ID   PCTM_GULMO              Reviewed;         196 AA.
AC   P0DV43;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=Pycsar effector protein GmPycTM;
DE            Short=GmPycTM {ECO:0000303|PubMed:34644530};
GN   Name=pycTM {ECO:0000303|PubMed:34644530};
GN   ORFNames=Ga0100804_10531 {ECO:0000303|PubMed:26586874};
OS   Gulbenkiania mobilis.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales;
OC   Chromobacteriaceae; Gulbenkiania.
OX   NCBI_TaxID=397457;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MB1;
RX   PubMed=26586874; DOI=10.1128/genomea.01295-15;
RA   Saxena R., Chaudhary N., Dhakan D.B., Sharma V.K.;
RT   "Draft Genome Sequence of Gulbenkiania mobilis Strain MB1, a Sulfur-
RT   Metabolizing Thermophile Isolated from a Hot Spring in Central India.";
RL   Genome Announc. 3:0-0(2015).
RN   [2]
RP   PROBABLE FUNCTION, AND CLASSIFICATION.
RC   STRAIN=MB1;
RX   PubMed=34644530; DOI=10.1016/j.cell.2021.09.031;
RA   Tal N., Morehouse B.R., Millman A., Stokar-Avihail A., Avraham C.,
RA   Fedorenko T., Yirmiya E., Herbst E., Brandis A., Mehlman T.,
RA   Oppenheimer-Shaanan Y., Keszei A.F.A., Shao S., Amitai G., Kranzusch P.J.,
RA   Sorek R.;
RT   "Cyclic CMP and cyclic UMP mediate bacterial immunity against phages.";
RL   Cell 0:0-0(2021).
CC   -!- FUNCTION: Pycsar (pyrimidine cyclase system for antiphage resistance)
CC       provides immunity against bacteriophage. The pyrimidine cyclase (PycC)
CC       synthesizes cyclic nucleotides in response to infection; these serve as
CC       specific second messenger signals. The signals activate the adjacent
CC       effector, leading to bacterial cell death and abortive phage infection.
CC       A clade C Pycsar system. {ECO:0000305|PubMed:34644530}.
CC   -!- FUNCTION: The effector gene of a two-gene Pycsar system. Expression of
CC       this and adjacent uridylate cyclase GmPycC (AC P0DV42) probably confers
CC       resistance to bacteriophage. The genes are probably only expressed in
CC       response to bacteriophage infection. Probably only responds to cUMP
CC       (produced by its cognate NTP cyclase), acts by impairing membrane
CC       integrity. {ECO:0000305|PubMed:34644530}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; LIVN01000053; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR043760; DUF5706.
DR   Pfam; PF18967; DUF5706; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Transmembrane; Transmembrane helix.
FT   CHAIN           1..196
FT                   /note="Pycsar effector protein GmPycTM"
FT                   /id="PRO_0000455234"
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        82..102
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   196 AA;  22222 MW;  CD79CE79C7DD59E2 CRC64;
     MSEENKLSES EIKEEAVQTL ERINFWISNC DTKISFSLAF AGILLGGFFS SGIITGSLNK
     LMKGLKEIDK DTPYLKIQYL EITTIVLVVF IILMIVSLTY LFRGKKGSID TGVFNEADLS
     KDSILFFGTI QNKSFISFKN SVIEIKKDDL VNDYLSQVYI NSKICNRKFT LYNKGVNWLI
     ASTIVFIILN GMFLFL