ID   PCTM_RHIS2              Reviewed;         175 AA.
AC   A0A4R2UGS4;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 2.
DT   03-AUG-2022, entry version 5.
DE   RecName: Full=Pycsar effector protein RsPycTM;
DE            Short=RsPycTM {ECO:0000303|PubMed:34644530};
GN   Name=pycTM {ECO:0000303|PubMed:34644530};
GN   ORFNames=C8J34_10223 {ECO:0000303|Ref.1};
OS   Rhizobium sp. (strain PP-F2F-G36).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=2135649;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PP-F2F-G36;
RA   Whitman W.;
RT   "Genomic Encyclopedia of Type Strains, Phase IV (KMG-V): Genome sequencing
RT   to study the core and pangenomes of soil and plant-associated
RT   prokaryotes.";
RL   Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND CLASSIFICATION.
RC   STRAIN=PP-F2F-G36;
RX   PubMed=34644530; DOI=10.1016/j.cell.2021.09.031;
RA   Tal N., Morehouse B.R., Millman A., Stokar-Avihail A., Avraham C.,
RA   Fedorenko T., Yirmiya E., Herbst E., Brandis A., Mehlman T.,
RA   Oppenheimer-Shaanan Y., Keszei A.F.A., Shao S., Amitai G., Kranzusch P.J.,
RA   Sorek R.;
RT   "Cyclic CMP and cyclic UMP mediate bacterial immunity against phages.";
RL   Cell 0:0-0(2021).
CC   -!- FUNCTION: Pycsar (pyrimidine cyclase system for antiphage resistance)
CC       provides immunity against bacteriophage. The pyrimidine cyclase (PycC)
CC       synthesizes cyclic nucleotides in response to infection; these serve as
CC       specific second messenger signals. The signals activate the nearby
CC       effector, leading to bacterial cell death and abortive phage infection.
CC       A clade A Pycsar system. {ECO:0000305|PubMed:34644530}.
CC   -!- FUNCTION: The effector gene of a two-gene Pycsar system. Expression of
CC       this and uridylate cyclase RsPycC (AC A0A4R2TZQ0) probably confers
CC       resistance to bacteriophage. The genes are probably only expressed in
CC       response to bacteriophage infection. Probably only responds to cUMP
CC       (produced by its cognate NTP cyclase), acts by impairing membrane
CC       integrity. {ECO:0000305|PubMed:34644530}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000255}.
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DR   EMBL; SLYE01000002; TCQ09629.1; -; Genomic_DNA.
DR   Proteomes; UP000294769; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR043760; DUF5706.
DR   Pfam; PF18967; DUF5706; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Transmembrane; Transmembrane helix.
FT   CHAIN           1..175
FT                   /note="Pycsar effector protein RsPycTM"
FT                   /id="PRO_0000455238"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   175 AA;  19558 MW;  0FF4804000BBEF7C CRC64;
     MSANHTRMID LSKFAEAKNA ALLTFCSVWM GAIITLLRSP DELPLGFDYA FKASLTVLFI
     AAIISLKSLM PKFLNQVHKR EDEYKNLLYF GDIDQIGTGA YPGMAADLYT PTEGASATPT
     YLHDLAVQTA IHAKIAHRKF RLFNWAGSLV LFAFGIMMVP PILFCIRWAV NRLHC