PDM2B_DROME
ID PDM2B_DROME Reviewed; 893 AA.
AC Q9VK71; Q6NR25;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=POU domain protein 2, isoform B;
DE AltName: Full=Miti-mere;
DE AltName: Full=Pdm-2;
DE AltName: Full=Protein didymous;
DE AltName: Full=dOct2;
DE AltName: Full=dPOU-28;
GN Name=pdm2 {ECO:0000312|EMBL:AAF53208.2};
GN Synonyms=dim {ECO:0000303|PubMed:1496003},
GN OCT2 {ECO:0000303|PubMed:1685891}, pdm-2 {ECO:0000303|PubMed:1680380},
GN POU-28 {ECO:0000303|PubMed:1881906}; ORFNames=CG12287;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAF53208.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAF53208.2}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAQ23575.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAQ23575.1}; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R {ECO:0000269|PubMed:1680380};
RC TISSUE=Embryo {ECO:0000269|PubMed:1680380};
RX PubMed=1680380; DOI=10.1016/0925-4773(91)90045-8;
RA Billin A.N., Cockerill K.A., Poole S.J.;
RT "Isolation of a family of Drosophila POU domain genes expressed in early
RT development.";
RL Mech. Dev. 34:75-84(1991).
RN [5] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=1685891; DOI=10.1016/0925-4773(91)90075-h;
RA Lloyd A., Sakonju S.;
RT "Characterization of two Drosophila POU domain genes, related to oct-1 and
RT oct-2, and the regulation of their expression patterns.";
RL Mech. Dev. 36:87-102(1991).
RN [6] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Embryo {ECO:0000269|PubMed:1881906};
RX PubMed=1881906; DOI=10.1073/pnas.88.17.7645;
RA Dick T., Yang X., Yeo S., Chia W.;
RT "Two closely linked Drosophila POU domain genes are expressed in
RT neuroblasts and sensory elements.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:7645-7649(1991).
RN [7] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Embryo {ECO:0000269|PubMed:1496003};
RX PubMed=1496003; DOI=10.1073/pnas.89.15.7080;
RA Prakash K., Fang X.D., Engelberg D., Behal A., Parker C.S.;
RT "dOct2, a Drosophila Oct transcription factor that functions in yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:7080-7084(1992).
CC -!- FUNCTION: DNA-binding regulatory protein implicated in early
CC development. Involved in neuronal cell fate decision. May act as an
CC octamer-dependent activator of transcription. Could also play an early
CC role in specific ectodermal cells, and a subsequent role in the
CC embryonic nervous system. {ECO:0000269|PubMed:1496003,
CC ECO:0000269|PubMed:1680380, ECO:0000269|PubMed:1685891,
CC ECO:0000269|PubMed:1881906}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B {ECO:0000269|PubMed:10731132};
CC IsoId=Q9VK71-1; Sequence=Displayed;
CC Name=A {ECO:0000269|PubMed:10731132};
CC IsoId=P31369-1; Sequence=External;
CC -!- TISSUE SPECIFICITY: Initial expression in cellular blastoderm stage,
CC then in ectodermal stripes during germband extension. Broad expression
CC in the neuroectoderm followed by limitation to discrete subsets of CNS
CC cells, and expression in specific PNS neurons and support cells.
CC {ECO:0000269|PubMed:1496003, ECO:0000269|PubMed:1680380,
CC ECO:0000269|PubMed:1685891, ECO:0000269|PubMed:1881906}.
CC -!- DEVELOPMENTAL STAGE: Expressed primarily during the first half of
CC embryogenesis. {ECO:0000269|PubMed:1680380,
CC ECO:0000269|PubMed:1685891}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-2
CC subfamily. {ECO:0000255}.
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DR EMBL; AE014134; AAF53208.2; -; Genomic_DNA.
DR EMBL; BT010257; AAQ23575.1; -; mRNA.
DR RefSeq; NP_001285877.1; NM_001298948.1. [Q9VK71-1]
DR RefSeq; NP_001285878.1; NM_001298949.1. [Q9VK71-1]
DR RefSeq; NP_723763.1; NM_165017.2. [Q9VK71-1]
DR AlphaFoldDB; Q9VK71; -.
DR SMR; Q9VK71; -.
DR BioGRID; 60717; 13.
DR IntAct; Q9VK71; 1.
