ID   ASPT_TETHA              Reviewed;         543 AA.
AC   Q8L3K8;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Aspartate/alanine antiporter;
GN   Name=aspT;
OS   Tetragenococcus halophilus (Pediococcus halophilus).
OG   Plasmid pD1.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Tetragenococcus.
OX   NCBI_TaxID=51669;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=D10;
RX   PubMed=12003930; DOI=10.1128/jb.184.11.2906-2913.2002;
RA   Abe K., Ohnishi F., Yagi K., Nakajima T., Higuchi T., Sano M., Machida M.,
RA   Sarker R.I., Maloney P.C.;
RT   "Plasmid-encoded asp operon confers a proton motive metabolic cycle
RT   catalyzed by an aspartate-alanine exchange reaction.";
RL   J. Bacteriol. 184:2906-2913(2002).
CC   -!- FUNCTION: Catalyzes the electrogenic exchange of aspartate with
CC       alanine. {ECO:0000269|PubMed:12003930}.
CC   -!- INTERACTION:
CC       Q8L3K8; Q8L3K8: aspT; NbExp=2; IntAct=EBI-6406530, EBI-6406530;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the AAE transporter (TC 2.A.81) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB072729; BAB92081.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8L3K8; -.
DR   TCDB; 2.A.81.1.1; the aspartate:alanine exchanger (aaex) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   InterPro; IPR022457; Asp_Ala_antiprt.
DR   InterPro; IPR006512; YidE_YbjL.
DR   Pfam; PF06826; Asp-Al_Ex; 2.
DR   TIGRFAMs; TIGR03802; Asp_Ala_antiprt; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell membrane; Membrane; Plasmid; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..543
FT                   /note="Aspartate/alanine antiporter"
FT                   /id="PRO_0000208748"
FT   TRANSMEM        4..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        33..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        362..381
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        385..407
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        428..450
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        455..477
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        520..542
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   543 AA;  57213 MW;  3A86075EEEA942DA CRC64;
     MNAIGNFLVG TPVFTIFICL ALGYLLGKLK IGSFTLGATV GVLIVALLIG QLGVFPRDTL
     LGDIFFDFFM FAIGYRVGPS FISSMKKFGA KIVYATLIFL VSAFIVAYAC FKMFHIGPGI
     AAGIIAGGLT QSAVIGSSLE TISKLPISDH LKTLYSNQIP IVYTLTYVFG TIGVLIFLRD
     IMPKLMHIDL KKQAVKTAKE LDMIPVPVIV ASTHFYTIND GSSLIGQTLG TVNTKFAKGL
     VAAGLNDSAD MASVINAGDV LAISGGIDEI GRAVQEFNLL EVTGKTKAYV SKQVVLKKNF
     SADVLKNAQD KGVLVATLAG DVMDPAQFST LKPAESVTLV GQKDAVSEVQ SQLGRLRAAE
     NIINYSWFAL GIALSAALGI VGTKVSGVPI ALGGGTASLI VGLVQSIYRD KHAHMDTIPD
     SLLEFFQSIG LNLFIATVGL SAAKTFISAI QSMGISVLLI GAVISILPHI ITFVICYYLM
     KMEPISIIGA QTGADTLSAA LNDVSERVGS DASPFFAAAV APAYAIGNIF LTLMGPIFIV
     LLS