PDLP6_ARATH
ID PDLP6_ARATH Reviewed; 288 AA.
AC Q9ZU94; Q0WTE1; Q3EC88; Q8LDG0; Q8W466;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Plasmodesmata-located protein 6;
DE Short=PD-located protein 6 {ECO:0000303|PubMed:20886105};
DE AltName: Full=Cysteine-rich repeat secretory protein 12 {ECO:0000303|PubMed:11402176};
DE Flags: Precursor;
GN Name=PDLP6 {ECO:0000303|PubMed:20886105};
GN Synonyms=CRRSP12 {ECO:0000303|PubMed:11402176};
GN OrderedLocusNames=At2g01660; ORFNames=T8O11.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 123-288 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY ORGANIZATION, NOMENCLATURE, AND CAUTION.
RX PubMed=11402176; DOI=10.1104/pp.126.2.473;
RA Chen Z.;
RT "A superfamily of proteins with novel cysteine-rich repeats.";
RL Plant Physiol. 126:473-476(2001).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=19704520; DOI=10.4161/psb.3.10.6020;
RA Bayer E., Thomas C., Maule A.;
RT "Symplastic domains in the Arabidopsis shoot apical meristem correlate with
RT PDLP1 expression patterns.";
RL Plant Signal. Behav. 3:853-855(2008).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=18215111; DOI=10.1371/journal.pbio.0060007;
RA Thomas C.L., Bayer E.M., Ritzenthaler C., Fernandez-Calvino L., Maule A.J.;
RT "Specific targeting of a plasmodesmal protein affecting cell-to-cell
RT communication.";
RL PLoS Biol. 6:E7-E7(2008).
RN [9]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH GRAPEVINE FANLEAF VIRUS 2B-MP
RP PROTEIN.
RC STRAIN=cv. Columbia;
RX PubMed=20886105; DOI=10.1371/journal.ppat.1001119;
RA Amari K., Boutant E., Hofmann C., Schmitt-Keichinger C.,
RA Fernandez-Calvino L., Didier P., Lerich A., Mutterer J., Thomas C.L.,
RA Heinlein M., Mely Y., Maule A.J., Ritzenthaler C.;
RT "A family of plasmodesmal proteins with receptor-like properties for plant
RT viral movement proteins.";
RL PLoS Pathog. 6:E1001119-E1001119(2010).
CC -!- FUNCTION: Modulates cell-to-cell trafficking.
CC {ECO:0000250|UniProtKB:Q8GXV7}.
CC -!- SUBUNIT: (Microbial infection) Interacts with Grapevine fanleaf virus
CC (GFLV) 2B-MP. {ECO:0000305|PubMed:20886105}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8GXV7};
CC Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q8GXV7}.
CC Cell junction, plasmodesma {ECO:0000269|PubMed:18215111,
CC ECO:0000269|PubMed:20886105}. Note=Co-localizes with the Grapevine
CC fanleaf virus (GFLV) 2B-MP at the base of tubules within modified
CC plasmodesmata.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9ZU94-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ZU94-2; Sequence=VSP_054963, VSP_054964;
CC -!- TISSUE SPECIFICITY: Highly expressed in inflorescence silique (at mRNA
CC level). {ECO:0000269|PubMed:19704520}.
CC -!- SIMILARITY: Belongs to the cysteine-rich repeat secretory protein
CC family. Plasmodesmata-located proteins (PDLD) subfamily. {ECO:0000305}.
CC -!- CAUTION: PDLPs were initially named Cysteine-rich secretory proteins
CC based on a classification work that failed to predict the transmembrane
CC region at the C-terminus (PubMed:11402176). However, it was later shown
CC that PDLPs are membrane proteins. {ECO:0000305}.
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DR EMBL; AC006069; AAD12705.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC05480.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05481.1; -; Genomic_DNA.
DR EMBL; AY062817; AAL32895.1; -; mRNA.
DR EMBL; BT006314; AAP13422.1; -; mRNA.
DR EMBL; AY086022; AAM63232.1; -; mRNA.
DR EMBL; AK227616; BAE99607.1; -; mRNA.
DR PIR; E84427; E84427.
