PD2R_MOUSE
ID PD2R_MOUSE Reviewed; 357 AA.
AC P70263; A2RSY4; Q8CCM3;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Prostaglandin D2 receptor;
DE Short=PGD receptor;
DE Short=PGD2 receptor;
DE AltName: Full=Prostanoid DP receptor;
GN Name=Ptgdr;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Olfactory bulb;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv; TISSUE=Lung;
RX PubMed=7972033; DOI=10.1073/pnas.91.23.11192;
RA Hirata M., Kakizuka A., Aizawa M., Ushikubi F., Narumiya S.;
RT "Molecular characterization of a mouse prostaglandin D receptor and
RT functional expression of the cloned gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:11192-11196(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23624557; DOI=10.1038/ni.2586;
RA Taketomi Y., Ueno N., Kojima T., Sato H., Murase R., Yamamoto K.,
RA Tanaka S., Sakanaka M., Nakamura M., Nishito Y., Kawana M., Kambe N.,
RA Ikeda K., Taguchi R., Nakamizo S., Kabashima K., Gelb M.H., Arita M.,
RA Yokomizo T., Nakamura M., Watanabe K., Hirai H., Nakamura M., Okayama Y.,
RA Ra C., Aritake K., Urade Y., Morimoto K., Sugimoto Y., Shimizu T.,
RA Narumiya S., Hara S., Murakami M.;
RT "Mast cell maturation is driven via a group III phospholipase A2-
RT prostaglandin D2-DP1 receptor paracrine axis.";
RL Nat. Immunol. 14:554-563(2013).
CC -!- FUNCTION: Receptor for prostaglandin D2 (PGD2). The activity of this
CC receptor is mainly mediated by G(s) proteins that stimulate adenylate
CC cyclase, resulting in an elevation of intracellular cAMP. A
CC mobilization of calcium is also observed, but without formation of
CC inositol 1,4,5-trisphosphate (By similarity). Involved in PLA2G3-
CC dependent maturation of mast cells. PLA2G3 is secreted by immature mast
CC cells and acts on nearby fibroblasts upstream to PTDGS to synthesize
CC PGD2, which in turn promotes mast cell maturation and degranulation via
CC PTGDR (PubMed:23624557). {ECO:0000250|UniProtKB:Q9R261,
CC ECO:0000269|PubMed:23624557}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Most abundantly expressed in the ileum, followed by
CC lung, stomach and uterus.
CC -!- DISRUPTION PHENOTYPE: Mutant mice show decreased susceptibility to
CC passive cutaneous anaphylaxis associated with decreased mast cell
CC degranulation. {ECO:0000269|PubMed:23624557}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AK032488; BAC27895.1; -; mRNA.
DR EMBL; D29765; BAA06168.1; -; Genomic_DNA.
DR EMBL; BC132297; AAI32298.1; -; mRNA.
DR CCDS; CCDS26971.1; -.
DR PIR; I59269; I59269.
DR PIR; I80042; I80042.
DR RefSeq; NP_032988.3; NM_008962.4.
DR AlphaFoldDB; P70263; -.
DR SMR; P70263; -.
DR IntAct; P70263; 1.
DR STRING; 10090.ENSMUSP00000093653; -.
DR BindingDB; P70263; -.
DR ChEMBL; CHEMBL3933; -.
DR GuidetoPHARMACOLOGY; 338; -.
DR GlyGen; P70263; 2 sites.
DR PhosphoSitePlus; P70263; -.
DR PaxDb; P70263; -.
DR PRIDE; P70263; -.
DR ProteomicsDB; 287897; -.
DR Antibodypedia; 10764; 218 antibodies from 28 providers.
DR DNASU; 19214; -.
DR Ensembl; ENSMUST00000095959; ENSMUSP00000093653; ENSMUSG00000071489.
DR GeneID; 19214; -.
DR KEGG; mmu:19214; -.
DR UCSC; uc007ter.1; mouse.
DR CTD; 5729; -.
DR MGI; MGI:102966; Ptgdr.
DR VEuPathDB; HostDB:ENSMUSG00000071489; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244902; -.
DR HOGENOM; CLU_045991_0_2_1; -.
DR InParanoid; P70263; -.
DR OMA; FRIFFHK; -.
DR OrthoDB; 972015at2759; -.
DR PhylomeDB; P70263; -.
DR TreeFam; TF324982; -.
DR Reactome; R-MMU-391908; Prostanoid ligand receptors.
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR BioGRID-ORCS; 19214; 2 hits in 71 CRISPR screens.
DR PRO; PR:P70263; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; P70263; protein.
DR Bgee; ENSMUSG00000071489; Expressed in lumbar dorsal root ganglion and 34 other tissues.
DR Genevisible; P70263; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0004956; F:prostaglandin D receptor activity; IDA:MGI.
DR GO; GO:0001785; F:prostaglandin J receptor activity; IDA:MGI.
DR GO; GO:0046085; P:adenosine metabolic process; IMP:MGI.
DR GO; GO:0071799; P:cellular response to prostaglandin D stimulus; IMP:MGI.
DR GO; GO:0006954; P:inflammatory response; IMP:MGI.
DR GO; GO:0030238; P:male sex determination; IMP:MGI.
DR GO; GO:0043303; P:mast cell degranulation; IEA:UniProtKB-KW.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0030431; P:sleep; IMP:MGI.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000376; Pglndn_D_rcpt.
DR InterPro; IPR008365; Prostanoid_rcpt.
DR PANTHER; PTHR11866; PTHR11866; 1.
DR PANTHER; PTHR11866:SF14; PTHR11866:SF14; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR01788; PROSTANOIDR.
DR PRINTS; PR00854; PRSTNOIDDPR.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Mast cell degranulation; Membrane; Receptor; Reference proteome;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..357
FT /note="Prostaglandin D2 receptor"
FT /id="PRO_0000070048"
FT TOPO_DOM 1..20
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..41
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..106
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..149
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 171..194
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 283..306
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..327
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 328..357
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 2
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 104..182
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 75
FT /note="K -> N (in Ref. 1; BAC27895)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 357 AA; 40005 MW; 91CBFF7A5936EB05 CRC64;
MNESYRCQTS TWVERGSSAT MGAVLFGAGL LGNLLALVLL ARSGLGSCRP GPLHPPPSVF
YVLVCGLTVT DLLGKCLISP MVLAAYAQNQ SLKELLPASG NQLCETFAFL MSFFGLASTL
QLLAMAVECW LSLGHPFFYQ RHVTLRRGVL VAPVVAAFCL AFCALPFAGF GKFVQYCPGT
WCFIQMIHKE RSFSVIGFSV LYSSLMALLV LATVVCNLGA MYNLYDMHRR QRHYPHRCSR
DRAQSGSDYR HGSLHPLEEL DHFVLLALMT VLFTMCSLPL IYRAYYGAFK LENKAEGDSE
DLQALRFLSV ISIVDPWIFI IFRTSVFRML FHKVFTRPLI YRNWSSHSQQ SNVESTL
