ID   PDAD2_METAC             Reviewed;         183 AA.
AC   Q8TKB4;
DT   11-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Pyruvoyl-dependent arginine decarboxylase 2;
DE            Short=PvlArgDC 2;
DE            EC=4.1.1.19;
DE   Contains:
DE     RecName: Full=Pyruvoyl-dependent arginine decarboxylase 2 subunit beta;
DE   Contains:
DE     RecName: Full=Pyruvoyl-dependent arginine decarboxylase 2 subunit alpha;
GN   Name=pdaD2; OrderedLocusNames=MA_3496;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + L-arginine = agmatine + CO2; Xref=Rhea:RHEA:17641,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:32682,
CC         ChEBI:CHEBI:58145; EC=4.1.1.19;
CC   -!- COFACTOR:
CC       Name=pyruvate; Xref=ChEBI:CHEBI:15361; Evidence={ECO:0000250};
CC       Note=Binds 1 pyruvoyl group covalently per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the PdaD family. {ECO:0000305}.
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DR   EMBL; AE010299; AAM06861.1; -; Genomic_DNA.
DR   RefSeq; WP_011023416.1; NC_003552.1.
DR   AlphaFoldDB; Q8TKB4; -.
DR   SMR; Q8TKB4; -.
DR   STRING; 188937.MA_3496; -.
DR   EnsemblBacteria; AAM06861; AAM06861; MA_3496.
DR   GeneID; 1475390; -.
DR   KEGG; mac:MA_3496; -.
DR   HOGENOM; CLU_114389_0_0_2; -.
DR   InParanoid; Q8TKB4; -.
DR   OMA; SEHHSFG; -.
DR   OrthoDB; 98606at2157; -.
DR   PhylomeDB; Q8TKB4; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0008792; F:arginine decarboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006527; P:arginine catabolic process; IEA:InterPro.
DR   Gene3D; 3.50.20.10; -; 1.
DR   HAMAP; MF_01404; PvlArgDC; 1.
DR   InterPro; IPR016104; Pyr-dep_his/arg-deCO2ase.
DR   InterPro; IPR016105; Pyr-dep_his/arg-deCO2ase_sand.
DR   InterPro; IPR002724; Pyruvoyl-dep_arg_deCO2ase.
DR   PANTHER; PTHR40438; PTHR40438; 1.
DR   Pfam; PF01862; PvlArgDC; 1.
DR   PIRSF; PIRSF005216; Pyruvoyl-dep_arg_deCO2ase; 1.
DR   SFLD; SFLDG01170; Pyruvoyl-dependent_arginine_de; 1.
DR   SUPFAM; SSF56271; SSF56271; 1.
DR   TIGRFAMs; TIGR00286; TIGR00286; 1.
PE   3: Inferred from homology;
KW   Decarboxylase; Lyase; Pyruvate; Reference proteome.
FT   CHAIN           1..40
FT                   /note="Pyruvoyl-dependent arginine decarboxylase 2 subunit
FT                   beta"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000023312"
FT   CHAIN           41..183
FT                   /note="Pyruvoyl-dependent arginine decarboxylase 2 subunit
FT                   alpha"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000023313"
FT   SITE            40..41
FT                   /note="Cleavage (non-hydrolytic)"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         41
FT                   /note="Pyruvic acid (Ser)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   183 AA;  20138 MW;  F4E8FE02CC5923B1 CRC64;
     MIPRKAFLTK GTGVHKDRLA SFELALRDAK IEKYNLVSVS SILPPNCKLV TREEGLSELK
     PGAIVHCVLA RNDTNEPHRL MASAIGTAVP VNEDNYGYIS EHHSFGEEEI IAGEYAEDLA
     ATMLATTLGI EFNAEMAWHE REQVYKASGH IFDTFHICQT AAGDKNGKWT TVVAAMVFVT
     SKC