ID   PCXA_SYNY3              Reviewed;         440 AA.
AC   P75028;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 124.
DE   RecName: Full=Proton extrusion protein PcxA;
GN   Name=pcxA; Synonyms=cotA; OrderedLocusNames=slr1596;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8633006; DOI=10.1073/pnas.93.9.4006;
RA   Katoh A., Lee K.S., Fukuzawa H., Ohyama K., Ogawa T.;
RT   "cemA homologue essential to CO2 transport in the cyanobacterium
RT   Synechocystis PCC6803.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:4006-4010(1996).
RN   [2]
RP   SEQUENCE REVISION TO N-TERMINUS, AND SUBCELLULAR LOCATION.
RX   PubMed=9190798; DOI=10.1128/jb.179.12.3845-3850.1997;
RA   Sonoda M., Kitano K., Katoh A., Katoh H., Ohkawa H., Ogawa T.;
RT   "Size of cotA and identification of the gene product in Synechocystis sp.
RT   strain PCC6803.";
RL   J. Bacteriol. 179:3845-3850(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=8808935; DOI=10.1128/jb.178.18.5452-5455.1996;
RA   Katoh A., Sonoda M., Katoh H., Ogawa T.;
RT   "Absence of light-induced proton extrusion in a cotA-less mutant of
RT   Synechocystis sp. strain PCC6803.";
RL   J. Bacteriol. 178:5452-5455(1996).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=9683474; DOI=10.1128/jb.180.15.3799-3803.1998;
RA   Sonoda M., Katoh H., Vermaas W., Schmetterer G., Ogawa T.;
RT   "Photosynthetic electron transport involved in PxcA-dependent proton
RT   extrusion in Synechocystis sp. Strain PCC6803: effect of pxcA inactivation
RT   on CO2, HCO3-, and NO3- uptake.";
RL   J. Bacteriol. 180:3799-3803(1998).
CC   -!- FUNCTION: Involved in light-induced Na(+)-dependent proton extrusion.
CC       Also seems to be involved in CO(2) transport.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:9190798};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:9190798}.
CC   -!- SIMILARITY: Belongs to the Cema family. {ECO:0000305}.
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DR   EMBL; D63838; BAA09896.1; -; Genomic_DNA.
DR   EMBL; BA000022; BAA16993.1; -; Genomic_DNA.
DR   PIR; S74953; S74953.
DR   AlphaFoldDB; P75028; -.
DR   IntAct; P75028; 16.
DR   STRING; 1148.1652068; -.
DR   TCDB; 9.B.73.1.2; the chloroplast envelope/cyanobacterial membrane protein (cema) family.
DR   PaxDb; P75028; -.
DR   EnsemblBacteria; BAA16993; BAA16993; BAA16993.
DR   KEGG; syn:slr1596; -.
DR   eggNOG; ENOG502Z8DN; Bacteria.
DR   InParanoid; P75028; -.
DR   OMA; PANRDFI; -.
DR   PhylomeDB; P75028; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015078; F:proton transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01308; CemA; 1.
DR   InterPro; IPR004282; CemA.
DR   PANTHER; PTHR33650; PTHR33650; 1.
DR   Pfam; PF03040; CemA; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Hydrogen ion transport; Ion transport;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..440
FT                   /note="Proton extrusion protein PcxA"
FT                   /id="PRO_0000216670"
FT   TRANSMEM        217..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        317..339
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        352..374
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        402..421
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   440 AA;  51117 MW;  67105B2ABEB13452 CRC64;
     MDLTNWWQGA TQWFGRSSQK SLEQAFRSAL KIKEIEDQYF QGKKIGPENC DYSADTVTYF
     ANQIQRHLRK IEQEIYHLNS DQEFVKILSL DPAVKQDPQT EYVLNQLQFI DDILQRYDGE
     LPQVSPPKQI ANGGVLDLPA ITANKQRQIN KKRRDGFQYI RREDTQQKVD TATQKSGVLP
     RSFLRTIDRL KREMDPQSSD TEQKVLKQYR NSRYKTALSI KFVLTLIIVP LLAHQLTKTF
     FLLPSVESFF ERNSEVVFIN QSMETEAYEE LSHFEESLRF RELLGFGEKL SPEAKEEKLA
     EKAKEISESY RRVSTNAIAN IFADIFSLVA FSLVLVNSQR EIEVLKEFID EIVYGLSDSA
     KAFLIILFTD MFVGFHSPHG WEVILASIAR HFGLPENQDF NFLFIATFPV ILDTVFKYWI
     FRYLNSISPS AVATYRNMNE