ID   ASP_THECC               Reviewed;         221 AA.
AC   P32765;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=21 kDa seed protein;
DE   Flags: Precursor;
GN   Name=ASP;
OS   Theobroma cacao (Cacao) (Cocoa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Malvales; Malvaceae; Byttnerioideae; Theobroma.
OX   NCBI_TaxID=3641;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Seed;
RX   PubMed=1859871; DOI=10.1007/bf00015084;
RA   Tai H., McHenry L., Fritz P.J., Furtek D.B.;
RT   "Nucleic acid sequence of a 21 kDa cocoa seed protein with homology to the
RT   soybean trypsin inhibitor (Kunitz) family of protease inhibitors.";
RL   Plant Mol. Biol. 16:913-915(1991).
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
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DR   EMBL; X56509; CAA39860.1; -; mRNA.
DR   RefSeq; XP_007036743.1; XM_007036681.2.
DR   AlphaFoldDB; P32765; -.
DR   SMR; P32765; -.
DR   MEROPS; I03.030; -.
DR   EnsemblPlants; EOY21244; EOY21244; TCM_012658.
DR   GeneID; 18604292; -.
DR   Gramene; EOY21244; EOY21244; TCM_012658.
DR   KEGG; tcc:18604292; -.
DR   eggNOG; ENOG502QWSQ; Eukaryota.
DR   HOGENOM; CLU_090145_1_0_1; -.
DR   OMA; GNGWPFI; -.
DR   OrthoDB; 1343928at2759; -.
DR   GO; GO:0004866; F:endopeptidase inhibitor activity; IEA:InterPro.
DR   CDD; cd00178; STI; 1.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   PRINTS; PR00291; KUNITZINHBTR.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
DR   PROSITE; PS00283; SOYBEAN_KUNITZ; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..221
FT                   /note="21 kDa seed protein"
FT                   /id="PRO_0000016932"
FT   DISULFID        69..116
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   221 AA;  24039 MW;  2F1A8E76C5739EB1 CRC64;
     MKTATAVVLL LFAFTSKSYF FGVANAANSP VLDTDGDELQ TGVQYYVLSS ISGAGGGGLA
     LGRATGQSCP EIVVQRRSDL DNGTPVIFSN ADSKDDVVRV STDVNIEFVP IRDRLCSTST
     VWRLDNYDNS AGKWWVTTDG VKGEPGPNTL CSWFKIEKAG VLGYKFRFCP SVCDSCTTLC
     SDIGRHSDDD GQIRLALSDN EWAWMFKKAS KTIKQVVNAK H