PDF1_CAEEL
ID PDF1_CAEEL Reviewed; 88 AA.
AC G8JYC6; B2BBX9; G5EEV3; Q8WQD1;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2017, sequence version 1.
DT 03-AUG-2022, entry version 23.
DE RecName: Full=Pigment dispersing factor homolog pdf-1 {ECO:0000305};
DE Flags: Precursor;
GN Name=pdf-1 {ECO:0000312|WormBase:T07E3.6a};
GN ORFNames=T07E3.6 {ECO:0000312|WormBase:T07E3.6a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:ABO42258.1, ECO:0000312|EMBL:ABO42259.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=19686386; DOI=10.1111/j.1471-4159.2009.06323.x;
RA Janssen T., Husson S.J., Meelkop E., Temmerman L., Lindemans M.,
RA Verstraelen K., Rademakers S., Mertens I., Nitabach M., Jansen G.,
RA Schoofs L.;
RT "Discovery and characterization of a conserved pigment dispersing factor-
RT like neuropeptide pathway in Caenorhabditis elegans.";
RL J. Neurochem. 111:228-241(2009).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=18390545; DOI=10.1074/jbc.m709060200;
RA Janssen T., Husson S.J., Lindemans M., Mertens I., Rademakers S.,
RA Donck K.V., Geysen J., Jansen G., Schoofs L.;
RT "Functional characterization of three G protein-coupled receptors for
RT pigment dispersing factors in Caenorhabditis elegans.";
RL J. Biol. Chem. 283:15241-15249(2008).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=22579613; DOI=10.1016/j.mce.2012.05.001;
RA Meelkop E., Temmerman L., Janssen T., Suetens N., Beets I., Van Rompay L.,
RA Shanmugam N., Husson S.J., Schoofs L.;
RT "PDF receptor signaling in Caenorhabditis elegans modulates locomotion and
RT egg-laying.";
RL Mol. Cell. Endocrinol. 361:232-240(2012).
RN [5] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=23143519; DOI=10.1038/nn.3253;
RA Barrios A., Ghosh R., Fang C., Emmons S.W., Barr M.M.;
RT "PDF-1 neuropeptide signaling modulates a neural circuit for mate-searching
RT behavior in C. elegans.";
RL Nat. Neurosci. 15:1675-1682(2012).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=23972393; DOI=10.1016/j.cell.2013.08.001;
RA Flavell S.W., Pokala N., Macosko E.Z., Albrecht D.R., Larsch J.,
RA Bargmann C.I.;
RT "Serotonin and the neuropeptide PDF initiate and extend opposing behavioral
RT states in C. elegans.";
RL Cell 154:1023-1035(2013).
RN [7] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=23764289; DOI=10.1016/j.neuron.2013.04.002;
RA Choi S., Chatzigeorgiou M., Taylor K.P., Schafer W.R., Kaplan J.M.;
RT "Analysis of NPR-1 reveals a circuit mechanism for behavioral quiescence in
RT C. elegans.";
RL Neuron 78:869-880(2013).
RN [8] {ECO:0000305}
RP FUNCTION.
RX PubMed=26113231; DOI=10.1111/gbb.12231;
RA Herrero A., Romanowski A., Meelkop E., Caldart C.S., Schoofs L.,
RA Golombek D.A.;
RT "Pigment-dispersing factor signaling in the circadian system of
RT Caenorhabditis elegans.";
RL Genes Brain Behav. 14:493-501(2015).
RN [9] {ECO:0000305}
RP FUNCTION.
RX PubMed=27585848; DOI=10.1534/genetics.116.192898;
RA Chen D., Taylor K.P., Hall Q., Kaplan J.M.;
RT "The Neuropeptides FLP-2 and PDF-1 Act in Concert To Arouse Caenorhabditis
RT elegans Locomotion.";
RL Genetics 204:1151-1159(2016).
RN [10] {ECO:0000305}
RP FUNCTION.
RX PubMed=30024377; DOI=10.7554/elife.36547;
RA Hilbert Z.A., Kim D.H.;
RT "PDF-1 neuropeptide signaling regulates sexually dimorphic gene expression
RT in shared sensory neurons of C. elegans.";
RL Elife 7:0-0(2018).
CC -!- FUNCTION: Probable ligand of isoforms a and b of the calcitonin
CC receptor-like protein, pdfr-1, a G-protein coupled receptor
CC (PubMed:18390545). May not signal through isoform c of pdfr-1
CC (PubMed:18390545). Involved in locomotion; more specifically mate
CC searching behavior of males, independent of nutritional status
CC (PubMed:18390545, PubMed:22579613, PubMed:23143519, PubMed:30024377).
CC Involved in regulating the male-specific expression of TGFbeta-like
CC daf-7 in the ASJ chemosensory neurons (PubMed:30024377). Plays a role
CC in circadian rhythms of locomotor activity (PubMed:26113231). Involved
CC in mediating arousal from the sleep-like state called lethargus, which
CC occurs during molting between larval and adult stages, in part by
CC regulating touch sensitivity, and working in concert with neuropeptide
CC flp-2 (PubMed:23764289, PubMed:27585848). In the presence of food,
CC plays a role in initiating and extending exploratory roaming behavior,
CC in opposition to 5-hydroxytryptamine (serotonin) signaling.
