
GAGEYPNNSF FYLPRMCNHC TKPACLEACP NEAIYKREQD GIVVIHQDKC KGAQACVQSC
PYAKPYFNPV ANKANKCIGC FPRIEQGVAP GCVAQCVGRA MHVGFIDDTN SSVHKLIRLY
KVALPLHPEF GTEPNVFYVP PVLGPRMELP NGELSTDPKI PLAQLEGLFG KQVRDVLAIL
QTEREKKMKG LASDLMDVLI GRRSADMMIS PLT
