ID   PCTM_MICEH              Reviewed;          59 AA.
AC   A0A1C5G2D0;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2016, sequence version 1.
DT   03-AUG-2022, entry version 11.
DE   RecName: Full=Pycsar effector protein MePycTM;
DE            Short=MePycTM {ECO:0000303|PubMed:34644530};
GN   Name=pycTM {ECO:0000303|PubMed:34644530};
GN   ORFNames=GA0070610_0181 {ECO:0000303|Ref.1};
OS   Micromonospora echinofusca.
OC   Bacteria; Actinobacteria; Micromonosporales; Micromonosporaceae;
OC   Micromonospora.
OX   NCBI_TaxID=47858;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 43913 / CIP 108946 / JCM 3327 / NBRC 14267 / 71-m68;
RA   Varghese N.;
RL   Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROBABLE FUNCTION, AND CLASSIFICATION.
RC   STRAIN=DSM 43913 / CIP 108946 / JCM 3327 / NBRC 14267 / 71-m68;
RX   PubMed=34644530; DOI=10.1016/j.cell.2021.09.031;
RA   Tal N., Morehouse B.R., Millman A., Stokar-Avihail A., Avraham C.,
RA   Fedorenko T., Yirmiya E., Herbst E., Brandis A., Mehlman T.,
RA   Oppenheimer-Shaanan Y., Keszei A.F.A., Shao S., Amitai G., Kranzusch P.J.,
RA   Sorek R.;
RT   "Cyclic CMP and cyclic UMP mediate bacterial immunity against phages.";
RL   Cell 0:0-0(2021).
CC   -!- FUNCTION: Pycsar (pyrimidine cyclase system for antiphage resistance)
CC       provides immunity against bacteriophage. The pyrimidine cyclase (PycC)
CC       synthesizes cyclic nucleotides in response to infection; these serve as
CC       specific second messenger signals. The signals activate the adjacent
CC       effector, leading to bacterial cell death and abortive phage infection.
CC       A clade D Pycsar system. {ECO:0000305|PubMed:34644530}.
CC   -!- FUNCTION: The effector gene of a two-gene Pycsar system. Expression of
CC       this and adjacent uridylate cyclase MePycC (AC A0A1C5G2V9) probably
CC       confers resistance to bacteriophage. The genes are probably only
CC       expressed in response to bacteriophage infection. Probably only
CC       responds to cUMP (produced by its cognate NTP cyclase), acts by
CC       impairing membrane integrity. {ECO:0000305|PubMed:34644530}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000255}.
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DR   EMBL; LT607733; SCG13989.1; -; Genomic_DNA.
DR   EnsemblBacteria; SCG13989; SCG13989; GA0070610_0181.
DR   Proteomes; UP000198251; Chromosome i.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR043760; DUF5706.
DR   Pfam; PF18967; DUF5706; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Cell membrane; Membrane; Nucleotide-binding;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..59
FT                   /note="Pycsar effector protein MePycTM"
FT                   /id="PRO_0000455235"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   59 AA;  6558 MW;  790D8C96448CEBDD CRC64;
     MDTFTALTAD EERFERHLAE QVWANSKVAR AKFRRVAVAI YLLGAAMLSS GAAVLAYRL