PDEB_ECOLI
ID PDEB_ECOLI Reviewed; 516 AA.
AC P77473; Q2MBX0;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2001, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Probable cyclic di-GMP phosphodiesterase PdeB {ECO:0000305};
DE EC=3.1.4.52 {ECO:0000250|UniProtKB:P21514};
GN Name=pdeB {ECO:0000303|PubMed:26148715}; Synonyms=ylaB;
GN OrderedLocusNames=b0457, JW5062;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP INDUCTION, AND RPOS-DEPENDENCE.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=19332833; DOI=10.1099/mic.0.024257-0;
RA Sommerfeldt N., Possling A., Becker G., Pesavento C., Tschowri N.,
RA Hengge R.;
RT "Gene expression patterns and differential input into curli fimbriae
RT regulation of all GGDEF/EAL domain proteins in Escherichia coli.";
RL Microbiology 155:1318-1331(2009).
RN [5]
RP NOMENCLATURE.
RX PubMed=26148715; DOI=10.1128/jb.00424-15;
RA Hengge R., Galperin M.Y., Ghigo J.M., Gomelsky M., Green J., Hughes K.T.,
RA Jenal U., Landini P.;
RT "Systematic nomenclature for GGDEF and EAL domain-containing cyclic di-GMP
RT turnover proteins of Escherichia coli.";
RL J. Bacteriol. 198:7-11(2015).
CC -!- FUNCTION: Phosphodiesterase (PDE) that catalyzes the hydrolysis of
CC cyclic-di-GMP (c-di-GMP) to 5'-pGpG. {ECO:0000250|UniProtKB:P21514}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyclic di-3',5'-guanylate + H2O = 5'-
CC phosphoguanylyl(3'->5')guanosine + H(+); Xref=Rhea:RHEA:24902,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58754,
CC ChEBI:CHEBI:58805; EC=3.1.4.52;
CC Evidence={ECO:0000250|UniProtKB:P21514};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- INDUCTION: Expressed during transition into stationary phase, at both
CC 28 and 37 degrees Celsius. Expression is RpoS dependent.
CC {ECO:0000269|PubMed:19332833}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB40212.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U82664; AAB40212.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC73559.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE76236.1; -; Genomic_DNA.
DR PIR; H64775; H64775.
DR RefSeq; NP_414990.2; NC_000913.3.
DR RefSeq; WP_001310610.1; NZ_SSUW01000008.1.
DR AlphaFoldDB; P77473; -.
DR SMR; P77473; -.
DR BioGRID; 4261748; 14.
DR STRING; 511145.b0457; -.
DR jPOST; P77473; -.
DR PaxDb; P77473; -.
DR PRIDE; P77473; -.
DR EnsemblBacteria; AAC73559; AAC73559; b0457.
DR EnsemblBacteria; BAE76236; BAE76236; BAE76236.
DR GeneID; 948951; -.
DR KEGG; ecj:JW5062; -.
DR KEGG; eco:b0457; -.
DR PATRIC; fig|1411691.4.peg.1819; -.
DR EchoBASE; EB3988; -.
DR eggNOG; COG4943; Bacteria.
DR HOGENOM; CLU_000445_131_1_6; -.
DR InParanoid; P77473; -.
DR OMA; HIIRMAH; -.
DR PhylomeDB; P77473; -.
DR BioCyc; EcoCyc:G6252-MON; -.
DR PRO; PR:P77473; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0071111; F:cyclic-guanylate-specific phosphodiesterase activity; IDA:EcoCyc.
DR CDD; cd01948; EAL; 1.
DR Gene3D; 3.20.20.450; -; 1.
DR InterPro; IPR024744; CSS-motif_dom.
DR InterPro; IPR001633; EAL_dom.
DR InterPro; IPR035919; EAL_sf.
DR Pfam; PF12792; CSS-motif; 1.
DR Pfam; PF00563; EAL; 1.
DR SMART; SM00052; EAL; 1.
DR SUPFAM; SSF141868; SSF141868; 1.
DR PROSITE; PS50883; EAL; 1.
PE 2: Evidence at transcript level;
KW c-di-GMP; Cell inner membrane; Cell membrane; Hydrolase; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..516
FT /note="Probable cyclic di-GMP phosphodiesterase PdeB"
FT /id="PRO_0000168633"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 268..516
FT /note="EAL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00074"
SQ SEQUENCE 516 AA; 58720 MW; 3FC1355E51CF6464 CRC64;
MRTRHLVGLI SGVLILSVLL PVGLSIWLAH QQVETSFIEE LDTYSSRVAI RANKVATQGK
DALQELERWQ GAACSEAHLM EMRRVSYSYR YIQEVAYIDN NVPQCSSLEH ESPPDTFPEP
GKISKDGYRV WLTSHNDLGI IRYMVAMGTA HYVVMIDPAS FIDVIPYSSW QIDAAIIGNA
HNVVITSSDE IAQGIITRLQ KTPGEHIENN GIIYDILPLP EMNISIITWA STKMLQKGWH
RQVFIWLPLG LVIGLLAAMF VLRILRRIQS PHHRLQDAIE NRDICVHYQP IVSLANGKIV
GAEALARWPQ TDGSWLSPDS FIPLAQQTGL SEPLTLLIIR SVFEDMGDWL RQHPQQHISI
NLESPVLTSE KIPQLLRDMI NHYQVNPRQI ALELTEREFA DPKTSAPIIS RYREAGHEIY
LDDFGTGYSS LSYLQDLDVD ILKIDKSFVD ALEYKNVTPH IIEMAKTLKL KMVAEGIETS
KQEEWLRQHG VHYGQGWLYS KALPKEDFLR WAEQHL
