PCS1_SCHPO
ID PCS1_SCHPO Reviewed; 261 AA.
AC O13684; Q870L5; Q9UTP7;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 4.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Monopolin complex subunit pcs1;
DE AltName: Full=Chromosome segregation protein 1;
GN Name=pcs1; ORFNames=SPAC11E3.03;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12689592; DOI=10.1016/s1534-5807(03)00086-8;
RA Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B.,
RA Schleiffer A., Tanaka T.U., Nasmyth K.;
RT "Kinetochore recruitment of two nucleolar proteins is required for homolog
RT segregation in meiosis I.";
RL Dev. Cell 4:535-548(2003).
RN [4]
RP FUNCTION, INTERACTION WITH MDE4, AND SUBCELLULAR LOCATION.
RX PubMed=17627824; DOI=10.1016/j.cub.2007.06.044;
RA Gregan J., Riedel C.G., Pidoux A.L., Katou Y., Rumpf C., Schleiffer A.,
RA Kearsey S.E., Shirahige K., Allshire R.C., Nasmyth K.;
RT "The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to
RT prevent merotelic orientation.";
RL Curr. Biol. 17:1190-1200(2007).
CC -!- FUNCTION: The monopolin-like pcs1/mde4 complex is essential for
CC accurate chromosome segregation during mitosis and meiosis II. May
CC clamp together microtubule binding sites on the same kinetochore,
CC preventing merotelic attachment of microtubules. In contrast to its
CC S.cerevisiae ortholog CSM1, is not required ofr mono-orientation during
CC meiosis I. {ECO:0000269|PubMed:12689592, ECO:0000269|PubMed:17627824}.
CC -!- SUBUNIT: Component of a monopolin-like complex composed of pcs1 and
CC mde4. The complex associates with the kinetochore.
CC -!- INTERACTION:
CC O13684; O43068: mde4; NbExp=3; IntAct=EBI-2211100, EBI-2211118;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12689592}.
CC Chromosome, centromere {ECO:0000269|PubMed:12689592}. Note=Nucleolar
CC during G2 (PubMed:12689592). Localizes to the centromeres throughout
CC most stages of vegetative growth and meiotic development apart from the
CC horse tail stage of prophase I (PubMed:12689592). Localizes to the
CC central core of the centromere (PubMed:17627824).
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CU329670; CAD88639.2; -; Genomic_DNA.
DR PIR; T37530; T37530.
DR PIR; T37531; T37531.
DR RefSeq; NP_001018298.2; NM_001020359.2.
DR AlphaFoldDB; O13684; -.
DR SMR; O13684; -.
DR BioGRID; 280638; 10.
DR DIP; DIP-47341N; -.
DR IntAct; O13684; 7.
DR STRING; 4896.SPAC11E3.03.1; -.
DR MaxQB; O13684; -.
DR PaxDb; O13684; -.
DR EnsemblFungi; SPAC11E3.03.1; SPAC11E3.03.1:pep; SPAC11E3.03.
DR GeneID; 3361562; -.
DR KEGG; spo:SPAC11E3.03; -.
DR PomBase; SPAC11E3.03; -.
DR VEuPathDB; FungiDB:SPAC11E3.03; -.
DR eggNOG; ENOG502R6WM; Eukaryota.
DR HOGENOM; CLU_093249_0_0_1; -.
DR InParanoid; O13684; -.
DR OMA; SARIMKA; -.
DR PRO; PR:O13684; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0034506; C:chromosome, centromeric core domain; IDA:PomBase.
DR GO; GO:0000775; C:chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0072686; C:mitotic spindle; IDA:PomBase.
DR GO; GO:0033551; C:monopolin complex; IDA:PomBase.
DR GO; GO:0005730; C:nucleolus; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:1990644; F:microtubule site clamp; IPI:PomBase.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IMP:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0045143; P:homologous chromosome segregation; IBA:GO_Central.
DR GO; GO:0045144; P:meiotic sister chromatid segregation; IMP:PomBase.
DR GO; GO:1990893; P:mitotic chromosome centromere condensation; IMP:PomBase.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:PomBase.
DR Gene3D; 3.90.1150.80; -; 1.
DR InterPro; IPR040349; Csm1/Pcs1.
DR InterPro; IPR020981; Csm1/Pcs1_C.
DR InterPro; IPR038608; Csm1/Pcs1_C_sf.
DR PANTHER; PTHR28006; PTHR28006; 1.
DR Pfam; PF12539; Csm1; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Meiosis;
KW Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..261
FT /note="Monopolin complex subunit pcs1"
FT /id="PRO_0000058266"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 91..170
FT /evidence="ECO:0000255"
FT COMPBIAS 1..23
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 261 AA; 30418 MW; 788A079D2920CE35 CRC64;
MRKNNMQTSK DSELKEQAKG KSSKLIHKLP KQRTRISQGQ MHSTQDFVNN EDQDAYSVRE
NENELHINNS GMSELNKKLQ LPNVELSTLS HTQEQEFNEL NKLIRKINEL QEFYLLEDLA
KPVTNAGADA DDTIVKDLKK ELENEKKANH SLKNELLKTR EQIKNYSKIN ILIKELFGLE
VADCIEDEDG YRFNCKNTGR RGTLEYQLLL DDQNFTFTPR LNVQTDEELM KHLPDYLLEE
IIFTKEQGKL FSARLMKALQ D
