ASPP_BACSU
ID ASPP_BACSU Reviewed; 520 AA.
AC O07002;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Aspartate-proton symporter;
DE AltName: Full=L-aspartate transporter;
GN Name=yveA; OrderedLocusNames=BSU34470;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Denizot F.;
RL Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP INDUCTION.
RX PubMed=11739774; DOI=10.1099/00221287-147-12-3413;
RA Pereira Y., Petit-Glatron M.-F., Chambert R.;
RT "yveB, encoding endolevanase LevB, is part of the sacB-yveB-yveA
RT levansucrase tricistronic operon in Bacillus subtilis.";
RL Microbiology 147:3413-3419(2001).
RN [4]
RP CHARACTERIZATION.
RC STRAIN=168;
RX PubMed=12730183; DOI=10.1128/jb.185.10.3218-3222.2003;
RA Lorca G., Winnen B., Saier M.H. Jr.;
RT "Identification of the L-aspartate transporter in Bacillus subtilis.";
RL J. Bacteriol. 185:3218-3222(2003).
CC -!- FUNCTION: Uptake of L-aspartate with the concomitant import of a
CC proton. Can also transport aspartate hydroxamate and L-glutamate with
CC lower affinity and efficiency.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- INDUCTION: Induced by sucrose. {ECO:0000269|PubMed:11739774}.
CC -!- MISCELLANEOUS: Allows enhanced growth with L-aspartate as a sole source
CC nitrogen source.
CC -!- MISCELLANEOUS: Inhibited by carbonyl cyanide m-chlorophenylhydrazone
CC and carbonyl cyanide 4-trifluoro-methoxyphenylhydrazone.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. AGT (TC 2.A.3.11) family. {ECO:0000305}.
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DR EMBL; Z94043; CAB08050.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15452.1; -; Genomic_DNA.
DR PIR; D70035; D70035.
DR RefSeq; NP_391327.1; NC_000964.3.
DR RefSeq; WP_003228228.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; O07002; -.
DR SMR; O07002; -.
DR STRING; 224308.BSU34470; -.
DR TCDB; 2.A.3.11.1; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; O07002; -.
DR PRIDE; O07002; -.
DR EnsemblBacteria; CAB15452; CAB15452; BSU_34470.
DR GeneID; 938612; -.
DR KEGG; bsu:BSU34470; -.
DR PATRIC; fig|224308.179.peg.3734; -.
DR eggNOG; COG0531; Bacteria.
DR InParanoid; O07002; -.
DR OMA; PCTATIV; -.
DR PhylomeDB; O07002; -.
DR BioCyc; BSUB:BSU34470-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR InterPro; IPR002293; AA/rel_permease1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..520
FT /note="Aspartate-proton symporter"
FT /id="PRO_0000054283"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 402..422
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..480
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 520 AA; 56989 MW; 0583CB56FE6D37D9 CRC64;
MSKQGNFQKS MSLFDLILIG MGAIFGSAWL FAVSNVASKA GPSGAFSWIL GGAIILLIGL
VYAELGAALP RTGGIIRYPV YSHGHLVGYL ISFVTIVAYT SLISIEVTAV RQYVAYWFPG
LTIKGSDSPT ISGWILQFAL LCLFFLLNYW SVKTFAKANF IISIFKYIVP ITIIIVLIFH
FQPENLSVQG FAPFGFTGIQ AAISTGGVMF AYLGLHPIVS VAGEVQNPKR NIPIALIICI
IVSTIIYTVL QVTFIGAIPT ETLKHGWPAI GREFSLPFKD IAVMLGLGWL ATLVILDAIL
SPGGNGNIFM NTTSRLVYAW ARNGTLFGIF SKVNKDTGTP RASLWLSFAL SIFWTLPFPS
WNALVNVCSV ALILSYAIAP ISSAALRVNA KDLNRPFYLK GMSIIGPLSF IFTAFIVYWS
GWKTVSWLLG SQLVMFLIYL CFSKYTPKED VSLAQQLKSA WWLIGFYIMM LIFSYIGSFG
HGLGIISNPV DLILVAIGSL AIYYWAKYTG LPKAAIDYDK
