PDLP8_ARATH
ID PDLP8_ARATH Reviewed; 279 AA.
AC Q6NKQ9; Q9LZZ1;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Plasmodesmata-located protein 8 {ECO:0000303|PubMed:28786767};
DE Short=PD-located protein 8 {ECO:0000303|PubMed:20886105};
DE AltName: Full=Cysteine-rich repeat secretory protein 15 {ECO:0000303|PubMed:11402176};
DE Flags: Precursor;
GN Name=PDLP8 {ECO:0000303|PubMed:20886105};
GN Synonyms=CRRSP15 {ECO:0000303|PubMed:11402176};
GN OrderedLocusNames=At3g60720; ORFNames=T4C21_130;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION, NOMENCLATURE, AND CAUTION.
RX PubMed=11402176; DOI=10.1104/pp.126.2.473;
RA Chen Z.;
RT "A superfamily of proteins with novel cysteine-rich repeats.";
RL Plant Physiol. 126:473-476(2001).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=19704520; DOI=10.4161/psb.3.10.6020;
RA Bayer E., Thomas C., Maule A.;
RT "Symplastic domains in the Arabidopsis shoot apical meristem correlate with
RT PDLP1 expression patterns.";
RL Plant Signal. Behav. 3:853-855(2008).
RN [7]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH GRAPEVINE FANLEAF VIRUS 2B-MP
RP PROTEIN.
RC STRAIN=cv. Columbia;
RX PubMed=20886105; DOI=10.1371/journal.ppat.1001119;
RA Amari K., Boutant E., Hofmann C., Schmitt-Keichinger C.,
RA Fernandez-Calvino L., Didier P., Lerich A., Mutterer J., Thomas C.L.,
RA Heinlein M., Mely Y., Maule A.J., Ritzenthaler C.;
RT "A family of plasmodesmal proteins with receptor-like properties for plant
RT viral movement proteins.";
RL PLoS Pathog. 6:E1001119-E1001119(2010).
RN [8]
RP INTERACTION WITH ACBP6, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=28786767; DOI=10.1080/15592324.2017.1359365;
RA Ye Z.W., Chen Q.F., Chye M.L.;
RT "Arabidopsis thaliana Acyl-CoA-binding protein ACBP6 interacts with
RT plasmodesmata-located protein PDLP8.";
RL Plant Signal. Behav. 12:E1359365-E1359365(2017).
CC -!- FUNCTION: Modulates cell-to-cell trafficking.
CC {ECO:0000250|UniProtKB:Q8GXV7}.
CC -!- SUBUNIT: Interacts with ACBP6; interaction occurs at the plasma
CC membrane. {ECO:0000269|PubMed:28786767}.
CC -!- SUBUNIT: (Microbial infection) Interacts with Grapevine fanleaf virus
CC (GFLV) 2B-MP. {ECO:0000305|PubMed:20886105}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28786767};
CC Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q8GXV7}.
CC Cell junction, plasmodesma {ECO:0000269|PubMed:20886105,
CC ECO:0000269|PubMed:28786767}. Note=Co-localizes with the Grapevine
CC fanleaf virus (GFLV) 2B-MP at the base of tubules within modified
CC plasmodesmata (PubMed:20886105). {ECO:0000269|PubMed:20886105}.
CC -!- TISSUE SPECIFICITY: Highly expressed in pollen, lateral root and
CC elongation zone (PubMed:19704520). Higher expression in the
CC reproductive tissues (flowers and buds) than in vegetative organs
CC (leaves and stems). High expression in shoot and root phloem companion
CC cells (at protein level) (PubMed:28786767).
CC {ECO:0000269|PubMed:19704520, ECO:0000269|PubMed:28786767}.
CC -!- DISRUPTION PHENOTYPE: Affects accumulation of ACBP6 in the phloem.
CC {ECO:0000269|PubMed:28786767}.
CC -!- SIMILARITY: Belongs to the cysteine-rich repeat secretory protein
CC family. Plasmodesmata-located proteins (PDLD) subfamily. {ECO:0000305}.
CC -!- CAUTION: PDLPs were initially named Cysteine-rich secretory proteins
CC based on a classification work that failed to predict the transmembrane
CC region at the C-terminus (PubMed:11402176). However, it was later shown
CC that PDLPs are membrane proteins. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB82676.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL162295; CAB82676.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE80100.1; -; Genomic_DNA.
DR EMBL; BT012634; AAT06453.1; -; mRNA.
