ASPX_HUMAN
ID ASPX_HUMAN Reviewed; 265 AA.
AC P26436; Q53FF4;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Acrosomal protein SP-10;
DE AltName: Full=Acrosomal vesicle protein 1;
DE Flags: Precursor;
GN Name=ACRV1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=1693291; DOI=10.1095/biolreprod42.4.693;
RA Wright R.M., John E., Klotz K., Flickinger C.J., Herr J.C.;
RT "Cloning and sequencing of cDNAs coding for the human intra-acrosomal
RT antigen SP-10.";
RL Biol. Reprod. 42:693-701(1990).
RN [2]
RP ERRATUM OF PUBMED:1693291, AND SEQUENCE REVISION.
RA Wright R.M., John E., Klotz K., Flickinger C.J., Herr J.C.;
RL Biol. Reprod. 43:903-903(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT ARG-126.
RX PubMed=8373955; DOI=10.1095/biolreprod49.2.316;
RA Wright R.M., Suri A.K., Kornreich B., Flickinger C.J., Herr J.C.;
RT "Cloning and characterization of the gene coding for the human acrosomal
RT protein SP-10.";
RL Biol. Reprod. 49:316-325(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ARG-126.
RC TISSUE=Testis;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP ALTERNATIVE SPLICING.
RC TISSUE=Testis;
RX PubMed=7619499; DOI=10.1002/mrd.1080410115;
RA Freemerman A.J., Flickinger C.J., Herr J.C.;
RT "Characterization of alternatively spliced human SP-10 mRNAs.";
RL Mol. Reprod. Dev. 41:100-108(1995).
RN [7]
RP PROTEIN SEQUENCE OF 78-100; 106-122 AND 127-151.
RX PubMed=1637938; DOI=10.1095/biolreprod47.1.11;
RA Herr J.C., Klotz K., Shannon J., Wright R.M., Flickinger C.J.;
RT "Purification and microsequencing of the intra-acrosomal protein SP-10.
RT Evidence that SP-10 heterogeneity results from endoproteolytic processes.";
RL Biol. Reprod. 47:11-20(1992).
CC -!- INTERACTION:
CC P26436; Q8N5K1: CISD2; NbExp=3; IntAct=EBI-25884472, EBI-1045797;
CC P26436; Q9BZ23-2: PANK2; NbExp=3; IntAct=EBI-25884472, EBI-25929070;
CC P26436; P50897: PPT1; NbExp=3; IntAct=EBI-25884472, EBI-1237011;
CC P26436; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-25884472, EBI-5235340;
CC P26436; Q86WV8: TSC1; NbExp=3; IntAct=EBI-25884472, EBI-12806590;
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome.
CC Note=Nascent acrosomal vesicle of Golgi phase spermatids.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=11;
CC Comment=Additional isoforms seem to exist.;
CC Name=1;
CC IsoId=P26436-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P26436-2; Sequence=VSP_004127;
CC Name=3;
CC IsoId=P26436-3; Sequence=VSP_004120;
CC Name=4;
CC IsoId=P26436-4; Sequence=VSP_004121;
CC Name=5;
CC IsoId=P26436-5; Sequence=VSP_004121, VSP_004127;
CC Name=6;
CC IsoId=P26436-6; Sequence=VSP_004122;
CC Name=7;
CC IsoId=P26436-7; Sequence=VSP_004120, VSP_004125;
CC Name=8;
CC IsoId=P26436-8; Sequence=VSP_004121, VSP_004128;
CC Name=9;
CC IsoId=P26436-9; Sequence=VSP_004121, VSP_004126;
CC Name=10;
CC IsoId=P26436-10; Sequence=VSP_004123;
CC Name=11;
CC IsoId=P26436-11; Sequence=VSP_004124;
CC -!- TISSUE SPECIFICITY: Testis.
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DR EMBL; M82967; AAA36625.1; -; mRNA.
DR EMBL; M82968; AAA36626.1; -; mRNA.
DR EMBL; S65583; AAB28238.2; -; Genomic_DNA.
DR EMBL; S65606; AAB28238.2; JOINED; Genomic_DNA.
DR EMBL; S65576; AAB28238.2; JOINED; Genomic_DNA.
