PDLP7_ARATH
ID PDLP7_ARATH Reviewed; 298 AA.
AC Q0WPN8; Q501A0; Q9FHQ4;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Plasmodesmata-located protein 7;
DE Short=PD-located protein 7 {ECO:0000303|PubMed:20886105};
DE AltName: Full=Cysteine-rich repeat secretory protein 60 {ECO:0000303|PubMed:11402176};
DE Flags: Precursor;
GN Name=PDLP7 {ECO:0000303|PubMed:20886105};
GN Synonyms=CRRSP60 {ECO:0000303|PubMed:11402176};
GN OrderedLocusNames=At5g37660; ORFNames=K12B20.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis cDNA clones.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION, NOMENCLATURE, AND CAUTION.
RX PubMed=11402176; DOI=10.1104/pp.126.2.473;
RA Chen Z.;
RT "A superfamily of proteins with novel cysteine-rich repeats.";
RL Plant Physiol. 126:473-476(2001).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=19704520; DOI=10.4161/psb.3.10.6020;
RA Bayer E., Thomas C., Maule A.;
RT "Symplastic domains in the Arabidopsis shoot apical meristem correlate with
RT PDLP1 expression patterns.";
RL Plant Signal. Behav. 3:853-855(2008).
RN [7]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH GRAPEVINE FANLEAF VIRUS 2B-MP
RP PROTEIN.
RC STRAIN=cv. Columbia;
RX PubMed=20886105; DOI=10.1371/journal.ppat.1001119;
RA Amari K., Boutant E., Hofmann C., Schmitt-Keichinger C.,
RA Fernandez-Calvino L., Didier P., Lerich A., Mutterer J., Thomas C.L.,
RA Heinlein M., Mely Y., Maule A.J., Ritzenthaler C.;
RT "A family of plasmodesmal proteins with receptor-like properties for plant
RT viral movement proteins.";
RL PLoS Pathog. 6:E1001119-E1001119(2010).
CC -!- FUNCTION: Modulates cell-to-cell trafficking.
CC {ECO:0000250|UniProtKB:Q8GXV7}.
CC -!- SUBUNIT: (Microbial infection) Interacts with Grapevine fanleaf virus
CC (GFLV) 2B-MP. {ECO:0000305|PubMed:20886105}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8GXV7};
CC Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q8GXV7}.
CC Cell junction, plasmodesma {ECO:0000269|PubMed:20886105}. Note=Co-
CC localizes with the Grapevine fanleaf virus (GFLV) 2B-MP at the base of
CC tubules within modified plasmodesmata. {ECO:0000269|PubMed:20886105}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0WPN8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0WPN8-2; Sequence=VSP_027133, VSP_027134;
CC -!- TISSUE SPECIFICITY: Highly expressed in lateral root and elongation
CC zone. {ECO:0000269|PubMed:19704520}.
CC -!- SIMILARITY: Belongs to the cysteine-rich repeat secretory protein
CC family. Plasmodesmata-located proteins (PDLD) subfamily. {ECO:0000305}.
CC -!- CAUTION: PDLPs were initially named Cysteine-rich secretory proteins
CC based on a classification work that failed to predict the transmembrane
CC region at the C-terminus (PubMed:11402176). However, it was later shown
CC that PDLPs are membrane proteins. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08312.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB018107; BAB08312.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94215.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94216.1; -; Genomic_DNA.
DR EMBL; BT022067; AAY27054.1; -; mRNA.
DR EMBL; AK229025; BAF00911.1; -; mRNA.
DR RefSeq; NP_001078675.1; NM_001085206.1. [Q0WPN8-1]
DR RefSeq; NP_198582.2; NM_123125.4. [Q0WPN8-2]
DR AlphaFoldDB; Q0WPN8; -.
DR SMR; Q0WPN8; -.
DR BioGRID; 18995; 1.
DR IntAct; Q0WPN8; 1.
DR STRING; 3702.AT5G37660.2; -.
DR TCDB; 1.I.2.1.1; the plant plasmodesmata (ppd) family.
DR PaxDb; Q0WPN8; -.
DR PRIDE; Q0WPN8; -.
DR ProteomicsDB; 224406; -. [Q0WPN8-1]
DR EnsemblPlants; AT5G37660.1; AT5G37660.1; AT5G37660. [Q0WPN8-2]
DR EnsemblPlants; AT5G37660.2; AT5G37660.2; AT5G37660. [Q0WPN8-1]
DR GeneID; 833744; -.
DR Gramene; AT5G37660.1; AT5G37660.1; AT5G37660. [Q0WPN8-2]
DR Gramene; AT5G37660.2; AT5G37660.2; AT5G37660. [Q0WPN8-1]
DR KEGG; ath:AT5G37660; -.
DR Araport; AT5G37660; -.
DR TAIR; locus:2151709; AT5G37660.
DR eggNOG; ENOG502QTDS; Eukaryota.
DR InParanoid; Q0WPN8; -.
DR OMA; YARYTTK; -.
DR OrthoDB; 1279011at2759; -.
DR PhylomeDB; Q0WPN8; -.
DR PRO; PR:Q0WPN8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q0WPN8; baseline and differential.
DR Genevisible; Q0WPN8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; IDA:TAIR.
DR Gene3D; 3.30.430.20; -; 2.
DR InterPro; IPR002902; GNK2.
DR InterPro; IPR038408; GNK2_sf.
DR Pfam; PF01657; Stress-antifung; 2.
DR PROSITE; PS51473; GNK2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell junction; Cell membrane; Disulfide bond;
KW Host-virus interaction; Membrane; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..298
FT /note="Plasmodesmata-located protein 7"
FT /id="PRO_0000296173"
FT TOPO_DOM 31..258
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 280..298
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT DOMAIN 35..139
FT /note="Gnk2-homologous 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DOMAIN 140..240
FT /note="Gnk2-homologous 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT REGION 259..279
FT /note="Necessary and sufficient for plasmodesmal targeting"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT DISULFID 42..117
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 93..102
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 105..130
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 152..218
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 194..203
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 206..231
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT VAR_SEQ 288
FT /note="D -> K (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_027133"
FT VAR_SEQ 289..298
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_027134"
SQ SEQUENCE 298 AA; 31603 MW; 7C47946C0C82704A CRC64;
MPMAKLRNII KTLSIFFFLI AATAPSLSSA TSATDTFVFG GCSQQKFSPA SAYESNLNSL
LTSLVNSATY SSYNNFTIMG SSSSDTARGL FQCRGDLSMP DCATCVARAV SQVGPLCPFT
CGGALQLAGC YIKYDNISFL GQEDKTVVLK KCGSSEGYNT DGISRRDAVL TELVNGGGYF
RAGGSGDVQG MGQCVGDLTV SECQDCLGTA IGRLKNDCGT AVFGDMFLAK CYARYSTDGA
QHYAKSHNYK TNYGGEKTFA IIIGLLAAVV LLIIFLLFLR GVCSRGGDFS ILHSFTLI
