ID   ASR1_SARSH              Reviewed;         304 AA.
AC   A0A2U8U2M7;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   12-SEP-2018, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=MFS-type transporter asR1 {ECO:0000303|PubMed:29773797};
DE   AltName: Full=Xenovulene A biosynthesis cluster protein R1 {ECO:0000303|PubMed:29773797};
GN   Name=asR1 {ECO:0000303|PubMed:29773797};
OS   Sarocladium schorii (Acremonium strictum (strain IMI 501407)).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Sarocladiaceae; Sarocladium;
OC   unclassified Sarocladium.
OX   NCBI_TaxID=2203296;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, AND FUNCTION.
RX   PubMed=29773797; DOI=10.1038/s41467-018-04364-9;
RA   Schor R., Schotte C., Wibberg D., Kalinowski J., Cox R.J.;
RT   "Three previously unrecognised classes of biosynthetic enzymes revealed
RT   during the production of xenovulene A.";
RL   Nat. Commun. 9:1963-1963(2018).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=9262310;
RA   Thomas P., Sundaram H., Krishek B.J., Chazot P., Xie X., Bevan P.,
RA   Brocchini S.J., Latham C.J., Charlton P., Moore M., Lewis S.J.,
RA   Thornton D.M., Stephenson F.A., Smart T.G.;
RT   "Regulation of neuronal and recombinant GABA(A) receptor ion channels by
RT   xenovulene A, a natural product isolated from Acremonium strictum.";
RL   J. Pharmacol. Exp. Ther. 282:513-520(1997).
RN   [3]
RP   FUNCTION.
RX   PubMed=17912413; DOI=10.1039/b708614h;
RA   Bailey A.M., Cox R.J., Harley K., Lazarus C.M., Simpson T.J., Skellam E.;
RT   "Characterisation of 3-methylorcinaldehyde synthase (MOS) in Acremonium
RT   strictum: first observation of a reductive release mechanism during
RT   polyketide biosynthesis.";
RL   Chem. Commun. (Camb.) 39:4053-4055(2007).
RN   [4]
RP   FUNCTION.
RX   PubMed=20552126; DOI=10.1039/c0cc01162b;
RA   Fisch K.M., Skellam E., Ivison D., Cox R.J., Bailey A.M., Lazarus C.M.,
RA   Simpson T.J.;
RT   "Catalytic role of the C-terminal domains of a fungal non-reducing
RT   polyketide synthase.";
RL   Chem. Commun. (Camb.) 46:5331-5333(2010).
CC   -!- FUNCTION: MFS-type transporter; part of the gene cluster that mediates
CC       the biosynthesis of xenovulene A, an unusual meroterpenoid that has
CC       potent inhibitory effects on the human gamma-aminobutyrate A (GABAA)
CC       benzodiazepine receptor. {ECO:0000269|PubMed:29773797}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is significantly up-regulated under xenovulene A
CC       producing condition. {ECO:0000269|PubMed:29773797}.
CC   -!- BIOTECHNOLOGY: Xenovulene A is a natural product exhibiting little
CC       structural resemblance with classical benzodiazepines yet is able to
CC       displace high-affinity ligand binding to the benzodiazepine site of the
CC       gamma-aminobutyrate A (GABAA) receptor and could be potentially used as
CC       an anti-depressant with reduced addictive properties.
CC       {ECO:0000269|PubMed:9262310}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Monocarboxylate porter (TC 2.A.1.13) family. {ECO:0000305}.
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DR   EMBL; MG736817; AWM95790.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2U8U2M7; -.
DR   SMR; A0A2U8U2M7; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..304
FT                   /note="MFS-type transporter asR1"
FT                   /id="PRO_0000449187"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        132..152
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        166..186
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        248..268
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   304 AA;  33108 MW;  20577E50B2710569 CRC64;
     MEATAVSETR PLLESSEYPE DRRNHAETEL MPEEGLRGWL SVLGAWLCLF STFGLLSAVG
     FFQSTYQQTV LQAYGPDTIA WIFAIQLALL WAPGPFFGRL VDSYGPTPVL LPGSALCVLG
     LSLTSFSTEY YQIFLAQGVM FGIGAGGVFT AAQVCMAQWF VRRRGLAAGV AATGSSLGGA
     VFSIFLDRMK DLVGFRVAIR CAAALIGVCL MLALLVVRAR LPRAKWVAKL KWFDATIFKD
     RQFALYSTGA FLAMWGLWGP LAFIPGMVQN VGFPRSFSLE LLSILKYTTP HSPCISGSPC
     INID