CC {ECO:0000269|PubMed:18390545, ECO:0000269|PubMed:22579613,
CC ECO:0000269|PubMed:23143519, ECO:0000269|PubMed:23764289,
CC ECO:0000269|PubMed:23972393, ECO:0000269|PubMed:26113231,
CC ECO:0000269|PubMed:27585848, ECO:0000269|PubMed:30024377}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:T07E3.6a};
CC IsoId=G8JYC6-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:T07E3.6b};
CC IsoId=G8JYC6-2; Sequence=VSP_061252;
CC -!- DEVELOPMENTAL STAGE: Expressed throughout postembryonic life
CC (PubMed:19686386). Expressed in hermaphrodites in the interneurons
CC ADAL/R and PVT, the chemosensory neuron pairs ASI, ASK, PHA and PHB,
CC the RMED, RMEV and RID motor neurons, the sensory neuron pair ADE, the
CC PQR mechanosensory neuron and in the PVPL/R interneurons
CC (PubMed:19686386, PubMed:23764289). Also expressed in rectal gland
CC cells rectD and rectVL/R, and the intestino-rectal valve cells virL/R
CC (PubMed:19686386). Expressed in both sexes in the head interneurons
CC SAAV, SAAD, SIAV, AVB, AIM, RMG, and in the tail in the interneurons
CC PVN, LUA, PVT, and the pair PVS and PVU (referred to as PVP left and
CC right in the hermaphrodite) (PubMed:23143519, PubMed:23764289,
CC PubMed:23972393). Expressed in male-specific ventral cord neurons CP7
CC and CP8, and PDC (PubMed:23143519). Expression from the AIM
CC interneurons in males is involved in mate searching behavior
CC (PubMed:23143519). {ECO:0000269|PubMed:19686386,
CC ECO:0000269|PubMed:23143519, ECO:0000269|PubMed:23764289}.
CC -!- DISRUPTION PHENOTYPE: Conditional knockdown in AVB, PVP, and SIAV
CC neurons reduces exploratory roaming behavior.
CC {ECO:0000269|PubMed:23972393}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABO42258.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=ABO42259.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; EF141319; ABO42258.1; ALT_INIT; mRNA.
DR EMBL; EF141320; ABO42259.1; ALT_INIT; mRNA.
DR EMBL; BX284603; CCD72026.2; -; Genomic_DNA.
DR EMBL; BX284603; CCD72027.2; -; Genomic_DNA.
DR RefSeq; NP_741205.1; NM_171177.3.
DR RefSeq; NP_741206.1; NM_171878.3.
DR AlphaFoldDB; G8JYC6; -.
DR STRING; 6239.T07E3.6a; -.
DR EnsemblMetazoa; T07E3.6a.1; T07E3.6a.1; WBGene00020317. [G8JYC6-1]
DR EnsemblMetazoa; T07E3.6a.2; T07E3.6a.2; WBGene00020317. [G8JYC6-1]
DR EnsemblMetazoa; T07E3.6b.1; T07E3.6b.1; WBGene00020317. [G8JYC6-2]
DR GeneID; 175963; -.
DR CTD; 175963; -.
DR WormBase; T07E3.6a; CE52388; WBGene00020317; pdf-1.
DR WormBase; T07E3.6b; CE52158; WBGene00020317; pdf-1.
DR eggNOG; ENOG502SEYH; Eukaryota.
DR HOGENOM; CLU_1497564_0_0_1; -.
DR OrthoDB; 1579394at2759; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00020317; Expressed in larva and 3 other tissues.
DR ExpressionAtlas; G8JYC6; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IMP:UniProtKB.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IMP:UniProtKB.
DR GO; GO:0040011; P:locomotion; IMP:UniProtKB.
DR GO; GO:0031987; P:locomotion involved in locomotory behavior; IMP:UniProtKB.
DR GO; GO:0045475; P:locomotor rhythm; IMP:UniProtKB.
DR GO; GO:0060179; P:male mating behavior; IMP:UniProtKB.
DR GO; GO:0007567; P:parturition; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0050714; P:positive regulation of protein secretion; IGI:UniProtKB.
DR GO; GO:0030431; P:sleep; IMP:UniProtKB.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..88
FT /note="Pigment dispersing factor homolog pdf-1"
FT /evidence="ECO:0000255"
FT /id="PRO_5012903889"
FT VAR_SEQ 30..31
FT /note="Missing (in isoform b)"
FT /id="VSP_061252"
SQ SEQUENCE 88 AA; 9621 MW; EBE9444797E182BC CRC64;
MNRFIISMIA LLAVFCAVST ASPLLYRAPQ YQMYDDVQFV KRSNAELING LIGMDLGKLS
AVGKRSNAEL INGLLSMNLN KLSGAGRR
