ASPRX_ORYSJ
ID ASPRX_ORYSJ Reviewed; 496 AA.
AC P42211; Q0DKX3; Q75L35; Q9XIV3;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Aspartic proteinase;
DE EC=3.4.23.-;
DE Flags: Precursor;
GN Name=RAP; OrderedLocusNames=Os05g0137400, LOC_Os05g04630;
GN ORFNames=OJ1127_B08.13;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Hashimoto H., Nishi R., Uchimiya H., Kato A.;
RT "Nucleotide sequence of a cDNA encoding aspartic proteinase in rice.";
RL Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 405-496.
RA Tao Y., Li L.;
RT "Chilling sensitive rice partial mRNA for aspartic protease.";
RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the breakdown of propeptides of storage proteins
CC in protein-storage vacuoles. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000305}.
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DR EMBL; D12777; BAA02242.1; -; mRNA.
DR EMBL; AC093490; AAS98423.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF16500.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS92166.1; -; Genomic_DNA.
DR EMBL; AK065206; BAG89416.1; -; mRNA.
DR EMBL; AB028888; BAA78908.1; -; mRNA.
DR PIR; JS0732; JS0732.
DR AlphaFoldDB; P42211; -.
DR SMR; P42211; -.
DR STRING; 4530.OS05T0137400-01; -.
DR PaxDb; P42211; -.
DR PRIDE; P42211; -.
DR EnsemblPlants; Os05t0137400-01; Os05t0137400-01; Os05g0137400.
DR Gramene; Os05t0137400-01; Os05t0137400-01; Os05g0137400.
DR eggNOG; KOG1339; Eukaryota.
DR HOGENOM; CLU_013253_3_1_1; -.
DR InParanoid; P42211; -.
DR OMA; DKSHYTG; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; P42211; OS.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR CDD; cd06098; phytepsin; 1.
DR Gene3D; 2.40.70.10; -; 2.
DR InterPro; IPR001461; Aspartic_peptidase_A1.
DR InterPro; IPR001969; Aspartic_peptidase_AS.
DR InterPro; IPR033121; PEPTIDASE_A1.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR InterPro; IPR033869; Phytepsin.
DR InterPro; IPR007856; SapB_1.
DR InterPro; IPR008138; SapB_2.
DR InterPro; IPR011001; Saposin-like.
DR InterPro; IPR008139; SaposinB_dom.
DR PANTHER; PTHR47966; PTHR47966; 1.
DR Pfam; PF00026; Asp; 1.
DR Pfam; PF05184; SapB_1; 1.
DR Pfam; PF03489; SapB_2; 1.
DR PRINTS; PR00792; PEPSIN.
DR SMART; SM00741; SapB; 2.
DR SUPFAM; SSF47862; SSF47862; 1.
DR SUPFAM; SSF50630; SSF50630; 1.
DR PROSITE; PS00141; ASP_PROTEASE; 2.
DR PROSITE; PS51767; PEPTIDASE_A1; 1.
DR PROSITE; PS50015; SAP_B; 2.
PE 2: Evidence at transcript level;
KW Aspartyl protease; Disulfide bond; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Signal; Vacuole; Zymogen.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..59
FT /note="Activation peptide"
FT /evidence="ECO:0000255"
FT /id="PRO_0000025910"
FT CHAIN 60..496
FT /note="Aspartic proteinase"
FT /id="PRO_0000025911"
FT DOMAIN 77..493
FT /note="Peptidase A1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT DOMAIN 307..407
FT /note="Saposin B-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT ACT_SITE 95
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT ACT_SITE 282
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT CARBOHYD 387
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 108..114
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 273..277
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 312..401
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 337..373
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 343..370
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 415..452
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT CONFLICT 158
FT /note="L -> Q (in Ref. 1; BAA02242)"
FT /evidence="ECO:0000305"
FT CONFLICT 431
FT /note="K -> R (in Ref. 7; BAA78908)"
FT /evidence="ECO:0000305"
FT CONFLICT 492
FT /note="F -> V (in Ref. 7; BAA78908)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 496 AA; 54090 MW; 82F5AE431A833FE0 CRC64;
MAKRHLLLVT TCLWALSCAL LLHASSDGFL RVNLNKKRLD KEDLTAAKLA QQGNRLLKTG
SSDSDPVPLV DYLNTQYYGV IGLGSPPQNF TVIFDTGSSN LWVPSAKCYF SIACYLHSRY
NSKKSSSYKA DGETCKITYG SGAISGFFSK DNVLVGDLVV KNQKFIEATR ETSVTFIIGK
FDGILGLGYP EISVGKAPPI WQSMQEQELL ADDVFSFWLN RDPDASSGGE LVFGGMDPKH
YKGDHTYVPV SRKGYWQFNM GDLLIDGHST GFCAKGCAAI VDSGTSLLAG PTAIVAQVNH
AIGAEGIIST ECKEVVSEYG EMILNLLIAQ TDPQKVCSQV GLCMFDGKRS VSNGIESVVD
KENLGSDAMC SVCEMAVVWI ENQLRENKTK ELILNYANQL CERLPSPNGE STVSCHQISK
MPNLAFTIAN KTFILTPEQY IVKLEQGGQT VCISGFMAFD IPPPRGPLWI LGDVFMGAYH
TVFDFGKDRI GFAKSA