DR EMBL; S65578; AAB28238.2; JOINED; Genomic_DNA.
DR EMBL; AK223335; BAD97055.1; -; mRNA.
DR EMBL; BC014588; AAH14588.1; -; mRNA.
DR CCDS; CCDS44759.1; -. [P26436-2]
DR CCDS; CCDS44761.1; -. [P26436-4]
DR CCDS; CCDS8460.1; -. [P26436-1]
DR CCDS; CCDS8461.1; -. [P26436-3]
DR PIR; A37225; A37225.
DR PIR; B37225; B37225.
DR RefSeq; NP_001603.1; NM_001612.5. [P26436-1]
DR RefSeq; NP_064454.1; NM_020069.4. [P26436-2]
DR RefSeq; NP_064492.1; NM_020107.4. [P26436-3]
DR RefSeq; NP_064493.1; NM_020108.4. [P26436-4]
DR AlphaFoldDB; P26436; -.
DR BioGRID; 106572; 2.
DR IntAct; P26436; 5.
DR STRING; 9606.ENSP00000432816; -.
DR GlyGen; P26436; 1 site.
DR BioMuta; ACRV1; -.
DR DMDM; 1351991; -.
DR MassIVE; P26436; -.
DR PaxDb; P26436; -.
DR PeptideAtlas; P26436; -.
DR PRIDE; P26436; -.
DR ProteomicsDB; 54335; -. [P26436-1]
DR ProteomicsDB; 54336; -. [P26436-10]
DR ProteomicsDB; 54337; -. [P26436-11]
DR ProteomicsDB; 54338; -. [P26436-2]
DR ProteomicsDB; 54339; -. [P26436-3]
DR ProteomicsDB; 54340; -. [P26436-4]
DR ProteomicsDB; 54341; -. [P26436-5]
DR ProteomicsDB; 54342; -. [P26436-6]
DR ProteomicsDB; 54343; -. [P26436-7]
DR ProteomicsDB; 54344; -. [P26436-8]
DR ProteomicsDB; 54345; -. [P26436-9]
DR Antibodypedia; 32960; 219 antibodies from 26 providers.
DR DNASU; 56; -.
DR Ensembl; ENST00000315608.7; ENSP00000317684.3; ENSG00000134940.14. [P26436-2]
DR Ensembl; ENST00000527795.1; ENSP00000436819.1; ENSG00000134940.14. [P26436-4]
DR Ensembl; ENST00000530048.5; ENSP00000433720.1; ENSG00000134940.14. [P26436-3]
DR Ensembl; ENST00000533904.6; ENSP00000432816.1; ENSG00000134940.14. [P26436-1]
DR GeneID; 56; -.
DR KEGG; hsa:56; -.
DR MANE-Select; ENST00000533904.6; ENSP00000432816.1; NM_001612.6; NP_001603.1.
DR UCSC; uc001qcl.4; human. [P26436-1]
DR CTD; 56; -.
DR DisGeNET; 56; -.
DR GeneCards; ACRV1; -.
DR HGNC; HGNC:127; ACRV1.
DR HPA; ENSG00000134940; Tissue enriched (testis).
DR MIM; 102525; gene.
DR neXtProt; NX_P26436; -.
DR OpenTargets; ENSG00000134940; -.
DR PharmGKB; PA24454; -.
DR VEuPathDB; HostDB:ENSG00000134940; -.
DR eggNOG; ENOG502QRB1; Eukaryota.
DR GeneTree; ENSGT00940000162351; -.
DR HOGENOM; CLU_070242_0_0_1; -.
DR InParanoid; P26436; -.
DR OMA; ENSQQCM; -.
DR OrthoDB; 1435784at2759; -.
DR PhylomeDB; P26436; -.
DR TreeFam; TF337781; -.
DR PathwayCommons; P26436; -.
DR SignaLink; P26436; -.
DR BioGRID-ORCS; 56; 14 hits in 1056 CRISPR screens.
DR GeneWiki; ACRV1; -.
DR GenomeRNAi; 56; -.
DR Pharos; P26436; Tbio.
DR PRO; PR:P26436; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; P26436; protein.
DR Bgee; ENSG00000134940; Expressed in left testis and 102 other tissues.
