PDCB4_ARATH
ID PDCB4_ARATH Reviewed; 222 AA.
AC Q93V72; Q67ZF7; Q8GWY5; Q8LE41; Q9C982;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=PLASMODESMATA CALLOSE-BINDING PROTEIN 4;
DE Short=AtPDCB4;
DE Flags: Precursor;
GN Name=PDCB4; OrderedLocusNames=At1g69295; ORFNames=F23O10.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY, NOMENCLATURE, SUBCELLULAR LOCATION, AND GPI-ANCHOR.
RX PubMed=19223515; DOI=10.1105/tpc.108.060145;
RA Simpson C., Thomas C., Findlay K., Bayer E., Maule A.J.;
RT "An Arabidopsis GPI-anchor plasmodesmal neck protein with callose binding
RT activity and potential to regulate cell-to-cell trafficking.";
RL Plant Cell 21:581-594(2009).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19223515};
CC Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:19223515}. Cell junction,
CC plasmodesma {ECO:0000269|PubMed:19223515}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q93V72-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q93V72-2; Sequence=VSP_034544;
CC -!- PTM: Contains two additional disulfide bonds. {ECO:0000250}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52501.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g69290 and At1g69295.; Evidence={ECO:0000305};
CC Sequence=BAD43464.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAD43923.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC018364; AAG52501.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34907.1; -; Genomic_DNA.
DR EMBL; AK118559; BAC43160.1; -; mRNA.
DR EMBL; AF370153; AAK43968.1; -; mRNA.
DR EMBL; AF412078; AAL06531.1; -; mRNA.
DR EMBL; AY056376; AAL08232.1; -; mRNA.
DR EMBL; AY059094; AAL15200.1; -; mRNA.
DR EMBL; AK175349; BAD43112.1; -; mRNA.
DR EMBL; AK175595; BAD43358.1; -; mRNA.
DR EMBL; AK175601; BAD43364.1; -; mRNA.
DR EMBL; AK175695; BAD43458.1; -; mRNA.
DR EMBL; AK175701; BAD43464.1; ALT_FRAME; mRNA.
DR EMBL; AK175748; BAD43511.1; -; mRNA.
DR EMBL; AK175773; BAD43536.1; -; mRNA.
DR EMBL; AK175835; BAD43598.1; -; mRNA.
DR EMBL; AK175881; BAD43644.1; -; mRNA.
DR EMBL; AK175916; BAD43679.1; -; mRNA.
DR EMBL; AK176017; BAD43780.1; -; mRNA.
DR EMBL; AK176076; BAD43839.1; -; mRNA.
DR EMBL; AK176160; BAD43923.1; ALT_FRAME; mRNA.
DR EMBL; AK176590; BAD44353.1; -; mRNA.
DR EMBL; AK175814; BAD43577.1; -; mRNA.
DR EMBL; AK175832; BAD43595.1; -; mRNA.
DR EMBL; AY085640; AAM62861.1; -; mRNA.
DR PIR; A96717; A96717.
DR RefSeq; NP_564957.1; NM_105597.3. [Q93V72-1]
DR AlphaFoldDB; Q93V72; -.
DR SMR; Q93V72; -.
DR STRING; 3702.AT1G69295.1; -.
DR CAZy; CBM43; Carbohydrate-Binding Module Family 43.
DR TCDB; 1.I.2.1.1; the plant plasmodesmata (ppd) family.
DR PaxDb; Q93V72; -.
DR PRIDE; Q93V72; -.
DR ProteomicsDB; 236365; -. [Q93V72-1]
DR EnsemblPlants; AT1G69295.1; AT1G69295.1; AT1G69295. [Q93V72-1]
DR GeneID; 843261; -.
DR Gramene; AT1G69295.1; AT1G69295.1; AT1G69295. [Q93V72-1]
DR KEGG; ath:AT1G69295; -.
DR Araport; AT1G69295; -.
DR TAIR; locus:505006212; AT1G69295.
DR eggNOG; ENOG502S0BH; Eukaryota.
DR HOGENOM; CLU_031666_1_0_1; -.
DR OMA; CICNSAL; -.
DR OrthoDB; 1484359at2759; -.
DR PRO; PR:Q93V72; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q93V72; baseline and differential.
DR Genevisible; Q93V72; AT.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; IDA:TAIR.
DR GO; GO:0001872; F:(1->3)-beta-D-glucan binding; ISS:TAIR.
DR GO; GO:0030247; F:polysaccharide binding; ISS:TAIR.
DR InterPro; IPR012946; X8.
DR InterPro; IPR044788; X8_dom_prot.
DR PANTHER; PTHR31044; PTHR31044; 1.
DR Pfam; PF07983; X8; 1.
DR SMART; SM00768; X8; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell junction; Cell membrane; Disulfide bond;
KW Glycoprotein; GPI-anchor; Lipoprotein; Membrane; Reference proteome;
KW Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..197
FT /note="PLASMODESMATA CALLOSE-BINDING PROTEIN 4"
FT /id="PRO_0000342741"
FT PROPEP 198..222
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000430193"
FT REGION 88..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 197
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 22..83
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..164
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074, ECO:0000303|Ref.5"
FT /id="VSP_034544"
FT CONFLICT 101
FT /note="N -> S (in Ref. 6; AAM62861)"
FT /evidence="ECO:0000305"
FT CONFLICT 119
FT /note="T -> S (in Ref. 6; AAM62861)"
FT /evidence="ECO:0000305"
FT CONFLICT 209
FT /note="V -> L (in Ref. 6; AAM62861)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 222 AA; 22076 MW; EB3089D9C410E13F CRC64;
MSVLLPLCLI ISMFTYSNAA YCLCKEGNEQ VLQKAIDYAC GNGADCTQIQ PTGACYQPNT
VKNHCDVAVN SYYQKKASSG ATCDFNGAAS PSTTPPSTAS NCLTGSSSSG TPTTGTPTTG
TPTSGTPTTG TPTTGTPTTG TPTSGTPTSG FPNTGTPNTG TNTGMPNSNG MPTSSSSSVF
PGTTLGPTGS GGLGDPNAGE KLSVRTNTVV FLLTGVAAML VI