DR EMBL; AK229791; BAF01623.1; -; mRNA.
DR PIR; T47883; T47883.
DR RefSeq; NP_001326963.1; NM_001340056.1.
DR RefSeq; NP_191631.2; NM_115936.4.
DR PDB; 6GRF; X-ray; 1.95 A; A/B/C=22-253.
DR PDBsum; 6GRF; -.
DR AlphaFoldDB; Q6NKQ9; -.
DR SMR; Q6NKQ9; -.
DR BioGRID; 10557; 51.
DR IntAct; Q6NKQ9; 50.
DR STRING; 3702.AT3G60720.1; -.
DR TCDB; 1.I.2.1.1; the plant plasmodesmata (ppd) family.
DR PaxDb; Q6NKQ9; -.
DR ProteomicsDB; 222647; -.
DR EnsemblPlants; AT3G60720.1; AT3G60720.1; AT3G60720.
DR GeneID; 825243; -.
DR Gramene; AT3G60720.1; AT3G60720.1; AT3G60720.
DR KEGG; ath:AT3G60720; -.
DR Araport; AT3G60720; -.
DR TAIR; locus:2101811; AT3G60720.
DR eggNOG; ENOG502QU9X; Eukaryota.
DR HOGENOM; CLU_000288_33_0_1; -.
DR InParanoid; Q6NKQ9; -.
DR OMA; QINLVCP; -.
DR PhylomeDB; Q6NKQ9; -.
DR PRO; PR:Q6NKQ9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q6NKQ9; baseline and differential.
DR Genevisible; Q6NKQ9; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; IBA:GO_Central.
DR Gene3D; 3.30.430.20; -; 2.
DR InterPro; IPR002902; GNK2.
DR InterPro; IPR038408; GNK2_sf.
DR Pfam; PF01657; Stress-antifung; 2.
DR PROSITE; PS51473; GNK2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Cell junction; Cell membrane; Disulfide bond;
KW Host-virus interaction; Membrane; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..279
FT /note="Plasmodesmata-located protein 8"
FT /id="PRO_0000296143"
FT TOPO_DOM 21..253
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 275..279
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT DOMAIN 27..135
FT /note="Gnk2-homologous 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DOMAIN 137..237
FT /note="Gnk2-homologous 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT REGION 254..274
FT /note="Necessary and sufficient for plasmodesmal targeting"
FT /evidence="ECO:0000250|UniProtKB:Q8GXV7"
FT DISULFID 34..113
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 89..98
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 101..126
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 148..215
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 191..200
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 203..228
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT STRAND 29..34
FT /evidence="ECO:0007829|PDB:6GRF"
FT STRAND 41..43
FT /evidence="ECO:0007829|PDB:6GRF"
FT HELIX 44..60
FT /evidence="ECO:0007829|PDB:6GRF"
FT STRAND 64..71
FT /evidence="ECO:0007829|PDB:6GRF"
FT STRAND 83..89
FT /evidence="ECO:0007829|PDB:6GRF"
FT HELIX 95..112
FT /evidence="ECO:0007829|PDB:6GRF"
FT STRAND 116..133
FT /evidence="ECO:0007829|PDB:6GRF"
FT STRAND 143..148
FT /evidence="ECO:0007829|PDB:6GRF"
FT HELIX 157..170
FT /evidence="ECO:0007829|PDB:6GRF"
FT STRAND 175..182
FT /evidence="ECO:0007829|PDB:6GRF"
FT STRAND 185..191
FT /evidence="ECO:0007829|PDB:6GRF"
FT HELIX 197..214
FT /evidence="ECO:0007829|PDB:6GRF"
FT STRAND 218..235
FT /evidence="ECO:0007829|PDB:6GRF"
SQ SEQUENCE 279 AA; 30528 MW; 38F76A4086151C81 CRC64;
MRRLFLFSLL FLFFYSSSSS RSSSESHIFI YGGCSPEKYT PNTPFESNRD TFLSSVVTSS
SDASFNSFAV GNDSSSSSSS SAVFGLYQCR DDLRSSDCSK CIQTSVDQIT LICPYSYGAS
LQLEGCFLRY ETNDFLGKPD TSLRYKKCSS KSVENDYDFF KRRDDVLSDL ESTQLGYKVS
RSGLVEGYAQ CVGDLSPSDC TACLAESVGK LKNLCGSAVA AEVYLAQCYA RYWGSGYYDF
SSDPTNGDHV GKSIAIIVGV IAGFAILVVL LSLCRNSMH
