PCTM_PROTD
ID PCTM_PROTD Reviewed; 202 AA.
AC A0A4Q9KQH5;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 31-JUL-2019, sequence version 1.
DT 03-AUG-2022, entry version 8.
DE RecName: Full=Pycsar effector protein PtPycTM;
DE Short=PtPycTM {ECO:0000303|PubMed:34644530};
GN Name=pycTM {ECO:0000303|PubMed:34644530};
GN ORFNames=ET996_01255 {ECO:0000303|Ref.1};
OS Propioniciclava tarda.
OC Bacteria; Actinobacteria; Propionibacteriales; Propionibacteriaceae;
OC Propioniciclava.
OX NCBI_TaxID=433330;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22130 / JCM 15804 / WR061;
RA Huptas C., Wenning M., Breitenwieser F., Doll E., Von Neubeck M.,
RA Busse H.-J., Scherer S.;
RT "Lactibacter flavus gen. nov., sp. nov., a novel bacterium of the family
RT Propionibacteriaceae isolated from raw milk and dairy products.";
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PROBABLE FUNCTION, AND CLASSIFICATION.
RC STRAIN=DSM 22130 / JCM 15804 / WR061;
RX PubMed=34644530; DOI=10.1016/j.cell.2021.09.031;
RA Tal N., Morehouse B.R., Millman A., Stokar-Avihail A., Avraham C.,
RA Fedorenko T., Yirmiya E., Herbst E., Brandis A., Mehlman T.,
RA Oppenheimer-Shaanan Y., Keszei A.F.A., Shao S., Amitai G., Kranzusch P.J.,
RA Sorek R.;
RT "Cyclic CMP and cyclic UMP mediate bacterial immunity against phages.";
RL Cell 0:0-0(2021).
CC -!- FUNCTION: Pycsar (pyrimidine cyclase system for antiphage resistance)
CC provides immunity against bacteriophage. The pyrimidine cyclase (PycC)
CC synthesizes cyclic nucleotides in response to infection; these serve as
CC specific second messenger signals. The signals activate the adjacent
CC effector, leading to bacterial cell death and abortive phage infection.
CC A clade D Pycsar system. {ECO:0000305|PubMed:34644530}.
CC -!- FUNCTION: The effector gene of a two-gene Pycsar system. Expression of
CC this and adjacent uridylate cyclase PtPycC (AC A0A4V2JTK3) probably
CC confers resistance to bacteriophage. The genes are probably only
CC expressed in response to bacteriophage infection. Probably only
CC responds to cUMP (produced by its cognate NTP cyclase), acts by
CC impairing membrane integrity. {ECO:0000305|PubMed:34644530}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
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DR EMBL; SDMR01000001; TBT96320.1; -; Genomic_DNA.
DR Proteomes; UP000291933; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR InterPro; IPR043760; DUF5706.
DR Pfam; PF18967; DUF5706; 1.
PE 3: Inferred from homology;
KW Antiviral defense; Cell membrane; Membrane; Nucleotide-binding;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..202
FT /note="Pycsar effector protein PtPycTM"
FT /id="PRO_0000455236"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 202 AA; 21119 MW; 8B176982A6C43060 CRC64;
MSTLLGWLGR LWFGSLGTAR PDPSTAVAAA GSTDVTSTQD AWAVLSLVNE WVKHSEAKLG
VVLAFVGVMA AGLITIAADI PCPSLVILCI EGAAAVLILA SAVLASLGLL PRFKGQSEEA
QMNPLFYGDV ANHFKGKSEE YVTALSGVIG SQDALIRQIA RQVHANADVA SRKYLWANRS
ILVGMLGLVC LFVLAAGVAL GW
