PDCB2_ARATH
ID PDCB2_ARATH Reviewed; 194 AA.
AC Q9SD84; Q8LBE9;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=PLASMODESMATA CALLOSE-BINDING PROTEIN 2;
DE Short=AtPDCB2;
DE AltName: Full=Glucan endo-1,3-beta-glucosidase-like protein 3;
DE Flags: Precursor;
GN Name=PDCB2; OrderedLocusNames=At5g08000; ORFNames=F13G24.200;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, NOMENCLATURE, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION BY HEAT, AND GPI-ANCHOR.
RX PubMed=19223515; DOI=10.1105/tpc.108.060145;
RA Simpson C., Thomas C., Findlay K., Bayer E., Maule A.J.;
RT "An Arabidopsis GPI-anchor plasmodesmal neck protein with callose binding
RT activity and potential to regulate cell-to-cell trafficking.";
RL Plant Cell 21:581-594(2009).
CC -!- FUNCTION: Able to bind (1->3)-beta-D-glucans (laminarin).
CC {ECO:0000269|PubMed:19223515}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19223515};
CC Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:19223515}. Cell junction,
CC plasmodesma {ECO:0000269|PubMed:19223515}.
CC -!- TISSUE SPECIFICITY: Expressed in the shoot apical region and in young
CC leaves but also detected in the laminar and vasculature of mature
CC leaves. {ECO:0000269|PubMed:19223515}.
CC -!- INDUCTION: Down-regulated by heat treatment.
CC {ECO:0000269|PubMed:19223515}.
CC -!- PTM: Contains two additional disulfide bonds. {ECO:0000250}.
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DR EMBL; AL133421; CAB62612.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91233.1; -; Genomic_DNA.
DR EMBL; BT003716; AAO39944.1; -; mRNA.
DR EMBL; AK118578; BAC43178.1; -; mRNA.
DR EMBL; AY087253; AAM64809.1; -; mRNA.
DR PIR; T45625; T45625.
DR RefSeq; NP_196417.1; NM_120882.4.
DR AlphaFoldDB; Q9SD84; -.
DR SMR; Q9SD84; -.
DR IntAct; Q9SD84; 1.
DR STRING; 3702.AT5G08000.1; -.
DR CAZy; CBM43; Carbohydrate-Binding Module Family 43.
DR TCDB; 1.I.2.1.1; the plant plasmodesmata (ppd) family.
DR PaxDb; Q9SD84; -.
DR PRIDE; Q9SD84; -.
DR EnsemblPlants; AT5G08000.1; AT5G08000.1; AT5G08000.
DR GeneID; 830694; -.
DR Gramene; AT5G08000.1; AT5G08000.1; AT5G08000.
DR KEGG; ath:AT5G08000; -.
DR Araport; AT5G08000; -.
DR TAIR; locus:2142778; AT5G08000.
DR eggNOG; ENOG502RYRZ; Eukaryota.
DR HOGENOM; CLU_031666_1_0_1; -.
DR InParanoid; Q9SD84; -.
DR OMA; PTGPCYS; -.
DR PhylomeDB; Q9SD84; -.
DR PRO; PR:Q9SD84; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9SD84; baseline and differential.
DR Genevisible; Q9SD84; AT.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0046658; C:anchored component of plasma membrane; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; IDA:TAIR.
DR GO; GO:0001872; F:(1->3)-beta-D-glucan binding; IDA:TAIR.
DR GO; GO:0030247; F:polysaccharide binding; IDA:TAIR.
DR GO; GO:0009408; P:response to heat; IEP:UniProtKB.
DR InterPro; IPR012946; X8.
DR InterPro; IPR044788; X8_dom_prot.
DR PANTHER; PTHR31044; PTHR31044; 1.
DR Pfam; PF07983; X8; 1.
DR SMART; SM00768; X8; 1.
PE 1: Evidence at protein level;
KW Cell junction; Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor;
KW Lipoprotein; Membrane; Reference proteome; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..171
FT /note="PLASMODESMATA CALLOSE-BINDING PROTEIN 2"
FT /id="PRO_0000254003"
FT PROPEP 172..194
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000254004"
FT REGION 106..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 171
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 154
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 22..84
FT /evidence="ECO:0000250"
FT CONFLICT 3
FT /note="P -> A (in Ref. 5; AAM64809)"
FT /evidence="ECO:0000305"
FT CONFLICT 17
FT /note="T -> S (in Ref. 5; AAM64809)"
FT /evidence="ECO:0000305"
FT CONFLICT 143
FT /note="S -> T (in Ref. 5; AAM64809)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 194 AA; 19900 MW; 3C0A089C3452662A CRC64;
MAPLVLYLLT LLMAGHTSAS WCVCKTGLSD SVLQKTLDYA CGNGADCNPT HPKGSCFNPD
NVRAHCNYAV NSFFQKKGQA SESCNFTGTA TLTTTDPSYT GCAFPSSASG SSGSGSTTVT
PGKNSPKGSN SITTFPGGNS PYSGTPSTGL LGGNITDATG TGLNPDYSTE SSGFALYYSN
NLLLTGFCSL VMML
