PDHR_ECOLI
ID PDHR_ECOLI Reviewed; 254 AA.
AC P0ACL9; P06957; Q53381; Q8KMZ5;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Pyruvate dehydrogenase complex repressor;
GN Name=pdhR; Synonyms=aceC, genA, yacB; OrderedLocusNames=b0113, JW0109;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=6343085; DOI=10.1111/j.1432-1033.1983.tb07441.x;
RA Stephens P.E., Darlison M.G., Lewis H.M., Guest J.R.;
RT "The pyruvate dehydrogenase complex of Escherichia coli K12. Nucleotide
RT sequence encoding the pyruvate dehydrogenase component.";
RL Eur. J. Biochem. 133:155-162(1983).
RN [2]
RP SEQUENCE REVISION.
RA Quail M.A.;
RL Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP CHARACTERIZATION, AND VARIANT ACEC816.
RC STRAIN=K12;
RX PubMed=8262214; DOI=10.1016/0014-5793(93)81605-y;
RA Haydon D.J., Quail M.A., Guest J.R.;
RT "A mutation causing constitutive synthesis of the pyruvate dehydrogenase
RT complex in Escherichia coli is located within the pdhR gene.";
RL FEBS Lett. 336:43-47(1993).
CC -!- FUNCTION: Transcriptional repressor for the pyruvate dehydrogenase
CC complex genes aceEF and lpd.
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DR EMBL; V01498; CAA24739.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73224.1; -; Genomic_DNA.
DR EMBL; AP009048; BAB96683.2; -; Genomic_DNA.
DR EMBL; S67363; AAB29356.1; -; Genomic_DNA.
DR PIR; A64734; BVECA.
DR RefSeq; NP_414655.1; NC_000913.3.
DR RefSeq; WP_000331776.1; NZ_STEB01000010.1.
DR PDB; 5KVR; X-ray; 1.36 A; A=1-75.
DR PDB; 5TPM; X-ray; 2.80 A; A/B/C/D=99-254.
DR PDBsum; 5KVR; -.
DR PDBsum; 5TPM; -.
DR AlphaFoldDB; P0ACL9; -.
DR SMR; P0ACL9; -.
DR BioGRID; 4260661; 9.
DR BioGRID; 849227; 1.
DR IntAct; P0ACL9; 8.
DR STRING; 511145.b0113; -.
DR jPOST; P0ACL9; -.
DR PaxDb; P0ACL9; -.
DR PRIDE; P0ACL9; -.
DR EnsemblBacteria; AAC73224; AAC73224; b0113.
DR EnsemblBacteria; BAB96683; BAB96683; BAB96683.
DR GeneID; 67416185; -.
DR GeneID; 944827; -.
DR KEGG; ecj:JW0109; -.
DR KEGG; eco:b0113; -.
DR PATRIC; fig|1411691.4.peg.2169; -.
DR EchoBASE; EB1080; -.
DR eggNOG; COG2186; Bacteria.
DR HOGENOM; CLU_017584_9_5_6; -.
DR InParanoid; P0ACL9; -.
DR OMA; EWPVGSR; -.
DR PhylomeDB; P0ACL9; -.
DR BioCyc; EcoCyc:EG11088-MON; -.
DR PRO; PR:P0ACL9; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR CollecTF; EXPREG_00000860; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:EcoCyc.
DR CDD; cd07377; WHTH_GntR; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.20.120.530; -; 1.
DR InterPro; IPR011711; GntR_C.
DR InterPro; IPR008920; TF_FadR/GntR_C.
DR InterPro; IPR000524; Tscrpt_reg_HTH_GntR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF07729; FCD; 1.
DR Pfam; PF00392; GntR; 1.
DR PRINTS; PR00035; HTHGNTR.
DR SMART; SM00895; FCD; 1.
DR SMART; SM00345; HTH_GNTR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF48008; SSF48008; 1.
DR PROSITE; PS50949; HTH_GNTR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..254
FT /note="Pyruvate dehydrogenase complex repressor"
FT /id="PRO_0000050662"
FT DOMAIN 9..77
FT /note="HTH gntR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT DNA_BIND 37..56
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT VARIANT 118
FT /note="R -> C (in ACEC816; constitutively activated)"
FT HELIX 11..24
FT /evidence="ECO:0007829|PDB:5KVR"
FT HELIX 37..44
FT /evidence="ECO:0007829|PDB:5KVR"
FT HELIX 48..60
FT /evidence="ECO:0007829|PDB:5KVR"
FT STRAND 63..66
FT /evidence="ECO:0007829|PDB:5KVR"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:5KVR"
FT STRAND 72..74
FT /evidence="ECO:0007829|PDB:5KVR"
FT HELIX 99..118
FT /evidence="ECO:0007829|PDB:5TPM"
FT HELIX 121..139
FT /evidence="ECO:0007829|PDB:5TPM"
FT HELIX 143..160
FT /evidence="ECO:0007829|PDB:5TPM"
FT HELIX 164..188
FT /evidence="ECO:0007829|PDB:5TPM"
FT TURN 192..194
FT /evidence="ECO:0007829|PDB:5TPM"
FT HELIX 195..210
FT /evidence="ECO:0007829|PDB:5TPM"
FT HELIX 214..248
FT /evidence="ECO:0007829|PDB:5TPM"
SQ SEQUENCE 254 AA; 29425 MW; AF20245D3D3F751D CRC64;
MAYSKIRQPK LSDVIEQQLE FLILEGTLRP GEKLPPEREL AKQFDVSRPS LREAIQRLEA
KGLLLRRQGG GTFVQSSLWQ SFSDPLVELL SDHPESQYDL LETRHALEGI AAYYAALRST
DEDKERIREL HHAIELAQQS GDLDAESNAV LQYQIAVTEA AHNVVLLHLL RCMEPMLAQN
VRQNFELLYS RREMLPLVSS HRTRIFEAIM AGKPEEAREA SHRHLAFIEE ILLDRSREES
RRERSLRRLE QRKN
