PDLP3_ARATH
ID PDLP3_ARATH Reviewed; 304 AA.
AC O22784; Q94B68;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Plasmodesmata-located protein 3;
DE Short=PD-located protein 3 {ECO:0000303|PubMed:20886105};
DE AltName: Full=Cysteine-rich repeat secretory protein 11 {ECO:0000303|PubMed:11402176};
DE Flags: Precursor;
GN Name=PDLP3 {ECO:0000303|PubMed:20886105};
GN Synonyms=CRRSP11 {ECO:0000303|PubMed:11402176};
GN OrderedLocusNames=At2g33330; ORFNames=F4P9.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY ORGANIZATION, NOMENCLATURE, AND CAUTION.
RX PubMed=11402176; DOI=10.1104/pp.126.2.473;
RA Chen Z.;
RT "A superfamily of proteins with novel cysteine-rich repeats.";
RL Plant Physiol. 126:473-476(2001).
RN [5]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=19704520; DOI=10.4161/psb.3.10.6020;
RA Bayer E., Thomas C., Maule A.;
RT "Symplastic domains in the Arabidopsis shoot apical meristem correlate with
RT PDLP1 expression patterns.";
RL Plant Signal. Behav. 3:853-855(2008).
RN [6]
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=18215111; DOI=10.1371/journal.pbio.0060007;
RA Thomas C.L., Bayer E.M., Ritzenthaler C., Fernandez-Calvino L., Maule A.J.;
RT "Specific targeting of a plasmodesmal protein affecting cell-to-cell
RT communication.";
RL PLoS Biol. 6:E7-E7(2008).
RN [7]
RP SUBCELLULAR LOCATION, INTERACTION WITH GRAPEVINE FANLEAF VIRUS 2B-MP
RP PROTEIN, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=20886105; DOI=10.1371/journal.ppat.1001119;
RA Amari K., Boutant E., Hofmann C., Schmitt-Keichinger C.,
RA Fernandez-Calvino L., Didier P., Lerich A., Mutterer J., Thomas C.L.,
RA Heinlein M., Mely Y., Maule A.J., Ritzenthaler C.;
RT "A family of plasmodesmal proteins with receptor-like properties for plant
RT viral movement proteins.";
RL PLoS Pathog. 6:E1001119-E1001119(2010).
RN [8]
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=25393742; DOI=10.1371/journal.ppat.1004496;
RA Caillaud M.C., Wirthmueller L., Sklenar J., Findlay K., Piquerez S.J.,
RA Jones A.M., Robatzek S., Jones J.D., Faulkner C.;
RT "The plasmodesmal protein PDLP1 localises to haustoria-associated membranes
RT during downy mildew infection and regulates callose deposition.";
RL PLoS Pathog. 10:E1004496-E1004496(2014).
CC -!- FUNCTION: Modulates cell-to-cell trafficking.
CC {ECO:0000250|UniProtKB:Q8GXV7}.
CC -!- SUBUNIT: (Microbial infection) Interacts with Grapevine fanleaf virus
CC (GFLV) 2B-MP. {ECO:0000305|PubMed:20886105}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8GXV7};
CC Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q8GXV7}.
CC Cell junction, plasmodesma {ECO:0000269|PubMed:18215111,
CC ECO:0000269|PubMed:20886105, ECO:0000269|PubMed:25393742}. Note=Co-
CC localizes with the Grapevine fanleaf virus (GFLV) 2B-MP at the base of
CC tubules within modified plasmodesmata.
CC -!- TISSUE SPECIFICITY: Highly expressed in inflorescence pedacel and shoot
CC apex. Expressed in the outermost L1 layer of the shoot apical meristem
CC and in the epidermis of bulging floral primordia. Within the L1,
CC expression was restricted to the peripheral zone (at protein level).
CC {ECO:0000269|PubMed:19704520}.
CC -!- DEVELOPMENTAL STAGE: Expression in the epidermis of floral primordia
CC changes through development from being weakly expressed in the youngest
CC the floral organs, with possibly a stronger expression on the abaxial
CC side (P1, P2 stage) to being relatively strongly expressed in both the
CC adaxial and abaxial epidermis at later stages.
CC {ECO:0000269|PubMed:19704520}.
