PCYAA_EUGGR
ID PCYAA_EUGGR Reviewed; 1019 AA.
AC Q8S9F2; Q2P9N1;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Photoactivated adenylate cyclase subunit alpha;
DE EC=4.6.1.1;
DE AltName: Full=Photoactivated adenylyl cyclase subunit alpha;
GN Name=pacA {ECO:0000312|EMBL:CAJ57393.1};
OS Euglena gracilis.
OC Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC Euglenales; Euglenaceae; Euglena.
OX NCBI_TaxID=3039;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAB85619.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-31, FUNCTION, CATALYTIC
RP ACTIVITY, COFACTOR, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES,
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=Z / UTEX 753 {ECO:0000312|EMBL:BAB85619.1};
RX PubMed=11875575; DOI=10.1038/4151047a;
RA Iseki M., Matsunaga S., Murakami A., Ohno K., Shiga K., Yoshida K.,
RA Sugai M., Takahashi T., Hori T., Watanabe M.;
RT "A blue-light-activated adenylyl cyclase mediates photoavoidance in Euglena
RT gracilis.";
RL Nature 415:1047-1051(2002).
RN [2] {ECO:0000305, ECO:0000312|EMBL:CAJ57393.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RC STRAIN=SAG 1224-5/9F;
RX PubMed=16121285; DOI=10.1039/b502002f;
RA Ntefidou M., Haeder D.-P.;
RT "Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena
RT gracilis mutant strains.";
RL Photochem. Photobiol. Sci. 4:732-739(2005).
CC -!- FUNCTION: Acts as a blue light photoreceptor for the step-up
CC photophobic response. Mediates photoavoidance.
CC {ECO:0000269|PubMed:11875575}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP = 3',5'-cyclic AMP + diphosphate; Xref=Rhea:RHEA:15389,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58165; EC=4.6.1.1;
CC Evidence={ECO:0000269|PubMed:11875575};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000269|PubMed:11875575};
CC -!- ACTIVITY REGULATION: Activity increased by up to 80-fold under blue
CC light. {ECO:0000269|PubMed:11875575}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.5 uM for ATP {ECO:0000269|PubMed:11875575};
CC Vmax=3.5 nmol/min/mg enzyme {ECO:0000269|PubMed:11875575};
CC -!- SUBUNIT: Heterotetramer of two alpha and two beta subunits.
CC {ECO:0000269|PubMed:11875575}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC {ECO:0000269|PubMed:11875575, ECO:0000269|PubMed:16121285}.
CC Note=Paraflagellar body, flagellum and paraxonemal bodies (PABs).
CC -!- MISCELLANEOUS: The 1224-5/9F strain is deficient in phototaxis. It is
CC not known if this is due to defective adenylate cyclase activity or
CC defective BLUF domains in the beta subunit of this protein.
CC {ECO:0000269|PubMed:16121285}.
CC -!- SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase
CC family. {ECO:0000255|PROSITE-ProRule:PRU00099}.
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DR EMBL; AB031225; BAB85619.1; -; mRNA.
DR EMBL; AM181334; CAJ57393.1; -; mRNA.
DR AlphaFoldDB; Q8S9F2; -.
DR SMR; Q8S9F2; -.
DR BRENDA; 4.6.1.1; 2197.
DR SABIO-RK; Q8S9F2; -.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0004016; F:adenylate cyclase activity; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0009882; F:blue light photoreceptor activity; IEA:InterPro.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0009881; F:photoreceptor activity; IDA:UniProtKB.
DR GO; GO:0006171; P:cAMP biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR CDD; cd07302; CHD; 2.
DR Gene3D; 3.30.70.1230; -; 2.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001054; A/G_cyclase.
DR InterPro; IPR036046; Acylphosphatase-like_dom_sf.
DR InterPro; IPR007024; BLUF_domain.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR029787; Nucleotide_cyclase.
DR Pfam; PF04940; BLUF; 2.
DR Pfam; PF00211; Guanylate_cyc; 2.
DR SMART; SM01034; BLUF; 2.
DR SUPFAM; SSF54975; SSF54975; 2.
DR SUPFAM; SSF55073; SSF55073; 2.
DR PROSITE; PS50925; BLUF; 2.
DR PROSITE; PS50125; GUANYLATE_CYCLASE_2; 2.
PE 1: Evidence at protein level;
KW ATP-binding; cAMP biosynthesis; Cell projection; Chromophore; Cilium;
KW Direct protein sequencing; FAD; Flagellum; Flavoprotein; Lyase;
KW Nucleotide-binding; Photoreceptor protein; Receptor; Repeat;
KW Sensory transduction.
FT CHAIN 1..1019
FT /note="Photoactivated adenylate cyclase subunit alpha"
FT /id="PRO_0000195717"
FT DOMAIN 55..148
FT /note="BLUF 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT DOMAIN 204..332
FT /note="Guanylate cyclase 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT DOMAIN 467..559
FT /note="BLUF 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT DOMAIN 615..744
FT /note="Guanylate cyclase 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT REGION 822..861
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 842..856
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1019 AA; 111990 MW; C28F8B3D1EAB1F34 CRC64;
MYILVWKEGQ QIRTFQDLEE CGQFQTASNI TDGQIFSINV TPTMSKGGET GETQLRRLMY
LSASTEPEKC NAEYLADMAH VATLRNKQIG VSGFLLYSSP FFFQVIEGTD EDLDFLFAKI
SADPRHERCI VLANGPCTGR MYGEWHMKDS HIDNITKHPA IKTILFQIAR SFSSMWSYLP
KNAANMLLLG KNPNKQAPEP MSVVVTFIYL VEFSSILAHP GLTEQCADIL AAFVDACVRN
VEGTGGQVAK FITGICMAYW PINRAEDALV GLQQLSEDLA ELRSQQPPGS ALSLIYSRCG
VHYGRALLCN AGFRKADFTL LGDCINTASR ITSLSVKLKV PLLLSFEVRC LLGDEMREEL
ESSGLHKVKG RDKPVQVYQF NAPELDSAMV RAKIEQFNPG RYRALCPVKP YESLHPAQRP
PIFDDTPREN QPKLSQVQRR DSLVDRLSLI AKLAFPSSMM AGGEGQLITL TYISQAAHPM
SRLDLASIQR IAFARNESSN ITGSLLYVSG LFVQTLEGPK GAVVSLYLKI RQDKRHKDVV
AVFMAPIDER VYGSPLDMTS ATEEMLATFP PLQDVLSQLA KSFISLETYV PSTVVRYLTA
GNNPRNLQPV SVEVVMLATD ICSFTPLSEK CSLTEVWTIC NTFIDACTSA ICNEGGEVIK
LIGDCVTAYF PPTGADNAVH ACQEIVSFCA QLRDAFHDVL DCRSVVACGV GLDFGQVIMA
QCGSLGMTEF VVAGEVSARV MEVEALTREA GRAIVITEPV ADRLSPKLRD TGIVPCQEGV
DGVPCYGILG PEWELDVATI KKNIYGFHDA RALAAMKKVD DGTNAPGRGA PAGGIPSSPK
VRPPGRTNSV SSYTPDPNEA LDPRMAESVF LDMCHQRGDT ANNSIAVKLR QAANDDRLDL
GRMLQGPHEL MPVMQAIKHL TNLRMLNMSD NFVDDNNVGE LVESCIPMRS LQVLDLSNNP
GLTKVIALKR LIKHNTQVRE ILLNGTRIAP TEQRKLQSSM NVNRLCASTD LKGSHKYEH
