ASPR1_ORYSJ
ID ASPR1_ORYSJ Reviewed; 509 AA.
AC Q42456; Q688W6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Aspartic proteinase oryzasin-1;
DE EC=3.4.23.-;
DE Flags: Precursor;
GN OrderedLocusNames=Os05g0567100, LOC_Os05g49200; ORFNames=OJ1781_H11.11;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Seed;
RX PubMed=7556174; DOI=10.1111/j.1432-1033.1995.tb20783.x;
RA Asakura T., Watanabe H., Abe K., Arai S.;
RT "Rice aspartic proteinase, oryzasin, expressed during seed ripening and
RT germination, has a gene organization distinct from those of animal and
RT microbial aspartic proteinases.";
RL Eur. J. Biochem. 232:77-83(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Involved in the breakdown of propeptides of storage proteins
CC in protein-storage vacuoles. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Seed ripening and germination.
CC -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D32144; BAA06875.1; -; mRNA.
DR EMBL; D32165; BAA06876.1; -; Genomic_DNA.
DR EMBL; AC120986; AAU10663.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS95382.1; -; Genomic_DNA.
DR PIR; S66516; S66516.
DR RefSeq; XP_015639571.1; XM_015784085.1.
DR AlphaFoldDB; Q42456; -.
DR SMR; Q42456; -.
DR STRING; 4530.OS05T0567100-02; -.
DR MEROPS; A01.020; -.
DR PaxDb; Q42456; -.
DR PRIDE; Q42456; -.
DR EnsemblPlants; Os05t0567100-01; Os05t0567100-01; Os05g0567100.
DR EnsemblPlants; Os05t0567100-02; Os05t0567100-02; Os05g0567100.
DR GeneID; 4339639; -.
DR Gramene; Os05t0567100-01; Os05t0567100-01; Os05g0567100.
DR Gramene; Os05t0567100-02; Os05t0567100-02; Os05g0567100.
DR KEGG; osa:4339639; -.
DR eggNOG; KOG1339; Eukaryota.
DR InParanoid; Q42456; -.
DR OMA; GVECANL; -.
DR OrthoDB; 1619495at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR ExpressionAtlas; Q42456; baseline and differential.
DR Genevisible; Q42456; OS.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR CDD; cd06098; phytepsin; 1.
DR Gene3D; 2.40.70.10; -; 2.
DR InterPro; IPR001461; Aspartic_peptidase_A1.
DR InterPro; IPR001969; Aspartic_peptidase_AS.
DR InterPro; IPR033121; PEPTIDASE_A1.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR InterPro; IPR033869; Phytepsin.
DR InterPro; IPR007856; SapB_1.
DR InterPro; IPR008138; SapB_2.
DR InterPro; IPR011001; Saposin-like.
DR InterPro; IPR008139; SaposinB_dom.
DR PANTHER; PTHR47966; PTHR47966; 1.
DR Pfam; PF00026; Asp; 1.
DR Pfam; PF05184; SapB_1; 1.
DR Pfam; PF03489; SapB_2; 1.
DR PRINTS; PR00792; PEPSIN.
DR SMART; SM00741; SapB; 1.
DR SUPFAM; SSF47862; SSF47862; 1.
DR SUPFAM; SSF50630; SSF50630; 1.
DR PROSITE; PS00141; ASP_PROTEASE; 2.
DR PROSITE; PS51767; PEPTIDASE_A1; 1.
DR PROSITE; PS50015; SAP_B; 2.
PE 2: Evidence at transcript level;
KW Aspartyl protease; Disulfide bond; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Signal; Vacuole; Zymogen.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..67
FT /note="Activation peptide"
FT /evidence="ECO:0000255"
FT /id="PRO_0000025908"
FT CHAIN 68..509
FT /note="Aspartic proteinase oryzasin-1"
FT /id="PRO_0000025909"
FT DOMAIN 85..506
FT /note="Peptidase A1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT DOMAIN 315..420
FT /note="Saposin B-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT ACT_SITE 103
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT ACT_SITE 290
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT CARBOHYD 252
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT CARBOHYD 400
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 116..122
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 281..285
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 320..414
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 345..386
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 351..383
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 428..465
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT CONFLICT 25
FT /note="A -> E (in Ref. 1; BAA06875/BAA06876)"
FT /evidence="ECO:0000305"
FT CONFLICT 443
FT /note="G -> A (in Ref. 1; BAA06875/BAA06876)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 509 AA; 54074 MW; 6316EB933C5749C2 CRC64;
MGTRSVALVL LAAVLLQALL PASAAEGLVR IALKKRPIDE NSRVAARLSG EEGARRLGLR
GANSLGGGGG EGDIVALKNY MNAQYFGEIG VGTPPQKFTV IFDTGSSNLW VPSAKCYFSI
ACFFHSRYKS GQSSTYQKNG KPAAIQYGTG SIAGFFSEDS VTVGDLVVKD QEFIEATKEP
GLTFMVAKFD GILGLGFQEI SVGDAVPVWY KMVEQGLVSE PVFSFWFNRH SDEGEGGEIV
FGGMDPSHYK GNHTYVPVSQ KGYWQFEMGD VLIGGKTTGF CASGCSAIAD SGTSLLAGPT
AIITEINEKI GATGVVSQEC KTVVSQYGQQ ILDLLLAETQ PSKICSQVGL CTFDGKHGVS
AGIKSVVDDE AGESNGLQSG PMCNACEMAV VWMQNQLAQN KTQDLILNYI NQLCDKLPSP
MGESSVDCGS LASMPEISFT IGGKKFALKP EEYILKVGEG AAAQCISGFT AMDIPPPRGP
LWILGDVFMG AYHTVFDYGK MRVGFAKSA