CC -!- DISRUPTION PHENOTYPE: Both pdlp2 and pdlp3, and pdlp1 and pdlp3 double
CC mutants show altered protein diffusion (measured using GFP). In pdlp1,
CC pdlp2 and pdlp3 triple mutant there is inhibition of GFLV 2BMP tubule
CC formation. Virus cell-to-cell movement is negatively affected. There is
CC a 22% reduction in mean surface area of infection foci by GFLV and an
CC approximately 12 h delay in long distance movement in comparison to
CC wild-type plants. There is also a systemic delay in Cauliflower mosaic
CC virus (CaMV) spread. {ECO:0000269|PubMed:18215111,
CC ECO:0000269|PubMed:20886105}.
CC -!- SIMILARITY: Belongs to the cysteine-rich repeat secretory protein
CC family. Plasmodesmata-located proteins (PDLD) subfamily. {ECO:0000305}.
CC -!- CAUTION: PDLPs were initially named Cysteine-rich secretory proteins
CC based on a classification work that failed to predict the transmembrane
CC region at the C-terminus (PubMed:11402176). However, it was later shown
CC that PDLPs are membrane proteins. {ECO:0000305}.
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DR EMBL; AC002332; AAB80651.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08816.1; -; Genomic_DNA.
DR EMBL; AY042813; AAK68753.1; -; mRNA.
DR EMBL; AY081690; AAM10252.1; -; mRNA.
DR PIR; B84744; B84744.
DR RefSeq; NP_565764.1; NM_128893.4.
DR AlphaFoldDB; O22784; -.
DR SMR; O22784; -.
DR BioGRID; 3243; 6.
DR IntAct; O22784; 5.
DR STRING; 3702.AT2G33330.1; -.
DR TCDB; 1.I.2.1.1; the plant plasmodesmata (ppd) family.
DR PaxDb; O22784; -.
DR PRIDE; O22784; -.
DR ProteomicsDB; 224523; -.
DR EnsemblPlants; AT2G33330.1; AT2G33330.1; AT2G33330.
DR GeneID; 817896; -.
DR Gramene; AT2G33330.1; AT2G33330.1; AT2G33330.
DR KEGG; ath:AT2G33330; -.
DR Araport; AT2G33330; -.
DR TAIR; locus:2051089; AT2G33330.
DR eggNOG; ENOG502QUWZ; Eukaryota.
DR HOGENOM; CLU_000288_33_1_1; -.
DR InParanoid; O22784; -.
DR OMA; NDCYNCV; -.
DR OrthoDB; 1077702at2759; -.
DR PhylomeDB; O22784; -.
DR PRO; PR:O22784; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22784; baseline and differential.
DR Genevisible; O22784; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; IDA:TAIR.
DR GO; GO:0010497; P:plasmodesmata-mediated intercellular transport; IGI:TAIR.
DR GO; GO:0046739; P:transport of virus in multicellular host; IGI:TAIR.
DR Gene3D; 3.30.430.20; -; 2.
DR InterPro; IPR002902; GNK2.
DR InterPro; IPR038408; GNK2_sf.
DR Pfam; PF01657; Stress-antifung; 2.
DR PROSITE; PS51473; GNK2; 2.
PE 1: Evidence at protein level;
KW Cell junction; Cell membrane; Disulfide bond; Host-virus interaction;
KW Membrane; Reference proteome; Repeat; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..304
FT /note="Plasmodesmata-located protein 3"
FT /id="PRO_0000296139"
FT TOPO_DOM 27..272
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..304
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT DOMAIN 34..138
FT /note="Gnk2-homologous 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DOMAIN 143..242
FT /note="Gnk2-homologous 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT REGION 273..293
FT /note="Necessary and sufficient for plasmodesmal targeting"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT DISULFID 41..116
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 92..101
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 104..129
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 151..220
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 196..205
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 208..233
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
SQ SEQUENCE 304 AA; 32658 MW; 795BB92F36018325 CRC64;
MGFYSLKQLL LLYIIIMALF SDLKLAKSSS PEYTNLIYKG CARQRLSDPS GLYSQALSAM
YGLLVTQSTK TRFYKTTTGT TSQTSVTGLF QCRGDLSNND CYNCVSRLPV LSGKLCGKTI
AARVQLSGCY LLYEISGFAQ ISGMELLFKT CGKNNVAGTG FEQRRDTAFG VMQNGVVQGH
GFYATTYESV YVLGQCEGDI GDSDCSGCIK NALQRAQVEC GSSISGQIYL HKCFVGYSFY
PNGVPKRSSP YPSSGSSGSS SSSSSSGTTG KTVAIIVGGT AGVGFLVICL LFVKNLMKKK
YDDY
