PDC2_YEAST
ID PDC2_YEAST Reviewed; 925 AA.
AC P32896; D6VS68; E9PAG9;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Protein PDC2;
GN Name=PDC2; OrderedLocusNames=YDR081C; ORFNames=D4451;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8264540; DOI=10.1007/bf00279908;
RA Hohmann S.;
RT "Characterisation of PDC2, a gene necessary for high level expression of
RT pyruvate decarboxylase structural genes in Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 241:657-666(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Raghuram V., Lobo Z., Maitra P.K.;
RT "Cloning and characterization of PDC2, a gene essential for the synthesis
RT of pyruvate decarboxylase in Saccharomyces cerevisiae.";
RL Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7483840; DOI=10.1002/yea.320110708;
RA Coster F., Jonniaux J.-L., Goffeau A.;
RT "Analysis of a 32.8 kb segment of yeast chromosome IV reveals 21 open
RT reading frames, including TPS2, PPH3, RAD55, SED1, PDC2, AFR1, SSS1, SLU7
RT and a tRNA for arginine.";
RL Yeast 11:673-679(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Essential for the synthesis of pyruvate decarboxylase. May be
CC important for a high basal level of PDC gene expression or play a
CC positive role in the autoregulation control of PDC1 and PDC5.
CC -!- INTERACTION:
CC P32896; Q07471: THI3; NbExp=3; IntAct=EBI-13004, EBI-19209;
CC -!- MISCELLANEOUS: Present with 572 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X65608; CAA46558.1; -; Genomic_DNA.
DR EMBL; L19880; AAA34845.1; -; Genomic_DNA.
DR EMBL; X82086; CAA57608.1; -; Genomic_DNA.
DR EMBL; Z46796; CAA86803.1; -; Genomic_DNA.
DR EMBL; Z74377; CAA98900.1; -; Genomic_DNA.
DR EMBL; Z74378; CAA98901.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11928.1; -; Genomic_DNA.
DR PIR; S48768; S48768.
DR RefSeq; NP_010366.1; NM_001180389.1.
DR AlphaFoldDB; P32896; -.
DR BioGRID; 32137; 390.
DR IntAct; P32896; 6.
DR MINT; P32896; -.
DR STRING; 4932.YDR081C; -.
DR iPTMnet; P32896; -.
DR MaxQB; P32896; -.
DR PaxDb; P32896; -.
DR PRIDE; P32896; -.
DR EnsemblFungi; YDR081C_mRNA; YDR081C; YDR081C.
DR GeneID; 851654; -.
DR KEGG; sce:YDR081C; -.
DR SGD; S000002488; PDC2.
DR VEuPathDB; FungiDB:YDR081C; -.
DR eggNOG; KOG3105; Eukaryota.
DR GeneTree; ENSGT00940000175769; -.
DR HOGENOM; CLU_009537_0_0_1; -.
DR InParanoid; P32896; -.
DR OMA; VWHRIPS; -.
DR BioCyc; YEAST:G3O-29686-MON; -.
DR PRO; PR:P32896; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P32896; protein.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:2001172; P:positive regulation of glycolytic fermentation to ethanol; IMP:SGD.
DR GO; GO:0090180; P:positive regulation of thiamine biosynthetic process; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:SGD.
DR InterPro; IPR004875; DDE_SF_endonuclease_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR006600; HTH_CenpB_DNA-bd_dom.
DR Pfam; PF03184; DDE_1; 1.
DR Pfam; PF03221; HTH_Tnp_Tc5; 1.
DR SMART; SM00674; CENPB; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51253; HTH_CENPB; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome.
FT CHAIN 1..925
FT /note="Protein PDC2"
FT /id="PRO_0000126137"
FT DOMAIN 63..138
FT /note="HTH CENPB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00583"
FT REGION 510..596
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 674..693
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 904..925
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 113
FT /note="H -> T (in Ref. 1; CAA46558 and 2; AAA34845)"
FT /evidence="ECO:0000305"
FT CONFLICT 174
FT /note="D -> I (in Ref. 1; CAA46558 and 2; AAA34845)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 925 AA; 103944 MW; 579FFF99A944E686 CRC64;
MLSIQQRYNI CLMAERHPKW TQLELAKWAY ETFQLPKIPS QGTISRLLAR KSTYMNCKEH
EKDANRLRKP NNLLVRKILQ EWISQSLWNG IPITSPIIQD TAQAVWHRIP AEHREGNGSF
SYKWISNFLS KMDVNISVLD EELPKTPKVW TFEERDVLKA YFSKIPPKDL FTLDEAFLSY
NLPLDYAQYE ASSIQRRIEV ATVMLCSNLD GSEKLKPVVV GKYDSYKSFR NYFPNEPNDP
VSQSMLGTKM AKKFDISYHS NRKAWLTSNL FHNWLVRWDK RLVAVNRKIW IVLDDSCCHR
IINLRLQNIK LVYTSSNSKF LPFNWGVWDE FKTRYRIQQY QALIDLQNRI SKNIQNKNKS
ERNECIPNGK KCLISFEQSQ LTMSNAFKFI KKAWDDIPVD AIKANWKSSG LLPPEMIHLN
ENVSMAFKKN EVLESVLNRL CDEYYCVKKW EYEMLLDLNI ENKNTNFLST EELVESAIVE
PCEPDFDTAP KGNEVHDDNF DVSVFANEDD NNQNHLSMSQ ASHNPDYNSN HSNNAIENTN
NRGSNNNNNN NGSSNNINDN DSSVKYLQQN TVDNSTKTGN PGQPNISSME SQRNSSTTDL
VVDGNYDVNF NGLLNDPYNT MKQPGPLDYN VSTLIDKPNL FLSPDLDLST VGVDMQLPSS
EYFSEVFSSA IRNNEKAASD QNKSTDELPS STAMANSNSI TTALLESRNQ AQPFDVPHMN
GLLSDTSKSG HSVNSSNAIS QNSLNNFQHN SASVAEASSP SITPSPVAIN STGAPARSII
SAPIDSNSSA SSPSALEHLE GAVSGMSPSS TTILSNLQTN INIAKSLSTI MKHAESNEIS
LTKETINELN FNYLTLLKRI KKTRKQLNSE SIKINSKNAQ DHLETLLSGA AAAAATSANN
LDLPTGGSNL PDSNNLHLPG NTGFF
