ID   PCTM2_ECOLX             Reviewed;         217 AA.
AC   P0DV27;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=Pycsar effector protein Ec303145PycTM;
DE            Short=Ec303145PycTM {ECO:0000303|PubMed:34644530};
GN   Name=pycTM {ECO:0000303|PubMed:34644530}; ORFNames=Ga0100619_101299;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=303145;
RA   Hazen T.H., Donnenberg M., Nataro J., Kaper J., Rasko D.;
RT   "Emergence of novel diverse phylogenomic lineages of EPEC.";
RL   Submitted (FEB-2014) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, ANTIVIRAL DEFENSE, AND CLASSIFICATION.
RC   STRAIN=303145;
RX   PubMed=34644530; DOI=10.1016/j.cell.2021.09.031;
RA   Tal N., Morehouse B.R., Millman A., Stokar-Avihail A., Avraham C.,
RA   Fedorenko T., Yirmiya E., Herbst E., Brandis A., Mehlman T.,
RA   Oppenheimer-Shaanan Y., Keszei A.F.A., Shao S., Amitai G., Kranzusch P.J.,
RA   Sorek R.;
RT   "Cyclic CMP and cyclic UMP mediate bacterial immunity against phages.";
RL   Cell 0:0-0(2021).
CC   -!- FUNCTION: Pycsar (pyrimidine cyclase system for antiphage resistance)
CC       provides immunity against bacteriophage. The pyrimidine cyclase (PycC)
CC       synthesizes cyclic nucleotides in response to infection; these serve as
CC       specific second messenger signals. The signals activate the adjacent
CC       effector, leading to bacterial cell death and abortive phage infection.
CC       A clade E Pycsar system. {ECO:0000269|PubMed:34644530}.
CC   -!- FUNCTION: The effector component of a two-gene Pycsar system.
CC       Expression of this and adjacent cytidylate cyclase Ec303145PycC (AC
CC       P0DV26) confers resistance to bacteriophage P1, T5, lambda-vir and
CC       phi27 (PubMed:34644530). Probably only responds to cCMP (produced by
CC       its cognate NTP cyclase), acts by impairing membrane integrity
CC       (Probable). {ECO:0000269|PubMed:34644530, ECO:0000305|PubMed:34644530}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000255}.
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DR   EMBL; JHSG01000012; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Transmembrane; Transmembrane helix.
FT   CHAIN           1..217
FT                   /note="Pycsar effector protein Ec303145PycTM"
FT                   /id="PRO_0000455231"
FT   TRANSMEM        29..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        187..207
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   217 AA;  24564 MW;  EE552913CBA15D6F CRC64;
     MDKDRVELLI KSVIRTDGYL NYANTKSTIL LTLASGVLAT ASLNLSKLLP LSLDKLSTSS
     FVSFSFFLVI GVLINILSVV RTLKAISPYL KNSDKENIFS FVDIVHYNSS SDTYSEKIKK
     LDYSHLGEQL TSLNYNLSVG LLDKYKNQRR AIILLKISMA SFFLSIVVPW FFYCFDISLT
     EKGPIVIMLL FIISLVVFIV FLLTLIIYLL YLGKDKK