PDLP4_ARATH
ID PDLP4_ARATH Reviewed; 319 AA.
AC Q6E263; Q6E264; Q9M849; Q9M8Z3;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Plasmodesmata-located protein 4;
DE Short=PD-located protein 4 {ECO:0000303|PubMed:20886105};
DE AltName: Full=Cysteine-rich repeat secretory protein 39 {ECO:0000303|PubMed:11402176};
DE Flags: Precursor;
GN Name=PDLP4 {ECO:0000303|PubMed:20886105};
GN Synonyms=CRRSP39 {ECO:0000303|PubMed:11402176};
GN OrderedLocusNames=At3g04370; ORFNames=T27C4.1, T6K12.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RA Xiao Y.-L., Underwood B.A., Moskal W.A. Jr., Wang W., Redman J.C., Wu H.C.,
RA Utterback T., Town C.D.;
RT "Reconstruction of cDNA sequences for hypothetical genes in Arabidopsis
RT thaliana from 5' and 3' RACE products.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION, NOMENCLATURE, AND CAUTION.
RX PubMed=11402176; DOI=10.1104/pp.126.2.473;
RA Chen Z.;
RT "A superfamily of proteins with novel cysteine-rich repeats.";
RL Plant Physiol. 126:473-476(2001).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=19704520; DOI=10.4161/psb.3.10.6020;
RA Bayer E., Thomas C., Maule A.;
RT "Symplastic domains in the Arabidopsis shoot apical meristem correlate with
RT PDLP1 expression patterns.";
RL Plant Signal. Behav. 3:853-855(2008).
RN [7]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH GRAPEVINE FANLEAF VIRUS 2B-MP
RP PROTEIN.
RC STRAIN=cv. Columbia;
RX PubMed=20886105; DOI=10.1371/journal.ppat.1001119;
RA Amari K., Boutant E., Hofmann C., Schmitt-Keichinger C.,
RA Fernandez-Calvino L., Didier P., Lerich A., Mutterer J., Thomas C.L.,
RA Heinlein M., Mely Y., Maule A.J., Ritzenthaler C.;
RT "A family of plasmodesmal proteins with receptor-like properties for plant
RT viral movement proteins.";
RL PLoS Pathog. 6:E1001119-E1001119(2010).
CC -!- FUNCTION: Modulates cell-to-cell trafficking.
CC {ECO:0000250|UniProtKB:Q8GXV7}.
CC -!- SUBUNIT: (Microbial infection) Interacts with Grapevine fanleaf virus
CC (GFLV) 2B-MP. {ECO:0000305|PubMed:20886105}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8GXV7};
CC Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q8GXV7}.
CC Cell junction, plasmodesma {ECO:0000269|PubMed:20886105}. Note=Co-
CC localizes with the Grapevine fanleaf virus (GFLV) 2B-MP at the base of
CC tubules within modified plasmodesmata. {ECO:0000269|PubMed:20886105}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6E263-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6E263-2; Sequence=VSP_027132;
CC -!- TISSUE SPECIFICITY: Highly expressed in seeds and roots.
CC {ECO:0000269|PubMed:19704520}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cysteine-rich repeat secretory protein
CC family. Plasmodesmata-located proteins (PDLD) subfamily. {ECO:0000305}.
CC -!- CAUTION: PDLPs were initially named Cysteine-rich secretory proteins
CC based on a classification work that failed to predict the transmembrane
CC region at the C-terminus (PubMed:11402176). However, it was later shown
CC that PDLPs are membrane proteins. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF26777.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF63768.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAT68358.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAT68359.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAV63901.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC016829; AAF26777.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC022287; AAF63768.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74071.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74072.1; -; Genomic_DNA.
DR EMBL; AY600559; AAT68358.1; ALT_FRAME; mRNA.
DR EMBL; AY600560; AAT68359.1; ALT_FRAME; mRNA.
DR EMBL; AY773872; AAV63901.1; ALT_FRAME; mRNA.
DR RefSeq; NP_001030631.1; NM_001035554.2. [Q6E263-2]
DR RefSeq; NP_187087.2; NM_111308.3. [Q6E263-1]
DR AlphaFoldDB; Q6E263; -.
DR SMR; Q6E263; -.
DR STRING; 3702.AT3G04370.1; -.
DR TCDB; 1.I.2.1.1; the plant plasmodesmata (ppd) family.
DR PaxDb; Q6E263; -.
DR PRIDE; Q6E263; -.
DR ProteomicsDB; 220419; -. [Q6E263-1]
DR EnsemblPlants; AT3G04370.1; AT3G04370.1; AT3G04370. [Q6E263-1]
DR EnsemblPlants; AT3G04370.2; AT3G04370.2; AT3G04370. [Q6E263-2]
DR GeneID; 819592; -.
DR Gramene; AT3G04370.1; AT3G04370.1; AT3G04370. [Q6E263-1]
DR Gramene; AT3G04370.2; AT3G04370.2; AT3G04370. [Q6E263-2]
DR KEGG; ath:AT3G04370; -.
DR Araport; AT3G04370; -.
DR TAIR; locus:2100870; AT3G04370.
DR eggNOG; ENOG502R85K; Eukaryota.
DR InParanoid; Q6E263; -.
DR OMA; CTTNTFA; -.
DR OrthoDB; 1077702at2759; -.
DR PhylomeDB; Q6E263; -.
DR PRO; PR:Q6E263; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q6E263; baseline and differential.
DR Genevisible; Q6E263; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; IDA:TAIR.
DR GO; GO:0010497; P:plasmodesmata-mediated intercellular transport; IBA:GO_Central.
DR GO; GO:0046739; P:transport of virus in multicellular host; IBA:GO_Central.
DR Gene3D; 3.30.430.20; -; 2.
DR InterPro; IPR002902; GNK2.
DR InterPro; IPR038408; GNK2_sf.
DR Pfam; PF01657; Stress-antifung; 1.
DR PROSITE; PS51473; GNK2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell junction; Cell membrane; Disulfide bond;
KW Host-virus interaction; Membrane; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..319
FT /note="Plasmodesmata-located protein 4"
FT /id="PRO_0000296167"
FT TOPO_DOM 27..288
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 310..319
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT DOMAIN 45..149
FT /note="Gnk2-homologous 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DOMAIN 170..269
FT /note="Gnk2-homologous 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT REGION 289..309
FT /note="Necessary and sufficient for plasmodesmal targeting"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT DISULFID 39..127
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 103..112
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 115..140
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 177..247
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 223..232
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 235..260
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT VAR_SEQ 155..156
FT /note="QV -> L (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_027132"
SQ SEQUENCE 319 AA; 35264 MW; 65D12679A799F836 CRC64;
MVVHLISLLT QTLALIILSL PSIINTSQLD YDTLVFKQCD PLDANILQKA TTKSPNYSNQ
NLFLRAQALS SFLRKLESES SRSKFLKTLV GNEKHAVSGW FQCREDYPSE ICHKCVGDLR
EISSRSCGNA TSARIHLRGC HLIYKFERID TPGAQVNNHH KYKLFETPEH GLIHKICDGA
TAETFPGFEE MRTEALTAAE TGVVDGHGFY EDSYKLLHVV AQCDGHVEAC DCGECISSAA
AAAAEECRWS IAGQIYLEGC HVGYTYHPHE LPNDSYHEEG SKVNTGKSLA IVVGGVAALV
FVAIFFMFLK SLRKKGDDC
