ID   PCXA_BURCE              Reviewed;         197 AA.
AC   P15109;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Protocatechuate 3,4-dioxygenase alpha chain;
DE            EC=1.13.11.3;
DE   AltName: Full=3,4-PCD;
GN   Name=pcaG;
OS   Burkholderia cepacia (Pseudomonas cepacia).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2808303; DOI=10.1128/jb.171.11.5915-5921.1989;
RA   Zylstra G.J., Olsen R.H., Ballou D.P.;
RT   "Genetic organization and sequence of the Pseudomonas cepacia genes for the
RT   alpha and beta subunits of protocatechuate 3,4-dioxygenase.";
RL   J. Bacteriol. 171:5915-5921(1989).
CC   -!- FUNCTION: Plays an essential role in the utilization of numerous
CC       aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate + 2
CC         H(+); Xref=Rhea:RHEA:10084, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:36241, ChEBI:CHEBI:57496; EC=1.13.11.3;
CC   -!- COFACTOR:
CC       Name=Fe(3+); Xref=ChEBI:CHEBI:29034;
CC       Note=Binds Fe(3+) ion per subunit.;
CC   -!- PATHWAY: Aromatic compound metabolism; beta-ketoadipate pathway; 3-
CC       carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate: step 1/1.
CC   -!- SUBUNIT: The enzyme is an oligomer of 12 copies of the alpha and beta
CC       chains.
CC   -!- SIMILARITY: Belongs to the intradiol ring-cleavage dioxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; M30791; AAA25925.1; -; Genomic_DNA.
DR   PIR; A33487; A33487.
DR   AlphaFoldDB; P15109; -.
DR   SMR; P15109; -.
DR   STRING; 292.DM42_3793; -.
DR   eggNOG; COG3485; Bacteria.
DR   UniPathway; UPA00157; UER00264.
DR   GO; GO:0008199; F:ferric iron binding; IEA:InterPro.
DR   GO; GO:0018578; F:protocatechuate 3,4-dioxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042952; P:beta-ketoadipate pathway; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.60.130.10; -; 1.
DR   InterPro; IPR000627; Intradiol_dOase_C.
DR   InterPro; IPR015889; Intradiol_dOase_core.
DR   InterPro; IPR012786; Protocat_dOase_a.
DR   SUPFAM; SSF49482; SSF49482; 1.
DR   TIGRFAMs; TIGR02423; protocat_alph; 1.
DR   PROSITE; PS00083; INTRADIOL_DIOXYGENAS; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Iron; Oxidoreductase.
FT   CHAIN           1..197
FT                   /note="Protocatechuate 3,4-dioxygenase alpha chain"
FT                   /id="PRO_0000085096"
FT   BINDING         130
FT                   /ligand="3,4-dihydroxybenzoate"
FT                   /ligand_id="ChEBI:CHEBI:36241"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   197 AA;  21394 MW;  A00C1BCD420ECFC0 CRC64;
     MTTLKQTPSQ TVGPYFAYGL CPQQYGYDLK SLFTPTIAAP HADGEHVLLV GQVFDGDGNV
     VSDAMLEFTQ VDGAGRFPAS RDDVAKSGFT GFARVGTGTD AQHRFVVETV KPGRIAADEA
     PHINVTVMMR GILTHAFTRV YFDDEAAANA ADPVLNLVPA ERRATLVAKR DAQPGRPVVY
     RFDVRMQGPD ETVFFDV