DR RefSeq; NP_565272.1; NM_126227.3. [Q9ZU94-1]
DR RefSeq; NP_973394.1; NM_201665.2. [Q9ZU94-2]
DR AlphaFoldDB; Q9ZU94; -.
DR SMR; Q9ZU94; -.
DR BioGRID; 98; 7.
DR IntAct; Q9ZU94; 2.
DR STRING; 3702.AT2G01660.1; -.
DR TCDB; 1.I.2.1.1; the plant plasmodesmata (ppd) family.
DR PaxDb; Q9ZU94; -.
DR PRIDE; Q9ZU94; -.
DR ProteomicsDB; 224546; -. [Q9ZU94-1]
DR EnsemblPlants; AT2G01660.1; AT2G01660.1; AT2G01660. [Q9ZU94-1]
DR EnsemblPlants; AT2G01660.2; AT2G01660.2; AT2G01660. [Q9ZU94-2]
DR GeneID; 814695; -.
DR Gramene; AT2G01660.1; AT2G01660.1; AT2G01660. [Q9ZU94-1]
DR Gramene; AT2G01660.2; AT2G01660.2; AT2G01660. [Q9ZU94-2]
DR KEGG; ath:AT2G01660; -.
DR Araport; AT2G01660; -.
DR TAIR; locus:2065450; AT2G01660.
DR eggNOG; ENOG502QWR4; Eukaryota.
DR HOGENOM; CLU_000288_33_0_1; -.
DR InParanoid; Q9ZU94; -.
DR OMA; DLNNDQC; -.
DR OrthoDB; 1279011at2759; -.
DR PhylomeDB; Q9ZU94; -.
DR PRO; PR:Q9ZU94; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZU94; baseline and differential.
DR Genevisible; Q9ZU94; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; IDA:TAIR.
DR Gene3D; 3.30.430.20; -; 2.
DR InterPro; IPR002902; GNK2.
DR InterPro; IPR038408; GNK2_sf.
DR Pfam; PF01657; Stress-antifung; 2.
DR PROSITE; PS51473; GNK2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell junction; Cell membrane; Disulfide bond;
KW Host-virus interaction; Membrane; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..288
FT /note="Plasmodesmata-located protein 6"
FT /id="PRO_0000296140"
FT TOPO_DOM 23..256
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 278..288
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT DOMAIN 25..132
FT /note="Gnk2-homologous 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DOMAIN 137..234
FT /note="Gnk2-homologous 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT REGION 257..277
FT /note="Necessary and sufficient for plasmodesmal targeting"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT DISULFID 32..110
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 84..95
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 98..123
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 145..212
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 188..197
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 200..225
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT VAR_SEQ 243..259
FT /note="GNRNNNDDDEIEKTLAI -> NVFFAPLLFISFIKIFI (in isoform
FT 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_054963"
FT VAR_SEQ 260..288
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_054964"
FT CONFLICT 35
FT /note="E -> A (in Ref. 4; AAM63232)"
FT /evidence="ECO:0000305"
FT CONFLICT 39
FT /note="P -> Q (in Ref. 4; AAM63232)"
FT /evidence="ECO:0000305"
FT CONFLICT 68
FT /note="T -> A (in Ref. 4; AAM63232)"
FT /evidence="ECO:0000305"
FT CONFLICT 77
FT /note="S -> SS (in Ref. 4; AAM63232)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 288 AA; 30204 MW; CF894C9CE2604652 CRC64;
MFATKTVLFI AVVSLLGTFS SAAVDTFIYG GCSQEKYFPG SPYESNVNSL LTSFVSSASL
YTYNNFTTNG ISGDSSSVYG LYQCRGDLSS GSGDCARCVA RAVSRLGSLC AMASGGALQL
EGCFVKYDNT TFLGVEDKTV VVRRCGPPVG YNSDEMTRRD SVVGSLAASS GGSYRVGVSG
ELQGVAQCTG DLSATECQDC LMEAIGRLRT DCGGAAWGDV YLAKCYARYS ARGGHSRANG
YGGNRNNNDD DEIEKTLAII VGLIAGVTLL VVFLSFMAKS CERGKGGK
