ID   PCTM_TREPO              Reviewed;         115 AA.
AC   A0A1T4LJG1;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2017, sequence version 1.
DT   03-AUG-2022, entry version 9.
DE   RecName: Full=Pycsar effector protein TpPycTM;
DE            Short=TpPycTM {ECO:0000303|PubMed:34644530};
GN   Name=pycTM {ECO:0000303|PubMed:34644530};
GN   ORFNames=SAMN02745149_01625 {ECO:0000303|Ref.1};
OS   Treponema porcinum.
OC   Bacteria; Spirochaetes; Spirochaetales; Treponemataceae; Treponema.
OX   NCBI_TaxID=261392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-908 / CIP 108245 / JCM 2342 / 14V28;
RA   Peterson S.W.;
RL   Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND CLASSIFICATION.
RC   STRAIN=ATCC BAA-908 / CIP 108245 / JCM 2342 / 14V28;
RX   PubMed=34644530; DOI=10.1016/j.cell.2021.09.031;
RA   Tal N., Morehouse B.R., Millman A., Stokar-Avihail A., Avraham C.,
RA   Fedorenko T., Yirmiya E., Herbst E., Brandis A., Mehlman T.,
RA   Oppenheimer-Shaanan Y., Keszei A.F.A., Shao S., Amitai G., Kranzusch P.J.,
RA   Sorek R.;
RT   "Cyclic CMP and cyclic UMP mediate bacterial immunity against phages.";
RL   Cell 0:0-0(2021).
CC   -!- FUNCTION: Pycsar (pyrimidine cyclase system for antiphage resistance)
CC       provides immunity against bacteriophage. The pyrimidine cyclase (PycC)
CC       synthesizes cyclic nucleotides in response to infection; these serve as
CC       specific second messenger signals. The signals activate the adjacent
CC       effector, leading to bacterial cell death and abortive phage infection.
CC       A clade C Pycsar system. {ECO:0000305|PubMed:34644530}.
CC   -!- FUNCTION: The effector gene of a two-gene Pycsar system. Expression of
CC       this and adjacent uridylate cyclase TpPycC (AC A0A1T4LJ54) probably
CC       confers resistance to bacteriophage. The genes are probably only
CC       expressed in response to bacteriophage infection. Probably only
CC       responds to cUMP (produced by its cognate NTP cyclase), acts by
CC       impairing membrane integrity. {ECO:0000305|PubMed:34644530}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000255}.
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DR   EMBL; FUWG01000011; SJZ54756.1; -; Genomic_DNA.
DR   EnsemblBacteria; SJZ54756; SJZ54756; SAMN02745149_01625.
DR   Proteomes; UP000190423; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Antiviral defense; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..115
FT                   /note="Pycsar effector protein TpPycTM"
FT                   /id="PRO_0000455240"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        74..94
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   115 AA;  12981 MW;  BFC46DA3802381D2 CRC64;
     MIGLTKVCWT MSKKEVVDNS IKIEYLRSSF DNVQSLIQFA DQKIGNLLLI DTITVGIFIT
     YATNYELSLK DLTVWNILLF ITGLIFTVSS VILVYKSIIK VVCSAQSELG LFYKS