DR Genevisible; P26436; HS.
DR GO; GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0007283; P:spermatogenesis; NAS:UniProtKB.
DR CDD; cd00117; LU; 1.
DR InterPro; IPR016054; LY6_UPA_recep-like.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; Direct protein sequencing;
KW Glycoprotein; Reference proteome; Repeat; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..265
FT /note="Acrosomal protein SP-10"
FT /id="PRO_0000020760"
FT REPEAT 66..70
FT /note="1-1"
FT REPEAT 71..75
FT /note="1-2"
FT REPEAT 85..88
FT /note="2-1"
FT REPEAT 91..95
FT /note="1-3"
FT REPEAT 110..114
FT /note="3-1"
FT REPEAT 115..119
FT /note="3-2"
FT REPEAT 120..123
FT /note="2-2"
FT REPEAT 125..129
FT /note="3-3"
FT REPEAT 135..139
FT /note="3-4"
FT REPEAT 140..144
FT /note="3-5"
FT REPEAT 145..148
FT /note="2-3"
FT REPEAT 150..154
FT /note="3-6"
FT REPEAT 155..159
FT /note="3-7"
FT REPEAT 160..164
FT /note="3-8"
FT REPEAT 165..168
FT /note="2-4"
FT REPEAT 170..174
FT /note="3-9"
FT REGION 62..181
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 66..95
FT /note="3 X 5 AA repeats of S-E-H-[GA]-S"
FT REGION 85..168
FT /note="4 X 4 AA repeats of S-G-E-H"
FT REGION 110..174
FT /note="9 X 5 AA repeats of [SV]-G-E-Q-[PSA]"
FT COMPBIAS 71..110
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..181
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 258
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 41..224
FT /note="Missing (in isoform 11)"
FT /evidence="ECO:0000305"
FT /id="VSP_004124"
FT VAR_SEQ 41..184
FT /note="Missing (in isoform 10)"
FT /evidence="ECO:0000305"
FT /id="VSP_004123"
FT VAR_SEQ 41..135
FT /note="Missing (in isoform 6)"
FT /evidence="ECO:0000305"
FT /id="VSP_004122"
FT VAR_SEQ 41..110
FT /note="Missing (in isoform 4, isoform 5, isoform 8 and
FT isoform 9)"
FT /evidence="ECO:0000305"
FT /id="VSP_004121"
FT VAR_SEQ 41..95
FT /note="Missing (in isoform 3 and isoform 7)"
FT /evidence="ECO:0000305"
FT /id="VSP_004120"
FT VAR_SEQ 111..155
FT /note="Missing (in isoform 7)"
FT /evidence="ECO:0000305"
FT /id="VSP_004125"
FT VAR_SEQ 121..165
FT /note="Missing (in isoform 9)"
FT /evidence="ECO:0000305"
FT /id="VSP_004126"
FT VAR_SEQ 166..184
FT /note="Missing (in isoform 2 and isoform 5)"
FT /evidence="ECO:0000305"
FT /id="VSP_004127"
FT VAR_SEQ 185..224
FT /note="Missing (in isoform 8)"
FT /evidence="ECO:0000305"
FT /id="VSP_004128"
FT VARIANT 126
FT /note="G -> R (in dbSNP:rs34788353)"
FT /evidence="ECO:0000269|PubMed:8373955, ECO:0000269|Ref.4"
FT /id="VAR_050680"
FT CONFLICT 91
FT /note="S -> E (in Ref. 7; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 265 AA; 28156 MW; 318216453F20D68A CRC64;
MNRFLLLMSL YLLGSARGTS SQPNELSGSI DHQTSVQQLP GEFFSLENPS DAEALYETSS
GLNTLSEHGS SEHGSSKHTV AEHTSGEHAE SEHASGEPAA TEHAEGEHTV GEQPSGEQPS
GEHLSGEQPL SELESGEQPS DEQPSGEHGS GEQPSGEQAS GEQPSGEHAS GEQASGAPIS
STSTGTILNC YTCAYMNDQG KCLRGEGTCI TQNSQQCMLK KIFEGGKLQF MVQGCENMCP
SMNLFSHGTR MQIICCRNQS FCNKI