DR STRING; 7227.FBpp0079973; -.
DR PaxDb; Q9VK71; -.
DR DNASU; 34673; -.
DR EnsemblMetazoa; FBtr0080392; FBpp0079973; FBgn0004394. [Q9VK71-1]
DR EnsemblMetazoa; FBtr0340539; FBpp0309438; FBgn0004394. [Q9VK71-1]
DR EnsemblMetazoa; FBtr0340540; FBpp0309439; FBgn0004394. [Q9VK71-1]
DR GeneID; 34673; -.
DR KEGG; dme:Dmel_CG12287; -.
DR CTD; 34673; -.
DR FlyBase; FBgn0004394; pdm2.
DR VEuPathDB; VectorBase:FBgn0004394; -.
DR eggNOG; KOG3802; Eukaryota.
DR HOGENOM; CLU_013065_5_0_1; -.
DR InParanoid; Q9VK71; -.
DR OMA; APNGRHC; -.
DR PhylomeDB; Q9VK71; -.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-DME-9018519; Estrogen-dependent gene expression.
DR BioGRID-ORCS; 34673; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 34673; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0004394; Expressed in presumptive embryonic/larval nervous system and 99 other tissues.
DR ExpressionAtlas; Q9VK71; baseline and differential.
DR Genevisible; Q9VK71; DM.
DR GO; GO:0005634; C:nucleus; IMP:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IMP:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007417; P:central nervous system development; IMP:UniProtKB.
DR GO; GO:0007398; P:ectoderm development; IMP:UniProtKB.
DR GO; GO:0048699; P:generation of neurons; IMP:FlyBase.
DR GO; GO:0014019; P:neuroblast development; IMP:FlyBase.
DR GO; GO:2000177; P:regulation of neural precursor cell proliferation; IGI:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Developmental protein; DNA-binding;
KW Homeobox; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..893
FT /note="POU domain protein 2, isoform B"
FT /id="PRO_0000308166"
FT DOMAIN 681..755
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 786..845
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 586..668
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 586..667
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 618
FT /note="R -> G (in Ref. 3; AAQ23575)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 893 AA; 98465 MW; CBA3E4A02B498EC1 CRC64;
MPLLLATTLA NNSHHQHLST TATETAAAAA TAKAAATAAA AATATMLLTS PTAATHCLAA
ATSAAGVNVQ ITGQGVSATL NDDQLQLPAN YAANVGHLNG FNPKDNCPPV PPTSERAFEG
LLRLPQIQVK QEFMESPPER PSCHPMNHQQ APANSALATS CRGKTILNPW YLRPADGIGY
HNYNSNNNNS GSGEINKDVS QQMTSACGGS DGQTCLPVSY FGMETLASVK QRQKSLPDFL
IPLHEIMFQQ QHHYQQRTMG TLINSLPPGL VGYPPQKTET FKSLTPPKAL QHHQNNQNHQ
IPQNHTGDHS FVRHFHERDR LIREHQLKHQ QLRDVEEVLQ EQEQDEEQEH ETLRKPHVVP
SELEHNHHWH GVVCDANGHA TRLLHIAPPR AGPTPLPTHQ QPPVGEVLPP NSTSFSTIVD
PPKEPSIYEQ PAPNGRHCRP ANKVMRHMSN SPTPPSPLRS LSDCGKSFEE EELELGENCE
MPQNLSSKRQ ARELDSELEN EVLDLAPPPK RLAEEQEEEK VASVNPPQPV AFAPEEMHQA
LQLQLHSYIE MVRQLAPEAF PNPNLATQFL LQNSLQALAQ FQALQQMKQQ QREDPLPSYS
TPLAKSPLRS PSLSPVPRHS KSQQRTPPNS MTANSLGMSS AVMTPNTPSM QQQPQLQQST
PKPTSGLTVA SAMAKLEQSP EETTDLEELE QFAKTFKQRR IKLGFTQGDV GLAMGKLYGN
DFSQTTISRF EALNLSFKNM CKLKPLLQKW LEDADSTVAK SGGGVFNINT MTSTLSSTPE
SILGRRRKKR TSIETTVRTT LEKAFLMNCK PTSEEISQLS ERLNMDKEVI RVWFCNRRQK
EKRINPSLDL DSPTGTPLSS HAFGYPPQAL NMSHMQMEGG SGSFCGSSIS SGE
